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First Report of Meloidogyne incognita Infecting Mitragyna speciosa in the United States Cover

First Report of Meloidogyne incognita Infecting Mitragyna speciosa in the United States

Journal of Nematology
Volume 54 (2022): Issue 1 (February 2022)
By: Alemayehu Habteweld,  Wade Davidson,  Johan Desaeger and  William T. Crow  
Open Access
|Jul 2022

Figures & Tables

Figure 1

(A) Galled kratom (Mitragyna speciosa) root sample received from Hardee County, Florida; (B) a root-knot nematode female inside the kratom root sample.

Figure 2

(A) Two mitochondrial DNA regions span the intergenic spacer and part of the adjacent large subunit ribosomal RNA gene (lrDNA) amplified by TRNAH/MHR106 (Lanes 2 and 4, 557-bp) and MORF/MTHIS (Lanes 3 and 5, 742-bp) primer sets for sample 1 (S1) and sample 2 (S2); (B) amplified products using M. incognita-specific (MIF/MIR) (Lanes 2 and 4) and M. javanica-specific (Fjav/Rjav) (Lanes 3 and 5) primers. Only MIF/MIR primers yielded an amplified product approximately 999-bp which indicates the root-knot nematode that infected kratom was M. incognita. No product was observed from Fjav/Rjav primers. Lane 1 = 1 kb DNA ladder, with the position of the 500-bp band indicated by the arrow.
DOI: https://doi.org/10.2478/jofnem-2022-0021 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: Mar 8, 2022
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Published on: Jul 2, 2022
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Diagnosis,
Florida,
kratom,
mitochondrial haplotyping,
root galls,
root-knot nematode,
species-specific primers
Related subjects:
Life sciences,
Life sciences, other

© 2022 Alemayehu Habteweld, Wade Davidson, Johan Desaeger, William T. Crow, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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