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Description of Boleodorus bushehrensis n. sp. (Rhabditida: Tylenchidae) from Southern Iran, and Observations on a Commonly Known Species Cover

Description of Boleodorus bushehrensis n. sp. (Rhabditida: Tylenchidae) from Southern Iran, and Observations on a Commonly Known Species

Open Access
|Apr 2022

Figures & Tables

Figure 1

Line drawings of Boleodorus bushehresnsis n. sp. (A, B, D, E, G: female; C, F: male). (A) Pharynx. (B) Posterior body region. (C) Anterior body region. (D) Vulval region, showing offset spermatheca. (E–G) Tail.
Line drawings of Boleodorus bushehresnsis n. sp. (A, B, D, E, G: female; C, F: male). (A) Pharynx. (B) Posterior body region. (C) Anterior body region. (D) Vulval region, showing offset spermatheca. (E–G) Tail.

Figure 2

Light microphotographs of Boleodorus bushehrensis n. sp. (A, B, C, E, F, I, J, K: female; D, G, H: male). (A, B) Anterior region showing cephalic region and stylet, respectively. (C) Pharyngeal metacorpus. (D) Anterior body region showing the oral aperture in a depression. (E, J) Pharyngeal bulb region showing excretory pore. (F, G, I) Tail tip. (H) Bursa. (K) Lateral field at midbody. (All scale bars = 10 μm).
Light microphotographs of Boleodorus bushehrensis n. sp. (A, B, C, E, F, I, J, K: female; D, G, H: male). (A, B) Anterior region showing cephalic region and stylet, respectively. (C) Pharyngeal metacorpus. (D) Anterior body region showing the oral aperture in a depression. (E, J) Pharyngeal bulb region showing excretory pore. (F, G, I) Tail tip. (H) Bursa. (K) Lateral field at midbody. (All scale bars = 10 μm).

Figure 3

Scanning electron microphotographs of Boleodorus bushehrensis n. sp. (female). (A) Anterior body region showing beginning of lateral field (arrow showing the excretory pore). (B–D) Anterior end in ventrolateral, ventral, and en face views, respectively (arrows pointing to the amphidial openings). (E, F) Excretory pore in lateral and ventral views, respectively (arrow). (G) Lateral field at vulva. (H, I) Vulva in lateral and ventral views, respectively. (J) Anterior body region in ventral view showing excretory pore. (K,N) Lateral field at midbody showing unusual division and four incisures, respectively. (L,M) Anus in ventral and lateral views, respectively.
Scanning electron microphotographs of Boleodorus bushehrensis n. sp. (female). (A) Anterior body region showing beginning of lateral field (arrow showing the excretory pore). (B–D) Anterior end in ventrolateral, ventral, and en face views, respectively (arrows pointing to the amphidial openings). (E, F) Excretory pore in lateral and ventral views, respectively (arrow). (G) Lateral field at vulva. (H, I) Vulva in lateral and ventral views, respectively. (J) Anterior body region in ventral view showing excretory pore. (K,N) Lateral field at midbody showing unusual division and four incisures, respectively. (L,M) Anus in ventral and lateral views, respectively.

Figure 4

Bayesian 50% majority rule consensus tree inferred from the SSU rDNA of Boleodorus bushehrensis n. sp. under the GTR + G + I model. Bayesian posterior probability values are given for corresponding clades. The new species is in bold font. GTR, general time-reversible; G, gamma; I, invariant; rDNA, ribosomal DNA; SSU, small subunit.
Bayesian 50% majority rule consensus tree inferred from the SSU rDNA of Boleodorus bushehrensis n. sp. under the GTR + G + I model. Bayesian posterior probability values are given for corresponding clades. The new species is in bold font. GTR, general time-reversible; G, gamma; I, invariant; rDNA, ribosomal DNA; SSU, small subunit.

Figure 5

Bayesian 50% majority rule consensus tree inferred from the LSU rDNA D2–D3 sequences of Boleodorus bushehrensis n. sp. under the GTR + G + I model. Bayesian posterior probability values are given for the corresponding clades. The new species is in bold font. GTR, general time-reversible; G, gamma; I, invariant; LSU, large subunit; rDNA, ribosomal DNA.
Bayesian 50% majority rule consensus tree inferred from the LSU rDNA D2–D3 sequences of Boleodorus bushehrensis n. sp. under the GTR + G + I model. Bayesian posterior probability values are given for the corresponding clades. The new species is in bold font. GTR, general time-reversible; G, gamma; I, invariant; LSU, large subunit; rDNA, ribosomal DNA.

Figure 6

Scanning electron microphotographs of Boleodorus thylactus Thorne, 1941 (female). (A–C) Anterior end in ventral and frontal views, respectively (arrows pointing to the amphidial openings). (D,E) Excretory pore in ventral and lateral views (arrow). (F, K) Lateral field at midbody showing four incisures. (G,H) Vulva in lateral and ventral views, respectively. (I) Posterior end in lateral view. (J) Anus in ventral view.
Scanning electron microphotographs of Boleodorus thylactus Thorne, 1941 (female). (A–C) Anterior end in ventral and frontal views, respectively (arrows pointing to the amphidial openings). (D,E) Excretory pore in ventral and lateral views (arrow). (F, K) Lateral field at midbody showing four incisures. (G,H) Vulva in lateral and ventral views, respectively. (I) Posterior end in lateral view. (J) Anus in ventral view.

Morphometrics of Boleodorus bushehrensis n_ sp_

CharacteristicsHolotypeParatypes
FemalesMales
n 133
L449412 ± 35.5 (334–464)432 ± 17 (417–450)
L’413379 ± 34 (298–432)399 ± 16 (385–416)
a3432.5 ± 3.0 (27.8–37.4)39.8 ± 2.5 (37.2–42.0)
b4.84.8 ± 0.5 (4–6)5.0 ± 0.5 (4.3–5.4)
c1212.6 ± 1.5 (9.3–15.8)13.1 ± 0.1 (12.9–13.0)
c’4.93.9 ± 0.5 (2.9–4.9)4.1 ± 0.1 (4.0–4.3)
V or T7974.8 ± 1.9 (70.8–79.5)
V’80.780.9 ± 1.2 (77.5–82.0)
Cephalic region height2.32.1 ± 0.3 (1.7–2.6)2.1 ± 0.4 (1.8–2.7)
Cephalic region width6.55.9 ± 0.6 (5.0–6.7)6.1 ± 0.4 (5.6–6.5)
Stylet length88.7 ± 0.4 (8.0–9.3)9.7 ± 0.3 (9.5–10.0)
Conus length33.2 ± 0.2 (3.0–3.5)3.3 ± 0.3 (3.0–3.5)
m37.536.8 ± 3.4 (32.3–42.7)33.6 ± 2.3 (31.5–36.0)
DGO33.1 ± 0.2 (2.8–3.5)3.0 ± 0.07 (2.9–3.0)
Distance from excretory pore to anterior end78.569.5 ± 5.2 (64–79)63.9 ± 1.6 (62–65)
MB48.255.8 ± 12.1 (43.0–82.3)57.8 (n=1)
Pharynx length9187 ± 7 (73–99)87.7 ± 7.5 (80–95)
Distance from anterior end to vulva333307 ± 28 (245–347)
BW1212.7 ± 1.3 (12–15)10.8 ± 0.3 (10.6–11.0)
Anal BW98.6 ± 0.6 (7–9)8.7 ± 0.9 (8–9)
Vulva–anus (V–A) distance8072.2 ± 8.5 (54–84)
PUS length11.510.6 ± 1.3 (8.2–12.0)
Tail/V–A0.50.5 ± 0.07 (0.4–0.7)
PUS/BW10.8 ± 0.07 (0.7–1.0)
Tail length3633 ± 3 (26–38)32.5 ± 0.7 (32–33)
Spicules length11.5 ± 0.4 (11.5–12.0)
Gubernaculum length4.5 ± 0.2 (4–5)
Bursa length6.3 ± 0.5 (6–7)
DOI: https://doi.org/10.2478/jofnem-2022-0004 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Oct 24, 2021
Published on: Apr 17, 2022
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2022 Somayeh Monemi, Mohammad Reza Atighi, Joaquín Abolafia, Ebrahim Pourjam, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.