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Belonolaimus longicaudatus Host Status and Pathogenicity on Sweetpotato Cover

Belonolaimus longicaudatus Host Status and Pathogenicity on Sweetpotato

Open Access
|Jul 2022

Figures & Tables

Figure 1

Soil sting nematode abundances at the termination of the greenhouse experiment as influenced by sting nematode (Belonolaimus longicaudatus Rau, 1958) inoculation rate and crop type including sweetpotato (Ipomoea batatas (L.) Lam.) cultivars, corn (Zea mays L.), and sunn hemp (Crotalaria juncea L.). In subfigure titles, “no inoculum,” “medium inoculum,” and “high inoculum” indicate non-inoculated, medium (26 and 40 sting nematodes per pot in Trials 1 and 2, respectively), and high rates of sting nematode inoculation (157 and 240 sting nematodes per pot in Trials 1 and 2, respectively). Letters indicate significant (Fisher’s protected LSD, P < 0.05) differences among crops within inoculation rate and trial. Values are means (N = 5) and standard errors.

Figure 2

Sting nematode (Belonolaimus longicaudatus Rau, 1958) reproductive factor (final divided by initial soil abundances) at the termination of the greenhouse experiment as influenced by crop type – including sweetpotato (Ipomoea batatas (L.) Lam.) cultivars, corn (Zea mays L.), and sunn hemp (Crotalaria juncea L.) – within sting nematode inoculation rate. The horizontal line in each pane indicates the reproductive factor is equal to 1, which generally delineates the host status. In subfigure titles, “medium” and “high” indicate medium (26 and 40 sting nematodes per pot in Trials 1 and 2, respectively) and high rates of sting nematode inoculation (157 and 240 sting nematodes per pot in Trials 1 and 2, respectively). Letters indicate significant (Fisher’s protected LSD, P < 0.05) differences among crops within inoculation rate and trial. Values are means (N = 5) and standard errors.

Table 1

Plant height (cm) of sweetpotato (Ipomoea batatas (L.) Lam.) cultivars, corn (Zea mays L.), and sunn hemp (Crotalaria juncea L.) under various sting nematode (Belonolaimus longicaudatus Rau, 1958) inoculation rates in greenhouse experiments.

CornaSunn hemp‘Beauregard’‘Covington’
Inoculation ratecPlant height 30 DAI: Trial 1b
Non-inoculated42.634.5 b41.026.2
Medium32.450.6 a26.039.0
High38.439.0 b35.236.5
Inoculation ratePlant height 30 DAI: Trial 2
Non-inoculated44.9 b36.327.519.0
Medium37.2 a32.927.823.9
High48.1 b27.126.425.2
Inoculation ratePlant height 70 DAI: Trial 1
Non-inoculated39.857.0 b67.138.5
Medium40.585.5 a47.839.4
High40.456.9 b76.143.0
Inoculation ratePlant height 68 DAI: Trial 2
Non-inoculated42.560.544.621.2
Medium45.455.752.931.3
High46.749.660.431.2

[i] aValues are means of five replicates. Letters within a crop, parameter, and run indicate significant differences (Fisher’s protected LSD, P < 0.05). Values with no letters are not significantly different (ANOVA, P < 0.05).

bDAI is days after inoculation.

cMedium and high inoculation rates were 26 and 157 sting nematodes per pot, respectively, in Trial 1, and 40 and 240 sting nematodes per pot, respectively, in Trial 2.

DAI, days after inoculation.

Table 2

Leaves per plant for sweetpotato (Ipomoea batatas (L.) Lam.) cultivars, corn (Zea mays L.), and sunn hemp (Crotalaria juncea L.) at various sting nematode (Belonolaimus longicaudatus Rau, 1958) inoculation rates in greenhouse experiments.

CornaSunn hemp‘Beauregard’‘Covington’
Inoculation ratecLeaves per plant 30 DAI: Trial 1b
Non-inoculated5137 b5
Medium7138 ab6
High5119 a6
Inoculation rateLeaves per plant 30 DAI: Trial 2
Non-inoculated6 b1396
Medium7 a1298
High6 b12107
Inoculation rateLeaves per plant 70 DAI: Trial 1
Non-inoculated11181010
Medium1023109
High1016139
Inoculation rateLeaves per plant 68 DAI: Trial 2
Non-inoculated921116
Medium919119
High1019149

[i] aValues are means of five replicates. Letters within a crop, parameter, and run indicate significant differences (Fisher’s protected LSD, P < 0.05). Values with no letters are not significantly different (ANOVA, P < 0.05).

bDAI is days after inoculation.

cMedium and high inoculation rates were 26 and 157 sting nematodes per pot, respectively, in Trial 1, and 40 and 240 sting nematodes per pot, respectively, in Trial 2.

DAI, days after inoculation.

Figure 3

Final shoot weight at the termination of the greenhouse experiment for different crops – including sweetpotato (Ipomoea batatas (L.) Lam.) cultivars, corn (Zea mays L.), and sunn hemp (Crotalaria juncea L.) – as influenced by sting nematode (Belonolaimus longicaudatus Rau, 1958) inoculation rate. “0,” “M,” and “H” indicate non-inoculated, medium (26 and 40 sting nematodes per pot in Trials 1 and 2, respectively), and high rates of sting nematode inoculation (157 and 240 sting nematodes per pot in Trials 1 and 2, respectively). Letters indicate significant (Fisher’s protected LSD, P < 0.05) differences among inoculation rates within crop and trial. Values are means (N = 5) and standard errors.

Figure 4

Final root weight at the termination of the greenhouse experiment for different crops – including sweetpotato (Ipomoea batatas (L.) Lam.) cultivars, corn (Zea mays, L.), and sunn hemp (Crotalaria juncea L.) – as influenced by sting nematode (Belonolaimus longicaudatus Rau, 1958) inoculation rate. “0,” “M,” and “H” indicate non-inoculated, medium (26 and 40 sting nematodes per pot in Trials 1 and 2, respectively), and high rates of sting nematode inoculation (157 and 240 sting nematodes per pot in Trials 1 and 2, respectively). Letters indicate significant (Fisher’s protected LSD, P < 0.05) differences among inoculation rates within crop and trial. Values are means (N = 5) and standard errors.

DOI: https://doi.org/10.2478/jofnem-2022-0019 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Feb 3, 2022
Published on: Jul 2, 2022
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2022 Zane J. Grabau, Chang Liu, Rebeca Sandoval-Ruiz, Wendy Mussoline, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.