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Location and Strength of Malvolio Expression in Chinese Honeybee Reveals Its Potential Role in Labor Division Cover

Location and Strength of Malvolio Expression in Chinese Honeybee Reveals Its Potential Role in Labor Division

Journal of Apicultural Science
Volume 65 (2021): Issue 1 (June 2021)
By: Weihua Ma,  Jiao Meng,  Xianyun Zhen,  Huiting Zhao,  Wanghong Li,  Ye Gao and  YuSuo Jiang  
Open Access
|Apr 2021

Figures & Tables

Fig. 1

Acmvl expression profile in age-marked worker bees. Bees were sampled at seven different time points separated by five-day intervals. Expression is relative to the value at day 30 (assigned the arbitrary value of one). Bars represent standard errors. Different letters indicate statistical differences.
Acmvl expression profile in age-marked worker bees. Bees were sampled at seven different time points separated by five-day intervals. Expression is relative to the value at day 30 (assigned the arbitrary value of one). Bars represent standard errors. Different letters indicate statistical differences.

Fig. 2

Acmvl protein production in the tissues of nurse, nectar forager and pollen forager A. c. cerana bees.A. The strip of Acmvl protein in four tissues. 1: Head (antennas included); 2: Thorax (wings included); 3: Abdomen; 4: Leg. β-actin was used as the internal control. B. The bar of Acmvl protein expression in three tissues. Image analysis was conducted using Image J. Bars represent the means of three independent experiments. Uppercase letters indicate differences between bee behavioral groups in protein production within the same tissue. Lowercase letters indicate differences between tissues within a behavioral group. Bars represent standard errors. Different letters represent statistical differences.
Acmvl protein production in the tissues of nurse, nectar forager and pollen forager A. c. cerana bees.A. The strip of Acmvl protein in four tissues. 1: Head (antennas included); 2: Thorax (wings included); 3: Abdomen; 4: Leg. β-actin was used as the internal control. B. The bar of Acmvl protein expression in three tissues. Image analysis was conducted using Image J. Bars represent the means of three independent experiments. Uppercase letters indicate differences between bee behavioral groups in protein production within the same tissue. Lowercase letters indicate differences between tissues within a behavioral group. Bars represent standard errors. Different letters represent statistical differences.

Fig. 3

Acmvl expression in pollen forager brains. Antennal lobes (AL); Kenyon cells (KC); Optic Lobes (OL).(A) Treatment group hybridized with antisense probe; dark yellow particles are positive signals. (B) Control group hybridized with the sense probe. B, C, and D depict positive signals in Kenyon cells of the mushroom body, optic lobes, and antennal lobes, respectively, while b, c, and d depict negative controls for the corresponding regions.
Acmvl expression in pollen forager brains. Antennal lobes (AL); Kenyon cells (KC); Optic Lobes (OL).(A) Treatment group hybridized with antisense probe; dark yellow particles are positive signals. (B) Control group hybridized with the sense probe. B, C, and D depict positive signals in Kenyon cells of the mushroom body, optic lobes, and antennal lobes, respectively, while b, c, and d depict negative controls for the corresponding regions.

Primer and Probe sequences of Acmvl and house gene 18S rRNA

Name for gene and probeSequence
AcmvlForward: TGCATACGTTCCTGTCATTGTGG
Reverse: AGGTGGCATATTTCTCGGTTGTG
18S rRNAForward: CCCGTAATCGGAATGAGTACACTTT
Reverse: ACGCTATTGGAGCTGGAATTACC
Acmvl anti-sense probeCAAGAGTTACGAAGCCATCTCGACAAGTTT
CGCTGCCAATTGTCGAACAAGAGGGACAGA
GATTGCCAGCTATGGGCCATCGACCGACAA
DOI: https://doi.org/10.2478/jas-2021-0004 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439
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Language: English
Page range: 61 - 70
Submitted on: Jun 7, 2019
Accepted on: Dec 31, 2020
Published on: Apr 26, 2021
Published by: Research Institute of Horticulture
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
aging,
gene expression,
location,
protein expression
Related subjects:
Life sciences,
Zoology,
Life sciences, other

© 2021 Weihua Ma, Jiao Meng, Xianyun Zhen, Huiting Zhao, Wanghong Li, Ye Gao, YuSuo Jiang, published by Research Institute of Horticulture
This work is licensed under the Creative Commons Attribution 4.0 License.

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