Calculations of percentages of after treatment enzymes change with level of significance analyzed by Kruskal-Wallis test: NS – not significant, * – p < 0_05; ** – p < 0_01; *** – p < 0_001_
| Enzyme | Group | Day | Formula 1 (%) | EC | Formula 2 (%) | EC | Formula 3 (%) | EC |
|---|---|---|---|---|---|---|---|---|
| ABZ | 7 | 9.7NS | I | 10.7NS | I | 47.8*** | I | |
| 21 | 10.5NS | I | 11.7NS | I | 61.1*** | I | ||
| AST | LEV+ | 7 | 10.9* | I | 12.2* | I | 46.0*** | I |
| OXY | 21 | 10.9* | I | 12.2* | I | 57.9*** | I | |
| IVM+ | 7 | 3.1NS | I | 3.2 NS | I | 58.6** | I | |
| CLOR | 21 | 10.5 NS | I | 11.7 NS | I | 85.5*** | I | |
| ABZ | 7 | 24.1*** | D | 19.5*** | D | 10.8 NS | I | |
| 21 | 60.7*** | D | 37.8*** | D | 34.6 NS | D | ||
| ALT | LEV+ | 7 | 27.3*** | D | 21.5*** | D | 31.3 NS | D |
| OXY | 21 | 9.1*** | D | 8.3*** | D | 38.7* | D | |
| IVM+ | 7 | 37.6 NS | I | 60.4 NS | I | 86.7* | I | |
| CLOR | 21 | 67.2*** | D | 40.2*** | D | 46.8*** | D | |
| ABZ | 7 | 28.4 NS | I | 39.6 NS | I | 24.1 NS | I | |
| 21 | 28.6 NS | I | 40.0 NS | I | 55.6 NS | I | ||
| ALP | LEV+ | 7 | 18.6 NS | I | 22.9 NS | I | 13.5 NS | I |
| OXY | 21 | 1.7 NS | D | 1.7 NS | D | 13.5 NS | I | |
| IVM+ | 7 | 11.1 NS | I | 12.5 NS | I | 25.6 NS | I | |
| CLOR | 21 | 21.9 NS | D | 17.9 NS | D | 14.5 NS | I | |
| ABZ | 7 | 27.1*** | D | 21.3*** | D | 19.8* | D | |
| 21 | 47.5*** | D | 32.2*** | D | 21.6** | D | ||
| GGT | LEV+ | 7 | 13.0*** | D | 11.5*** | D | 32.5* | D |
| OXY | 21 | 34.1*** | D | 25.4*** | D | 35.3** | D | |
| IVM+ | 7 | 4.0*** | D | 3.8*** | D | 7.4 NS | D | |
| CLOR | 21 | 31.1*** | D | 23.7*** | D | 16.7 NS | D |
Anthelmintic schedule for control and treatment groups_
| Group | Control | IVM+CLOR* | LEV+OXY* | ABZ* |
|---|---|---|---|---|
| Trade name | - | Ivomec Super Injection® | Interzan Gold Oral® | Albendavet 10%® |
| Effective ingredient | - | ivermectin, clorsulon | levamisole, oxyclozanide | albendazole |
| Dosing | - | 1 ml per 50 kg b.wt., parenteral | 2.5 ml per 10 kg b.wt., peroral | 1 ml per 10 kg b.wt., peroral |
Comparisons between the calculations of enzyme activity changes using Formula 1,2 and 3 analyzed by Kruskal-Wallis test: NS – not significant, level of significance at p < 0_05_
| AST | ALT | ALP | GGT | ||
|---|---|---|---|---|---|
| increase | Formula 1 / Formula 2 | NS | NS | p<0.05 | NS |
| Formula 1 / Formula 3 | p<0.001 | p<0.001 | NS | NS | |
| Formula 2 / Formula 3 | p<0.001 | p<0.05 | p<0.001 | NS | |
| decrease | Formula 1 / Formula 2 | NS | p<0.05 | p<0.01 | p<0.01 |
| Formula 1 / Formula 3 | NS | p<0.05 | p<0.001 | p<0.05 | |
| Formula 2 / Formula 3 | NS | NS | NS | NS |
The average individual calculations of the enzyme activity change using Formula 1 (F1), 2 (F2) and 3 (F3) showing different numbers of animals (n) with increased and decreased activity on Day 7 and 21_
| Enzyme | Day | Increased enzyme activity (%) | Decreased enzyme activity (%) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| F1 | n1 | F2 | n2 | F3 | n3 | F1 | n1 | F2 | n2 | F3 | n3 | ||
| AST | 7 | 11.6 | 8 | 15.0 | 8 | 61.0 | 34 | 16.7 | 28 | 13.4 | 28 | 23.2 | 2 |
| 21 | 4.4 | 4 | 4.7 | 4 | 72.5 | 36 | 13.4 | 32 | 11.3 | 32 | 0 | 0 | |
| ALT | 7 | 28.3 | 21 | 50.0 | 21 | 62.5 | 23 | 66.2 | 15 | 33.2 | 15 | 28.2 | 13 |
| 21 | 17.7 | 9 | 25.3 | 9 | 0 | 0 | 62.9 | 27 | 33.1 | 27 | 36.4 | 36 | |
| ALP | 7 | 50.0 | 17 | >100 | 17 | 55.0 | 24 | >100 | 19 | 50.1 | 19 | 35.8 | 12 |
| 21 | 46.8 | 15 | >100 | 15 | 39.3 | 27 | >100 | 21 | 51.3 | 21 | 32.8 | 9 | |
| GGT | 7 | 13.4 | 10 | 17.7 | 10 | 15.8 | 10 | 47.8 | 26 | 28.3 | 26 | 32.9 | 26 |
| 21 | 12.2 | 8 | 14.3 | 8 | 24.7 | 5 | 54.0 | 28 | 31.0 | 28 | 31.6 | 31 | |