Skip to main content
Have a personal or library account? Click to login
Morpho-Molecular and Ultrastructural Characterization of Discocriconemella parasinensis n. sp. from Zhejiang Province, China Cover

Morpho-Molecular and Ultrastructural Characterization of Discocriconemella parasinensis n. sp. from Zhejiang Province, China

Open Access
|May 2022

Figures & Tables

Figure 1

Line drawings of D. parasinensis n. sp. Female. (A) Esophageal region; (B, C) En face view of lip region; (D) Gonad; (E–G) Posterior region showing position of vulva and anus (Scale bars: A–C, E–G = 10 μm, D = 50 μm).

Figure 2

Light photomicrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B, C) Esophageal region arrowheads indicating position of base of esophageal bulb (ph.b) and excretory pore (exp); (D) Posterior region showing entire gonad arrowheads indicating position of vulva (v) and spermatheca (sp); (E) Mid-body (arrowhead pointing anastomoses); (F) Vulval region arrowhead showing spermatheca (sp); (G–I) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 100 μm, B–D = 50 μm, E–I = 10 μm).

Figure 3

Scanning electron micrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B–F) Lip regions; (G) Cuticular annuli at mid-body; (H–J) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 50 μm; B–J = 10 μm).

Figure 4

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18S rRNA gene sequence dataset with the TIM1+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 5

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2–D3 of 28S rRNA gene sequence dataset with the TIM3+I +G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 6

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Morphometric data of female of D_ parasinensis n_ sp_

CharacterHolotypeParatype
n15
L477.0486.1 ± 37.6 (382–556)
a7.87.7 ± 0.5 (6.7–9.0)
b4.04.3 ± 0.3 (3.6–4.6)
c15.516.4 ± 2.0 (13.8–20.6)
c′1.11.0 ± 0.1 (0.9–1.3)
V89.689.6 ± 0.8 (88.0–91.0)
VL/VB1.31.2 ± 0.1 (1.1–1.5)
R8282.6 ± 1.9 (80–86)
Rex2222.0 ± 0.7 (21.0–23.0)
RV1010.1 ± 0.4 (9–11)
RVan32.9 ± 0.2 (2–3)
Ran66.1 ± 0.3 (6–7)
Lip height9.07.8 ± 0.6 (7.0–9.0)
Lip diam.19.020.0 ± 1.0 (18.0–22.0)
Stylet length68.068.3 ± 4.9 (59.0–76.0)
Stylet (% L)14.314.1 ± 1.3 (12.3–16.8)
Pharynx119.0114.0 ± 5.8 (103.0–123.0)
Max. Body diam.61.063.2 ± 6.3 (51.0–72.0)
Vulval body diam.38.040.9 ± 3.6 (33.0–47.0)
Vulva to tail terminus49.050.4 ± 4.4 (41.0–56.0)
Anal body diam.28.030.0 ± 3.5 (24.0–35.0)
Tail length31.030.0 ± 3.9 (23.0–36.0)
DOI: https://doi.org/10.2478/jofnem-2022-0011 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Sep 9, 2021
Published on: May 18, 2022
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2022 Junxia Li, Maria Munawar, Pablo Castillo, Jingwu Zheng, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.