Fasciolosis: Analytical approaches to post-treatment liver enzyme activity changes in cattle
By: Ľ. BURCÁKOVÁ, A. KÖNIGOVÁ, M. BABJÁK, Z. KOSTECKÁ and M. VÁRADY
References
- Benchaoui, H.A., Mckellar, Q.A. (1993): Effect of early treatment with rafoxanide on antipyrine clearance in sheep infected with Fasciola hepatica. Xenobiotica, 23: 439–448. DOI: 10.3109/00498259309057032
- Bosco, A., Ciuca, L., Maurelli, M.P., Vitiello, P., Cringoli, G., Prada, J.M., Rinaldi, L. (2023): Comparison of Mini-FLOTAC, Flukefinder® and sedimentation techniques for detection and quantification of Fasciola hepatica and Calicophoron daubneyi eggs using spiked and naturally infected bovine faecal samples. Parasit Vectors, 16: 260. DOI: 10.1186/s13071-023-05890-2
- Center, S.A. (2007): Interpretation of liver enzymes. Vet Clin North Am Small Anim Pract, 37: 297–333. DOI: 10.1016/j.cvsm.2006.11.009
- Coles, G.C., Bauer, C., Borgsteede, F.H.M., Geerts, S., Klei, T.R., Taylor, M.A., Waller, P.J. (1992): World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol, 44: 35–44. DOI: 10.1016/0304-4017(92)90141-U
- Coppo, J.A., Mussart, N.B., Zeinsteger, P.A. (2011): Hematological indicators of liver damage during the subclinical phase of fasciolosis in steers from Northeastern Argentina. Comp Clin Pathol, 20: 397–401. DOI: 10.1007/s00580-010-1010-9
- Corrales, J.L., McEvoy, A., Lalor, R., Cwiklinski, K., Doyle, s., Parkinson, M., Keane, O.M., Dalton, J.P., Dorey, A.L. (2023): The use of cathepsin L1 (FhCL1) serological ELISA in sentinel screening for liver fluke on sheep farms. Vet Parasitol Reg Stud Rep, 45: 100924. DOI: 10.1016/j.vprsr.2023.100924
- Costa, M., saravia, A., Ubios, D., Lores, P., Da Costa, V., Festari, M.F., Landeira, M., Rodríguez-Zraquia, s.A., Banchero, G., Freire, T. (2022): Liver function markers and haematological dynamics during acute and chronic phases of experimental Fasciola hepatica infection in cattle treated with triclabendazole. Exp Parasitol, 238: 108285. DOI: 10.1016/j.exppara.2022.108285
- Dobson, R.J., Hosking, B.C., Jacobson, C.L., Cotter, J.L., Besier, R.B., stein, P.A., Reid, s.A. (2012): Preserving new anthelmintics: a simple method for estimating faecal egg count reduction test (FECRT) confidence limits when efficacy and/or nematode aggregation is high. Vet Parasitol, 186: 79–92. DOI: 10.1016/j.vetpar.2011.11.049
- Dorchies, P. (2007): Comparison of methods for the veterinary diagnosis of liver flukes (Fasciola hepatica) in cattle. Bull Univ Agric Sci Vet Med Cluj-Napoca Vet Med, 64: 14–19. DOI: 10.15835/buasvmcn-vm:64:1–2:2270
- El-Aziem Hashem, M.A., Mohamed, s.s. (2017): Hazard assessments of cattle fascioliasis with special reference to hemato-bio-chemical biomarkers. Vet Med Open J, 2: 12–18. DOI: 10.17140/VMOJ-2-111
- Elitok, B., Elitok, Ö.M., Kabu, M. (2006): Field trial on comparative efficacy of four fasciolicides against natural liver fluke infection in cattle. Vet Parasitol, 135: 279–285. DOI: 10.1016/j.vet-par.2005.10.008
- Gedefaw, T., Mebratu, A.S., Dagnachew, S., Fenta, M.D. (2025): Comparative analysis of anthelmintic treatments: impact on liver biomarkers and clinical recovery in sheep with fasciolosis. Front Vet Sci, 12: 1485568. DOI: 10.3389/fvets.2025.1485568
- Hayward, A.D., Skuce, P.J., McNeilly, T.N. (2021): The influence of liver fluke infection on production in sheep and cattle: a meta-analysis. Int J Parasitol, 51: 913–924. DOI: 10.1016/j.ijpara.2021.02.006
- Hodžić, A., Zuko, A., Avdić, R., Alić, A., Omeragić, J., Jažić, A. (2013): Influence of Fasciola hepatica on serum biochemical parameters and vascular and biliary system of sheep liver. Iran J Parasitol, 8: 92
- Hutchinson, G.W., Dawson, K., Fitzgibbon, C.C., Martin, RJ. (2009): Efficacy of an injectable combination anthelmintic (nitroxynil+ clorsulon+ ivermectin) against early immature Fasciola hepatica compared to triclabendazole combination flukicides given orally or topically to cattle. Vet Parasitol, 162: 278–284. DOI: 10.1016/j.vetpar.2009.03.032
- Ibrahiem, H.S., Alsenosy, A.A., El-Ktany, E.M., Ata, E.B., Abas, O.M. (2023): Anthelmintic efficacy and pharmacodynamic effects of levamisole-oxyclozanide combination as (Levanide®) in fattening calves. Egypt J Vet Sci, 54: 1245–1254. DOI: 10.21608/ejvs.2023.219811.1532
- Kaneko, J.J., Harvey, J.W., Bruss, M.L. (2008): Clinical biochemistry of domestic animals. 6th Edition. Academic Press, 928 pp.
- Katsoulos, P.D., Christodoulopoulos, G., Minas, A., Pourliotis, K., Theodoropoulos, G. (2009): Use of liver enzyme activities for the diagnosis of fasciolosis in cattle. J Appl Anim Res, 36: 275–277. DOI: 10.1080/09712119.2009.9707075
- Kochapakdee, S., Pandey, V.S., Pralomkarm, W., Choldumrongkul, S., Ngampongsai, W., Lawpetchara, A. (1995): Anthelmintic resistance in goats in southern Thailand. Vet Rec, 137: 124–125. DOI: 10.1136/vr.137.5.124
- Kowalczyk, S.J., Czopowicz, M., Weber, C.N., Müller, E., Kaba, J. (2018): Accuracy of a diagnostic model based on serum biochemical parameters in detecting cows at an increased risk of chronic fascioliasis. Vet Parasitol, 254: 15–20. DOI: 10.1016/j.vetpar.2018.02.038
- Kozat, S., Denizhan, V. (2010): Glucose, lipid, and lipoprotein levels in sheep naturally infected with Fasciola hepatica. J Parasitol, 96: 657–659. DOI: 10.1645/GE-2104.1
- Königová, A., Burcáková, Ľ., Babják, M., Dolinská, M.U., Kostecká, Z., Šimková, J., Várady, M. (2024): Efficacy of a single-dose albendazole against lancet liver fluke Dicrocoelium dendriticum and liver enzymes activity in naturally infected sheep. Exp Parasitol, 256: 108656. DOI: 10.1016/j.exppara.2023.108656
- Manga-González, M.Y., Ferreras, M.C., Campo, R., González-Lanza, C., Perez, V., García-Marín, J.F. (2004): Hepatic marker enzymes, biochemical parameters and pathological effects in lambs experimentally infected with Dicrocoelium dendriticum (Digenea). Parasitol Res, 93: 344–355. DOI: 10.1007/s00436-004-1128-2
- Matanović, K., Severin, K., Martinković, F., Šimpraga, M., Janicki, Z., Barišić, J. (2007): Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica. Parasitol Res, 101: 1657–1661. DOI: 10.1007/s00436-007-0709-2
- Mehmood, K., Zhang, H., Sabir, A.J., Abbas, R.Z., Ijaz, M., Durrani, A.Z., Saleem, M.H., Rehman, M.U., Iqbal, M.K., Wang, Y., Ahmad, H.I., Abbas, T., Hussain, R., Ghori, M.T., Ali, S., Khan, A.U., Li, J. (2017): A review on epidemiology, global prevalence and economical losses of fasciolosis in ruminants. Microb Pathog, 109: 253–262. DOI: 10.1016/j.micpath.2017.06.006
- Mooney, L., Good, B., Hanrahan, J.P., Mulcahy, G., De Waal, T. (2009): The comparative efficacy of four anthelmintics against a natural acquired Fasciola hepatica infection in hill sheep flock in the west of Ireland. Vet Parasitol, 164: 201–205. DOI: 10.1016/j.vetpar.2009.05.017
- Neira, G., Sierra, R.M., Cremaschi, F., Sohaefer, N., Gonzalez, M., Godoy, D., Scarcella, S. (2024): Blood parameters and parasite burden in cattle with chronic fascioliasis. Acta Trop, 254: 107200. DOI: 10.1016/j.actatropica.2024.107200
- Persijn, J. P., van der Slik, W. (1976): A new method for the determination of γ-glutamyltransferase in serum. J Clin Chem Clin Biochem, 14: 421–428. DOI: 10.1515/cclm.1976.14.1-12.421
- Phiri, I. K., Phiri, A. M., Harrison, L. J. S. (2007): The serum glucose and β-hydroxybutyrate levels in sheep with experimental Fasciola hepatica and Fasciola gigantica infection. Vet Parasitol, 143: 287–293. DOI: 10.1016/j.vetpar.2006.09.001
- Pratt, D.S., Kaplan, M.M. (2000): Evaluation of abnormal liver-enzyme results in asymptomatic patients. N Engl J Med, 342: 1266–1271. DOI: 10.1056/NEJM200004273421707
- Raadsma, H.W., Kingsford, N.M., Spithill, T.W., Piedrafita, D. (2007): Host responses during experimental infection with Fasciola gigantica or Fasciola hepatica in Merino sheep: I. Comparative immunological and plasma biochemical changes during early infection. Vet Parasitol, 143: 275–286. DOI: 10.1016/j.vet-par.2006.09.008
- Reitman, S., Frankel, S. (1957): A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol, 28: 56–63. DOI: 10.1093/ajcp/28.1.56
- Shrimali, R.G., Patel, M.D., Patel, R.M. (2016): Comparative efficacy of anthelmintics and their effects on hemato-biochemical changes in fasciolosis of goats of South Gujarat. Vet World, 9: 524. DOI: 10.14202/vetworld.2016.524-529
- Schumann, G., Canalias, F., Joergensen, P.J., Kang, D., Lessinger, J.M., Klauke, R. (2010): IFCC reference procedures for measurement of the catalytic concentrations of enzymes: Corrigendum, notes and useful advice: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)-IFCC Scientific Division. Clin Chem Lab Med, 48: 615–621. DOI: 10.1515/CCLM.2010.137
- Schumann, G., Klauke, R., Canalias, F., Bossert-Reuther, S., Franck, P.F.H., Gella, F.J., Jørgensen, P.J., Kang, D., Lessinger, J.M., Panteghini, M., Ceriotti, F. (2011): IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37° C. Part 9: Reference procedure for the measurement of catalytic concentration of alkaline phosphatase: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Scientific Division, Committee on Reference Systems of Enzymes (C-RSE) 1. Clin Chem Lab Med, 49: 1439–1446. DOI: 10.1515/CCLM.2011.621
- Wang, W., Dong, Z., Zhang, J., Zhou, X., Wei, X., Cheng, F., Zhang, J. (2019): Acute and subacute toxicity assessment of oxyclozanide in Wistar rats. Front Vet Sci, 6: 294. DOI: 10.3389/fvets.2019.00294
- Yang, Q., Mao, W.H., Ferre, I., Bayón, J.E., Mao, X.Z., González-Gallego, J. (1998): Plasma aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), and gamma-glutamyl transpeptidase (GGT) activities in water buffaloes with experimental subclinical fasciolosis. Vet Parasitol, 78: 129–136. DOI: 10.1016/S0304-4017(98)00134-4
- Zaitsev, S.Y., Bogolyubova, N.V., Zhang, X., Brenig, B. (2020): Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis: A review. PeerJ, 8: e8997. DOI: 10.7717/peerj.8997
Language: English
Page range: 79 - 86
Submitted on: Jan 24, 2026
Accepted on: Mar 4, 2026
Published on: Jun 15, 2026
Published by: Slovak Academy of Sciences, Institute of Parasitology
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open
Keywords:
Related subjects:
© 2026 Ľ. BURCÁKOVÁ, A. KÖNIGOVÁ, M. BABJÁK, Z. KOSTECKÁ, M. VÁRADY, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution 4.0 License.