Onset of oviposition by virgin queens depending on storage conditions in time between two CO2 treatments (days after treatments)
| Storage conditions* | Number of queens | Min - Max | Mean ± sd | Median |
|---|---|---|---|---|
| Nuclei | 12 | 6 - 29 | 12.5 ± 7.34 | 10 a |
| Incubator | 11 | 6 - 35 | 21.7 ± 9.35 | 17 b |
| Overall | 23 | 6 - 35 | 16.9 ± 9.43 | 14 |
Onset of oviposition by instrumentally inseminated queens and virgins (days after second CO2 treatment)
| Queens | Number of queens | Min - Max | Mean ± sd | Median |
|---|---|---|---|---|
| Inseminated | 95 | 2 - 35 | 12.0 ± 8.87 | 9a |
| Virgins | 100 | 2 - 41 | 11.7 ± 8.48 | 9a |
| Overall | 195 | 2 - 41 | 11.9 ± 8.65 | 9 |
Onset of oviposition of queens depending on time between first and second CO2 treatment (days after second treatment)
| Time between treatments | Number of queens | Min - Max | Mean ± sd | Median |
|---|---|---|---|---|
| 96 hrs | 18 | 3 - 33 | 10.3 ± 7.84 | 9a |
| 48 hrs | 22 | 2 - 26 | 7.6 ± 5.05 | 7a |
| 24 hrs | 29 | 2 - 34 | 9.9 ± 7.65 | 7a |
| 12 hrs | 19 | 2 - 30 | 10.5 ± 8.60 | 8a |
| 6 hrs | 19 | 2 - 17 | 7.8 ± 4.47 | 7a |
| 3 hrs | 22 | 2 - 41 | 10.9 ± 9.48 | 8.5a |
| 10 min* | 30 | 4 - 35 | 14.2 ± 7.98 | 11.5b |
| 5–6 sec* | 19 | 3 - 37 | 15.0 ± 7.87 | 13b |
| Control** | 17 | 6 - 41 | 21.9 ± 10.52 | 21c |
| Overall | 195 | 2 - 41 | 11.9 ± 8.65 | 9 |