Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax

Matczyszyn, Julianne N.; Harris, Timothy; Powers, Kirsten; Everhart, Sydney E.; Powers, Thomas O.

doi:10.2478/jofnem-2022-0009

Figures & Tables

A condensed maximum-likelihood tree of 721-bp sequences isolated from the COI gene for each individual in the morphospecies Mesocriconema xenoplax. Generated in the program MEGA, the tree displays seven partitions resulting in the HG label for further analysis. Bootstrap values from neighbor-joining and maximum-likelihood trees and posterior probability from Bayesian trees showing support for each HG are given at the terminal node, neighbor-joining, maximum-likelihood, and Bayesian, respectively. Each analysis is indicated by shading in the legend. The absence of the bar indicates the absence of support. The spanning tree network of each HG constructed using statistical parsimony is given within each HG bracket. Larger circles represent a unique haplotype. Smaller circles represent the number of mutational steps between haplotypes. Networks are disconnected when they exceed the 90% connection limit. HG, haplotype group.

Distribution of Mesocriconema xenoplax HG per collection site within the United States. The color of the circle is associated with the HG number, and the size of the circle is associated with the number of individuals of each HG collected at each site. HG, haplotype group.

An ordination plot using dbRDA bi-plot showing the seven most influential explanatory environmental variables (arrows) overlain on the first two eigenvectors of the analysis of Mesocriconema xenoplax HG. The length of the arrows is directly proportional to the strength of the correlation between the explanatory variable and the genetic variation calculated between individuals within each HG. Circles represent HG in ordination space, with the corresponding HG number indicated in the respective circle. Each axis indicates the amount of total variation each respective axis accounts for. dbRDA, distance-based redundancy analysis; HG, haplotype group.

Scatterplots of discriminant analysis of principal components, calculated on the category of land cover that distinguishes the sequential data the most. Points represent observed individual Mesocriconema xenoplax nematodes identified at their respective land cover type, connected to the population centroids. The center of each component is represented as black grid lines. Land cover types with fewer than five individual nematodes were removed from the analysis to reduce the amount of noise.

Stacked bar plot of the probability of each Mesocriconema xenoplax nematode to be reassigned to its respective population, defined according to land cover type; A. deciduous forest, B. developed open space, C. woody wetlands, D. cultivated crops, and E. herbaceous. Only those land cover types with ≥5 individuals were included in the analysis. The vertical axis is the probability (y-axis) of each individual (x-axis) being reassigned to its respective population, and the width of the bars depends on population size.

Canonical plot of the seven Mesocriconema xenoplax HG are indicated by color and significant morphological features: knob width (knob), total body length (L), and stylet length (Sty). Rays are scaled in the canonical plots to 1.5. The larger ellipses denote 50% of the observations, assuming normality, respective to size, for the mean canonical variables. The smaller ellipses represent a 95% confidence ellipsoid for the means on the canonical variable. HG, haplotype group.

Summary of morphological statistics by HG of Mesocriconema xenoplax specimens_

	HG 08	HG 09	HG 10	HG 11	HG 12	HG 13	HG 14
N	3	13	9	14	12	16	12
R	99 ± 2.6	94 ± 4.3	94 ± 3.9	101 ± 4.7	95 ± 3.4	98 ± 7.2	100 ± 3.9
Rv	7 ± 0.6	7 ± 0.9	6 ± 1.1	7 ± 0.9	7 ± 0.8	7 ± 1.2	7 ± 0.9
Rex	29 ± 2.1	27 ± 1.6	28 ± 1.9	27 ± 1.8	26 ± 1.6	27 ± 17	27 ± 1.9
Body annule width (µm)	6.4 ± 0.7	7.0 ± 1.0	7.3 ± 0.7	6.0 ± 0.7	6.8 ± 0.7	6.1 ± 0.6	7 ± 0.7
Length (µm)	598.2 ± 43.2	634.6 ± 83.2	621.6 ± 50.5	587.2 ± 63.0	604.8 ± 65.9	579.9 ± 49.3	642 ± 44.7
Stylet length (µm)	83.7 ± 2.1	87.6 ± 5.7	89.6 ± 3.8	72.6 ± 3.0	74.9 ± 3.6	73.3 ± 4.9	71 ± 1.4
Stylet knob width (µm)	13.3 ± 0.6	13.5 ± 0.9	13.3 ± 1.1	12.0 ± 0.7	12.3 ± 1.4	11.6 ± 1.3	14 ± 0.6
VUL	554.2 ± 41.3	594.2 ± 79.4	581.8 ± 52.3	549.7 ± 59.7	565.3 ± 61.0	539.4 ± 46.5	597 ± 40.1
MBW	52.7 ± 1.5	51.8 ± 4.8	55.8 ± 9.1	45.6 ± 6.7	45.6 ± 5.1	47.2 ± 5.6	52 ± 3.7
VBW	40.3 ± 2.5	38.0 ± 3.5	40.3 ± 5.9	35.0 ± 4.2	35.3 ± 4.5	36.7 ± 4.5	40 ± 4.6
ESO	145.7 ± 1.2	159.1 ± 13.7	159.6 ± 6.0	136.2 ± 7.0	141.0 ± 6.6	142.4 ± 6.1	146 ± 8.9
V	92.6 ± 0.8	93.6 ± 1.0	93.5 ± 1.2	93.6 ± 1.2	93.5 ± 0.6	93.0 ± 0.7	93 ± 1.2

Locality and GenBank accession information for Mesocriconema xenoplax specimens in this study_

NID	Locality	Ecoregion	Agricultural site (Y or N)	GenBank Accession No.
N3	Timmas Farm Ecological Forest Reserve, Cass County, NE, USA	Central Tall Grasslands	N	KJ787901
N173	Middle Loup River, Hooker County, NE, USA	Nebraska Sand Hills Mixed Grasslands	N	KJ787902
N583	Wakulla Springs State Park, Wakulla County, FL, USA	Southeastern Conifer Forests	N	KJ787880
N584	Ichetucknee Springs State Park, Columbia County, FL, USA	Southeastern Conifer Forests	N	KJ787885
N588	Ichetucknee Springs State Park, Columbia County, FL, USA	Southeastern Conifer Forests	N	KJ787886
N607	Schluckebier Prairie State Natural Area, Sauk County, WI, USA	Upper Midwest forest-savanna Transition Zone	N	KJ787881
N724	Chimney Creek, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KJ787906
N728	Pickens County, SC, USA	Southeastern Mixed Forests	Y	KJ787873
N729	Pickens County, SC, USA	Southeastern Mixed Forests	Y	KJ787874
N730	Pickens County, SC, USA	Southeastern Mixed Forests	Y	KJ787875
N733	Ozark National Forest, AR, USA	Central US Hardwood Forests	N	KJ787882
N735	Ozark National Forest, AR, USA	Central US Hardwood Forests	N	KJ787883
N736	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Mixed Forests	Y	KJ787887
N746	Crawford Bay, British Columbia, Canada	Cascade Mountains Leeward Forests	N	KJ787907
N747	Crawford Bay, British Columbia, Canada	Cascade Mountains Leeward Forests	N	KJ787908
N944	Timmas Farm Ecological Forest Reserve, Cass County, NE, USA	Central Tall Grasslands	N	KJ787903
N945	Timmas Farm Ecological Forest Reserve, Cass County, NE, USA	Central Tall Grasslands	N	KJ787904
N947	Timmas Farm Ecological Forest Reserve, Cass County, NE, USA	Central Tall Grasslands	N	KJ787905
N999	Albright Grove, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KJ787896
N1024	Albright Grove, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KJ787897
N1025	Albright Grove, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KJ787898
N1028	Albright Grove, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KJ787899
N1072	Nine-Mile Prairie, Lancaster County, NE, USA	Central Tall Grasslands	N	KJ787913
N1073	Nine-Mile Prairie, Lancaster County, NE, USA	Central Tall Grasslands	N	KJ787914
N1215	Long Branch Stream Valley Park, Fairfax County, VA, USA	Southeastern Mixed Forests	N	KJ787900
N1216	Long Branch Stream Valley Park, Fairfax County, VA, USA	Southeastern Mixed Forests	N	MN711175
N1217	Spring Green Prairie Preserve, Sauk County, WI, USA	Upper Midwest forest-savanna Transition Zone	N	KJ787884
N1267	George Washington Memorial Parkway, Fairfax County, VA, USA	Southeastern Mixed Forests	N	KJ787909
N1276	George Washington Memorial Parkway, Fairfax County, VA, USA	Southeastern Mixed Forests	N	KJ787910
N1294	Musser Fruit Research Farm, Oconee County, SC, USA	Southeastern Mixed Forests	Y	KJ787876
N1297	Musser Fruit Research Farm, Oconee County, SC, USA	Southeastern Mixed Forests	Y	KJ787877
N1298	Musser Fruit Research Farm, Oconee County, SC, USA	Southeastern Mixed Forests	Y	KJ787878
N1327	Fresno County, CA, USA	California Central Valley	Y	KU236636
N1346	George Washington Memorial Parkway, Fairfax County, VA, USA	Southeastern Mixed Forests	N	KY574831
N1361	George Washington Memorial Parkway, Fairfax County, VA, USA	Southeastern Mixed Forests	N	KY574643
N1368	Nine-Mile Prairie, Lancaster County, NE, USA	Central tall grasslands	N	KJ787915
N1375	Nine-Mile Prairie, Lancaster County, NE, USA	Central tall grasslands	N	KJ787916
N1397	George Washington Memorial Parkway, VA, USA	Southeastern Mixed Forests	N	KY574644
N1451	George Washington Memorial Parkway, VA, USA	Southeastern Mixed Forests	N	KJ788063
N2262	George Washington Memorial Parkway, VA, USA	Southeastern Mixed Forests	N	KY574645
N2269	George Washington Memorial Parkway, VA, USA	Southeastern Mixed Forests	N	MN711176
N2528	Nine-Mile Prairie, Lancaster County, NE, USA	Central tall grasslands	N	KY574650
N2557	Autauga County, AL, USA	Southeastern Mixed Forests	N	KY574633
N2558	Autauga County, AL, USA	Southeastern Mixed Forests	Y	MN711177
N2577	Chilton County, AL, USA	Southeastern Mixed Forests	Y	KY574624
N2604	Chilton County, AL, USA	Southeastern Mixed Forests	Y	MN711178
N2610	Chilton County, AL, USA	Southeastern Mixed Forests	Y	MN711179
N2611	Chilton County, AL, USA	Southeastern Mixed Forests	Y	MN711180
N2615	Chilton County, AL, USA	Southeastern Mixed Forests	Y	KY574634
N2618	Chilton County, AL, USA	Southeastern Mixed Forests	Y	KY574635
N2619	Chilton County, AL, USA	Southeastern Mixed Forests	Y	MN711181
N2622	Chilton County, AL, USA	Southeastern Mixed Forests	Y	KY574623
N2694	Albright Grove, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MF770909
N2719	Albright Grove, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711182
N2720	Albright Grove, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711183
N2727	Purchase Knob, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711184
N2728	Purchase Knob, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711185
N2842	Double Springs Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711186
N2844	Double Springs Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711187
N2849	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	KY574651
N2850	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	KY574652
N2851	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	MN711188
N2853	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	MN711189
N2855	Double Springs Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711190
N2857	Double Springs Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711191
N2858	Double Springs Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711192
N2863	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	KY574653
N2864	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	MN711193
N2869	Double Springs Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KY574646
N2873	Double Springs Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711194
N2874	Double Springs Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711195
N2891	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	KY574654
N2892	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	KY574655
N2893	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	MN711196
N2896	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	N	MN711197
N2933	West Point, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KY574647
N2945	West Point, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711198
N2998	Cades Cove, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711199
N3008	Goshen Prong, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KY574648
N3013	Trillium Gap, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711200
N3078	Gifford Woods State Park, VT, USA	New England Acadian Forests	N	KY574639
N3084	Hayden Prairie State Preserve, Howard County, IA, USA	Central tall grasslands	N	MN711201
N3112	Hayden Prairie State Preserve, Howard County, IA, USA	Central tall grasslands	N	MN711202
N3124	Hayden Prairie State Preserve, Howard County, IA, USA	Central tall grasslands	N	KY574656
N3153	George Washington Memorial Parkway, VA, USA	Southeastern Mixed Forests	N	KY574649
N3154	George Washington Memorial Parkway, VA, USA	Southeastern Mixed Forests	N	MN711203
N3208	Ichetucknee Springs State Park, FL, USA	Southeastern Conifer forests	N	KY574625
N3246	Oconaluftee, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711204
N3253	Oconaluftee, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711205
N3316	Twin Creeks, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KY574640
N3320	Twin Creeks, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	KY574832
N3342	Torreya State Park, FL, USA	Southeastern Conifer forests	N	KY574636
N3361	Raspberry Island, Apostle Islands National Lakeshore, WI, USA	Western Great Lakes Forest	N	KY574641
N3371	Raspberry Island, Apostle Islands National Lakeshore, WI, USA	Western Great Lakes Forest	N	MN711206
N3372	Raspberry Island, Apostle Islands National Lakeshore, WI, USA	Western Great Lakes Forest	N	MN711207
N3373	Raspberry Island, Apostle Islands National Lakeshore, WI, USA	Western Great Lakes Forest	N	MN711208
N3374	Raspberry Island, Apostle Islands National Lakeshore, WI, USA	Western Great Lakes Forest	N	MN711209
N3481	Wakulla Springs State Park, FL, USA	Southeastern Conifer forests	N	MN711210
N3491	West Point, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MF770951
N3492	West Point, Great Smoky Mountains National Park, USA	Appalachian Blue Ridge Forests	N	MN711211
N5508	Oconaluftee, Great Smoky Mountains National Park, USA	Appalachian-Blue Ridge Forests	N	MN711216
N5587	Tuskegee National Forest, Macon County, AL, USA	Southeastern Mixed Forests	N	KY574626
N5592	Canyonlands-South, Big Thicket National Preserve, TX, USA	Piney Woods forests	N	KY574627
N5593	Canyonlands-South, Big Thicket National Preserve, TX, USA	Piney Woods forests	N	KY574628
N5603	Canyonlands-South, Big Thicket National Preserve, TX, USA	Piney Woods forests	N	MF770959
N5638	Tuskegee National Forest, Macon County, AL, USA	Southeastern Mixed Forests	N	KY574629
N5643	Big Sandy Creek, Big Thicket National Preserve, TX, USA	Piney Woods forests	N	KY574630
N5645	Big Sandy Creek, Big Thicket National Preserve, TX, USA	Piney Woods forests	N	KY574631
N5712	Ichetucknee Springs State Park, FL, USA	Southeastern Conifer forests	N	MN711217
N5713	Ichetucknee Springs State Park, FL, USA	Southeastern Conifer forests	N	MN711218
N5714	Ichetucknee Springs State Park, FL, USA	Southeastern Conifer forests	N	MN711219
N5715	Ichetucknee Springs State Park, FL, USA	Southeastern Conifer forests	N	MN711220
N5726	Black Hills National Forest, Lawrence County, SD, USA	South Central Rockies forests	N	KY574642
N5727	Black Hills National Forest, Lawrence County, SD, USA	South Central Rockies forests	N	MN711221
N5728	Black Hills National Forest, Lawrence County, SD, USA	South Central Rockies forests	N	MN711222
N5731	Black Hills National Forest, Lawrence County, SD, USA	South Central Rockies forests	N	MN711223
N5813	Cuming County, NE, USA	Central Tall Grasslands	Y	KY574637
N5814	Cuming County, NE, USA	Central Tall Grasslands	Y	MN711224
N5815	Cuming County, NE, USA	Central Tall Grasslands	Y	MN711225
N5816	Cuming County, NE, USA	Central Tall Grasslands	Y	KY574632
N5943	Tulare County, CA, USA	Central California Valley	Y	KY574638
P74053	Fresno County, CA, USA	Central California Valley	Y	KJ787911
P194033	Konza Prairie Biological Station, Riley County, KS, USA	Flint Hills Tall Grasslands	Y	KJ787912
P231026	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787888
P231028	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787889
P231030	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787879
P231031	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787890
P231032	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787891
P231034	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787892
P231035	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787893
P231036	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787894
P231037	Southeastern Fruit and Nut Research Station, Peach County, GA, USA	Southeastern Plains	Y	KJ787895

Summary of genetic statistics for the 721-bp sequence isolated from the COI gene for each individual HG among the seven HG identified within the morphospecies Mesocriconema xenoplax_ HG are organized in the table below according to their phylogenetic relationships shown_

HG	n^a	S^b	PI^c	Hd^d	k^e	h^f	Pi^g
13	28	11	5	0.738	1.354	10	0.002
12	16	15	9	0.858	4.683	8	0.006
11	16	8	2	0.342	1.308	3	0.002
14	18	10	6	0.863	1.941	8	0.003
8	8	1	1	0.429	0.428	2	<0.001
10	23	7	3	0.711	1.628	6	0.002
9	20	60	50	0.932	19.53	13	0.027
Pooled	132	214	182	0.963	56.15	53	0.078

Population comparison defined by four categories of land use associated with the morphospecies Mesocriconema xenoplax in a pairwise AMOVA_

	Number of Individuals	Deciduous forest	Developed open space	Woody wetlands	Cultivated crops	Herbaceous
Deciduous forest	25	–	0.010	<0.001	0.011	<0.001
Developed open space	5	0.263	–	0.92	0.168	0.001
Woody wetlands	10	0.375	–0.114	–	0.027	<0.001
Cultivated crops	10	0.118	0.087	0.217	–	0.009
Herbaceous	9	0.654	0.725	0.757	0.330	–

Calculations of Birky’s 4× test on the morphospecies Mesocriconema xenoplax HG interclade divergence (K), intraclade variation (θ), and estimate of reciprocal monophyly (K/θ)_

HG	K^a	θ^b	K/θ^c
13	0.029	0.006	4.833^*
12	0.029	0.031	0.935
11	0.060	0.006	10.000^*
14	0.102	0.009	11.333^*
8	0.079	0.003	26.333^*
10	0.086	0.006	14.333^*
9	0.089	0.096	0.927

Species delimitation statistics corresponding to the neighbor-joining tree HG built from the COI gene sequence for the morphospecies Mesocriconema xenoplax_ Statistics include the intra-distance of each HG, inter-distance of each HG compared to its sister clade, ratio of intra- and inter-distance, P ID(strict), P ID(liberal), Av(MRCA-tips), Rodrigo’s P(AD), and Rosenberg’s P(AB)_ HG are organized in the table below according to their phylogenetic relationships shown_

HG	Closest HG	Intra-distance	Inter-distance - closest	Intra/inter	P ID (Strict)	P ID (Liberal)	Av(MRCA-tips)	P(AD)	R(AB)
13	12	0.004	0.03	0.13	0.95	0.98	0.002	0.24	7.00E−14
12	13	0.007	0.03	0.23	0.92	0.97	0.005	1.00	7.00E−14
11	13	0.002	0.063	0.02	0.99	1.00	0.004	0.05	3.60E−18
14	8	0.008	0.118	0.07	0.97	0.99	0.004	0.05	1.40E−24
8	13	<0.001	0.095	0.01	0.94	1.00	<0.001	0.05	6.70E−15
10	8	0.005	0.098	0.05	0.98	1.00	0.004	0.41	3.62E−03
9	8	0.03	0.104	0.29	0.90	0.97	0.020	0.56	3.90E−26

Isolation by distance metric calculated by Mantel test statistic (Mantel R), and associated P-value for each of the seven HG within the morphospecies Mesocriconema xenoplax_ HG are organized in the table below according to their phylogenetic relationships shown_

HG	Mantel R	P-value
13	0.048	0.731
12	0.206	0.118
11	0.989	<0.001^*
14	–0.108	0.443
8	0.874	0.021^*
10	0.627	<0.001^*
9	0.284	0.005^*

Ecological and morphological differentiation among COI haplotype groups in the plant parasitic nematode species Mesocriconema xenoplax

Figures & Tables

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5A–E

Figure 6

Summary of morphological statistics by HG of Mesocriconema xenoplax specimens_

Locality and GenBank accession information for Mesocriconema xenoplax specimens in this study_

Summary of genetic statistics for the 721-bp sequence isolated from the COI gene for each individual HG among the seven HG identified within the morphospecies Mesocriconema xenoplax_ HG are organized in the table below according to their phylogenetic relationships shown_

Population comparison defined by four categories of land use associated with the morphospecies Mesocriconema xenoplax in a pairwise AMOVA_

Calculations of Birky’s 4× test on the morphospecies Mesocriconema xenoplax HG interclade divergence (K), intraclade variation (θ), and estimate of reciprocal monophyly (K/θ)_

Isolation by distance metric calculated by Mantel test statistic (Mantel R), and associated P-value for each of the seven HG within the morphospecies Mesocriconema xenoplax_ HG are organized in the table below according to their phylogenetic relationships shown_

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