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Success of Selection in Terms of Hygienic Behavior in Struggle Against DWV and Varroa Cover

Success of Selection in Terms of Hygienic Behavior in Struggle Against DWV and Varroa

Journal of Apicultural Science
Volume 64 (2020): Issue 2 (December 2020)
By: Kemal Karabağ,  Ayşe Alemli and  Rahşan İvgin Tunca  
Open Access
|Dec 2020

Figures & Tables

Fig. 1

DWV loads (a) and the number of quantification cycles (b) of worker bees and pupae from control and hygienic colonies regarding qPCR. By black lines and circles, respectively represent worker bees and pupae.
DWV loads (a) and the number of quantification cycles (b) of worker bees and pupae from control and hygienic colonies regarding qPCR. By black lines and circles, respectively represent worker bees and pupae.

Supplementary Figure 1

A sample of reverse transcriptase-qPCR amplification Windows belong to DWV determined in this study. a) Amplification curves of DWV positive samples and folds. b) The standard curve is an indication of the reliability of the results. c) Heat map: Gray colored boxes show positive samples. d) Cq bars indicate quantification of DWV positive samples, negative control, and synthetic standard definite folds, respectively, from left to right.
A sample of reverse transcriptase-qPCR amplification Windows belong to DWV determined in this study. a) Amplification curves of DWV positive samples and folds. b) The standard curve is an indication of the reliability of the results. c) Heat map: Gray colored boxes show positive samples. d) Cq bars indicate quantification of DWV positive samples, negative control, and synthetic standard definite folds, respectively, from left to right.

Statistical comparison of control and hygienic colonies in terms of DWV loads, Cq values, prevalence of DWV, and Varroa loads

NMean±St.E RT-qPCRNMean±St.E Cq% PrevelancesVarroa Counts
HWorker46181627.69±64074.74**1920.10±1.15*41.328.92±5.41**
Pupa42234.38±112.342926.36±0.5569.0
CWorker50241982.35±65972.63**3317.13±0.59*66.0108.90±9.80**
Pupa46937.70±578.552825.56±0.6660.8
DOI: https://doi.org/10.2478/jas-2020-0027 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439
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Language: English
Page range: 229 - 240
Submitted on: Aug 27, 2019
Accepted on: Sep 11, 2020
Published on: Dec 18, 2020
Published by: Research Institute of Horticulture
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
deformed wing virus,
hygienic behavior,
RT-qPCR
Related subjects:
Life sciences,
Zoology,
Life sciences, other

© 2020 Kemal Karabağ, Ayşe Alemli, Rahşan İvgin Tunca, published by Research Institute of Horticulture
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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