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A study on the effect of the number of expansion units in a microfluidic chip on hyaluronidase-free oocyte denudation in mammals Cover

A study on the effect of the number of expansion units in a microfluidic chip on hyaluronidase-free oocyte denudation in mammals

Journal of Electrical Bioimpedance
Volume 16 (2025): Issue 1 (January 2025)
By: Ashraf Hisham Dessouky,  Haitham EL-Hussieny,  Taymour Mohammed EL-Sherry,  Victor Parque and  Ahmed M. R. Fath El-Bab  
Open Access
|Mar 2025

Figures & Tables

Fig.1:

Schematic diagram of chip A.
Schematic diagram of chip A.

Fig.2:

Detailed schematic diagram of the jagged teeth and expansion unit. (a) Jagged teeth dimensions, (b) The expansion unit dimensions.
Detailed schematic diagram of the jagged teeth and expansion unit. (a) Jagged teeth dimensions, (b) The expansion unit dimensions.

Fig.3:

Schematic diagram of chip B.
Schematic diagram of chip B.

Fig.4:

Schematic diagram of the third denudation chip
Schematic diagram of the third denudation chip

Fig.5:

The Fabricated Chips with the connected tubes. (a) Chip A. (b) Chip B. (c) Chip C.
The Fabricated Chips with the connected tubes. (a) Chip A. (b) Chip B. (c) Chip C.

Fig.6:

Different shots of the Mature oocytes with cumulus cells under the microscope.
Different shots of the Mature oocytes with cumulus cells under the microscope.

Fig.7:

Meshing analysis in Ansys.
Meshing analysis in Ansys.

Fig.8:

Shear stress at each design’s first pair of teeth at different flow rates.
Shear stress at each design’s first pair of teeth at different flow rates.

Fig.9:

The simulation results of the chips on Ansys. (a) The first channel of chip A is “1 expansion unit at 1 line design”. (b) The second channel of chip A is “19 expansion units at 1 line design”. (c) Chip B is “5 expansion units at 2-line design”. (d) Chip C is “99 expansion units at 5-line design”.
The simulation results of the chips on Ansys. (a) The first channel of chip A is “1 expansion unit at 1 line design”. (b) The second channel of chip A is “19 expansion units at 1 line design”. (c) Chip B is “5 expansion units at 2-line design”. (d) Chip C is “99 expansion units at 5-line design”.

Fig.10:

Denudation efficiency at the three chips. Where the first two channels belong to chip A, the third channel belongs to chip B, and the fourth channel belongs to chip C.
Denudation efficiency at the three chips. Where the first two channels belong to chip A, the third channel belongs to chip B, and the fourth channel belongs to chip C.

Fig.11:

Shots of the complete denudated oocytes.
Shots of the complete denudated oocytes.

Fig.12:

Shots of the incomplete denudated oocytes.
Shots of the incomplete denudated oocytes.

Fig.13:

The mechanical concept of the microfluidic chips.
The mechanical concept of the microfluidic chips.

The maturation media concentrations [62]_

ComponentFinal Concentration
TCM 1999 ml
Fetal Bovine Serum1 ml
Amphotericin B1 ml
FSH10 µl
Sodium Pyruvate20 µl
Gentamycin10 µl
Estradiol 17β10 µl
DOI: https://doi.org/10.2478/joeb-2025-0004 | Journal eISSN: 1891-5469
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Language: English
Page range: 23 - 34
Published on: Mar 20, 2025
Published by: University of Oslo
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Microfluidic,
oocyte denudation,
CO2 laser,
in vitro fertilization,
expansion units
Related subjects:
Engineering,
Bioengineering and biomedical engineering,
Biomedical electronics,
Life sciences,
Biophysics,
Medicine,
Biomedical engineering,
Physics,
Spectroscopy and metrology

© 2025 Ashraf Hisham Dessouky, Haitham EL-Hussieny, Taymour Mohammed EL-Sherry, Victor Parque, Ahmed M. R. Fath El-Bab, published by University of Oslo
This work is licensed under the Creative Commons Attribution 4.0 License.

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