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Histochemical Staining of Acetylcholinesterase in Carnolian Honeybee (Apis mellifera carnica) Brain after Chronic Exposure to Organophosphate Diazinon

Journal of Apicultural Science
Volume 64 (2020): Issue 1 (June 2020)
By:
Open Access
|Jul 2020

Figures & Tables

Fig. 1

AChE histochemistry in honeybee antennal lobes (A, B), mushroom body (C, D) and optic lobes (E, F) after diazinon treatment with compared to the control. al, antennal lobe; glo, glomeruli of antennal lobus; an; antennal nerve; cal, calyces; lip, lip of calyces; la, lamina; me, medulla; lo, lobula; s, somata; och, outer chiazma; ich, inner chiazma; c, c-layer; α, α-lobe of mushroom body; β, β-lobe of mushroom body; lateral protocerebrum (lp). Scales 100 µm (antennal lobus: A, B, and mushroom body: C, D) and 20µm (optic lobes: E, F).
AChE histochemistry in honeybee antennal lobes (A, B), mushroom body (C, D) and optic lobes (E, F) after diazinon treatment with compared to the control. al, antennal lobe; glo, glomeruli of antennal lobus; an; antennal nerve; cal, calyces; lip, lip of calyces; la, lamina; me, medulla; lo, lobula; s, somata; och, outer chiazma; ich, inner chiazma; c, c-layer; α, α-lobe of mushroom body; β, β-lobe of mushroom body; lateral protocerebrum (lp). Scales 100 µm (antennal lobus: A, B, and mushroom body: C, D) and 20µm (optic lobes: E, F).

Fig. 2

The effects of diazinon on AChE activity in different regions of honeybee brain. AL, antennal lobe; MB-lip, lip of calyces in mushroom body, OL-LA, lamina of optic lobes; OL-ME, medulla of optic lobes; OL-LO, lobula of optic lobes; LP-lateral protocerebrum. Data are presented as optical density (OD); mean ± standard deviation (SD). Number of bees: N=13 for control group and N=8 for diazinon-treated group;*significantly different from the control (unpaired t-test, p<0.05).
The effects of diazinon on AChE activity in different regions of honeybee brain. AL, antennal lobe; MB-lip, lip of calyces in mushroom body, OL-LA, lamina of optic lobes; OL-ME, medulla of optic lobes; OL-LO, lobula of optic lobes; LP-lateral protocerebrum. Data are presented as optical density (OD); mean ± standard deviation (SD). Number of bees: N=13 for control group and N=8 for diazinon-treated group;*significantly different from the control (unpaired t-test, p<0.05).
DOI: https://doi.org/10.2478/jas-2020-0003 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439
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Language: English
Page range: 123 - 130
Submitted on: Oct 9, 2019
Accepted on: Jan 7, 2020
Published on: Jul 2, 2020
Published by: Research Institute of Horticulture
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
acetylcholinesterase,
honeybee brain,
histochemistry,
mushroom body,
olfactory,
organophosphate
Related subjects:
Life sciences,
Zoology,
Life sciences, other

© 2020 Gordana Glavan, published by Research Institute of Horticulture
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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