Figure 1
Figure 2
Figure 3
Summary of experiments and the variables assessed_
| Variables assessed | |||||
|---|---|---|---|---|---|
| Life stagea | Takedown wkb | Pot size (g) | Inoculation densityc | Species | |
| Experiment 1 | Eggs, J2 | 8 | 100, 500, 3,000 | 0.5, 1, 2 | M. incognita |
| Experiment 2 | Eggs, J2 | 5, 6, 7, 8 | 500 | 1 | M. incognita |
| Experiment 3 | Eggs | 5, 6, 7, 8 | 500 | 1 | M. chitwoodi, M. hapla, M. incognita |
Summary of previous Meloidogyne species reproduction assay studies_
| Study | Species | Pot sizea | Inoculation densityb | Assay duration (degree day or time) | Experimental temperature | Reproduction factor or Gall ratingc |
|---|---|---|---|---|---|---|
| Pinkerton et al. (1991) | M. chitwoodi | 19 L | 150 eggs/250 cm3 | 1,750 DD | 21°C to 22°C | 20 |
| Pinkerton et al. (1991) | M. chitwoodi | 19 L | 150 eggs/250 cm3 | 2,000 DD | 21°C to 22°C | 120 |
| Ferris et al. (1993) | M. chitwoodi | 473 mL | 1,275 J2 | 10 wk | N/A | 16.07 |
| Ferris et al. (1993) | M. chitwoodi | 473 mL | 1,000 J2 | 90 d | N/A | 2.6–16.5 |
| Mojtahedi et al. (1988) | M. chitwoodi and M. hapla | 10 cm d | 5,000 eggs | 55 d | 22°C to 28°C | 47.3 |
| Santo and O’Bannon (1981) | M. chitwoodi and M. hapla | 18 cm d | 1,000 or 10,000 eggs | 13 wk | 15, 20, 25, and 30°C | M. chitwoodi: (6.9–442.8) M. hapla: (0.45–34) |
| Gaspard et al. (1990) | M. incognita | 172 cm3 | 800 J2 to 1,500 J2 | 1 mon | 500 DD20-30 | 0.4 |
| Gaspard et al. (1990) | M. incognita | 172 cm3 | 800 J2 to 1500 J2 | 3 mon | 500 DD20-30 | 0.4 |
| Ploeg and Maris (1999) | M. incognita | 1 L | 2,000 J2 | Varied | 16.2, 19.5, 25.0, 30.0, and 35.4°C. | 0.02–1.6 |
| Singh and Khurma (2008) | M. incognita | 2 L | 0, 200, 400, and 600 J2 | 8 wk | N/A | (avg # galls 13,19,26) (avg # egg masses 6, 11, and 17) |
| Silva et al. (2019) | M. incognita | 2500 cm3 | 5,000 eggs | 8 wk | 25°C | 17.2 |
| Cristóbal-Alejo et al. (2021) | M. incognita | 5 kg | 2,500 eggs and 1,625 J2 | 92 d | 30 ± 2°C | 1700 eggs/g of root, 45 females per gram of root, 150 galls per plant |
| Yeon et al. (2021) | M. incognita | 9.5 cm d | 10,000 eggs | 6 wk | 25 ± 5°C | 3.9 RF/9 gall index |
| Vega-Callo et al. (2021) | M. incognita and M. hapla | 3 kg | 5,000 eggs and J2 | 6 mon | 25 ± 5°C | M. incognita: (8.4) M. hapla (2.53) |
| Kankam and Adomako (2014) | Meloidogyne spp. | 1 L | 500, 1,000, and 2,000 J2 | 12 wk | 23.4–34.5°C | 1.2–1.7 |
| Wram and Zasada (2020) | M. incognita, M. chitwoodi, and M. hapla | 1,500 J2 | 15 wk | 26/18°C d/night | M. incognita: (61.2), M. chitwoodi: (25.5) M. hapla (32.2) | |
| El-Sherif et al. (2007) | M. incognita | 850 g | 250,500, and 1,000 eggs | 45 d | 23 ± 4°C | 1.1–1.5 |