Screening for antiradical efficiency of 21 semi-synthetic derivatives of quercetin in a DPPH assay

Ivana Milackova; Lucia Kovacikova; Miroslav Veverka; Ján Gallovic; Milan Stefek

doi:10.2478/intox-2013-0003

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Screening for antiradical efficiency of 21 semi-synthetic derivatives of quercetin in a DPPH assay

Interdisciplinary Toxicology

Volume 6 (2013): Issue 1 (March 2013)

By: Ivana Milackova, Lucia Kovacikova, Miroslav Veverka, Ján Gallovic and Milan Stefek

Open Access

|Jul 2013

Abstract

The group of 21 novel semi-synthetic derivatives of quercetin was screened for the antiradical efficiency in a DPPH assay. The initial fast absorbance decrease of DPPH, corresponding to the transfer of the most labile H atoms, was followed by a much slower absorbance decline representing the residual antiradical activity of the antioxidant degradation products. Initial velocity of DPPH decolorization determined for the first 75-s interval was used as a marker of the antiradical activity. Application of the kinetic parameter allowed good discrimination between the polyphenolic compounds studied. The most efficient chloronaphthoquinone derivative (compound Ia) was characterized by antiradical activity higher than that of quercetin and comparable with that of trolox. Under the experimental conditions used, one molecule of Ia was found to quench 2.6±0.1 DPPH radicals.

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Articles in this issue

DOI: https://doi.org/10.2478/intox-2013-0003 | Journal eISSN: 1337-9569 | Journal ISSN: 1337-6853

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Language: English

Page range: 13 - 17

Published on: Jul 30, 2013

Published by: Slovak Academy of Sciences, Institute of Experimental Pharmacology & Toxicology, Centre of Experimental Medicine

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

antioxidant,

quercetin derivatives,

Related subjects:

Pharmacology, toxicology

© 2013 Ivana Milackova, Lucia Kovacikova, Miroslav Veverka, Ján Gallovic, Milan Stefek, published by Slovak Academy of Sciences, Institute of Experimental Pharmacology & Toxicology, Centre of Experimental Medicine
This work is licensed under the Creative Commons License.

Volume 6 (2013): Issue 1 (March 2013)