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Morpho-Molecular and Ultrastructural Characterization of Discocriconemella parasinensis n. sp. from Zhejiang Province, China Cover

Morpho-Molecular and Ultrastructural Characterization of Discocriconemella parasinensis n. sp. from Zhejiang Province, China

Open Access
|May 2022

Figures & Tables

Figure 1

Line drawings of D. parasinensis n. sp. Female. (A) Esophageal region; (B, C) En face view of lip region; (D) Gonad; (E–G) Posterior region showing position of vulva and anus (Scale bars: A–C, E–G = 10 μm, D = 50 μm).
Line drawings of D. parasinensis n. sp. Female. (A) Esophageal region; (B, C) En face view of lip region; (D) Gonad; (E–G) Posterior region showing position of vulva and anus (Scale bars: A–C, E–G = 10 μm, D = 50 μm).

Figure 2

Light photomicrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B, C) Esophageal region arrowheads indicating position of base of esophageal bulb (ph.b) and excretory pore (exp); (D) Posterior region showing entire gonad arrowheads indicating position of vulva (v) and spermatheca (sp); (E) Mid-body (arrowhead pointing anastomoses); (F) Vulval region arrowhead showing spermatheca (sp); (G–I) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 100 μm, B–D = 50 μm, E–I = 10 μm).
Light photomicrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B, C) Esophageal region arrowheads indicating position of base of esophageal bulb (ph.b) and excretory pore (exp); (D) Posterior region showing entire gonad arrowheads indicating position of vulva (v) and spermatheca (sp); (E) Mid-body (arrowhead pointing anastomoses); (F) Vulval region arrowhead showing spermatheca (sp); (G–I) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 100 μm, B–D = 50 μm, E–I = 10 μm).

Figure 3

Scanning electron micrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B–F) Lip regions; (G) Cuticular annuli at mid-body; (H–J) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 50 μm; B–J = 10 μm).
Scanning electron micrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B–F) Lip regions; (G) Cuticular annuli at mid-body; (H–J) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 50 μm; B–J = 10 μm).

Figure 4

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18S rRNA gene sequence dataset with the TIM1+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18S rRNA gene sequence dataset with the TIM1+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 5

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2–D3 of 28S rRNA gene sequence dataset with the TIM3+I +G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2–D3 of 28S rRNA gene sequence dataset with the TIM3+I +G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 6

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Morphometric data of female of D_ parasinensis n_ sp_

CharacterHolotypeParatype
n15
L477.0486.1 ± 37.6 (382–556)
a7.87.7 ± 0.5 (6.7–9.0)
b4.04.3 ± 0.3 (3.6–4.6)
c15.516.4 ± 2.0 (13.8–20.6)
c′1.11.0 ± 0.1 (0.9–1.3)
V89.689.6 ± 0.8 (88.0–91.0)
VL/VB1.31.2 ± 0.1 (1.1–1.5)
R8282.6 ± 1.9 (80–86)
Rex2222.0 ± 0.7 (21.0–23.0)
RV1010.1 ± 0.4 (9–11)
RVan32.9 ± 0.2 (2–3)
Ran66.1 ± 0.3 (6–7)
Lip height9.07.8 ± 0.6 (7.0–9.0)
Lip diam.19.020.0 ± 1.0 (18.0–22.0)
Stylet length68.068.3 ± 4.9 (59.0–76.0)
Stylet (% L)14.314.1 ± 1.3 (12.3–16.8)
Pharynx119.0114.0 ± 5.8 (103.0–123.0)
Max. Body diam.61.063.2 ± 6.3 (51.0–72.0)
Vulval body diam.38.040.9 ± 3.6 (33.0–47.0)
Vulva to tail terminus49.050.4 ± 4.4 (41.0–56.0)
Anal body diam.28.030.0 ± 3.5 (24.0–35.0)
Tail length31.030.0 ± 3.9 (23.0–36.0)
DOI: https://doi.org/10.2478/jofnem-2022-0011 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Sep 9, 2021
Published on: May 18, 2022
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2022 Junxia Li, Maria Munawar, Pablo Castillo, Jingwu Zheng, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.