Entomopathogenic nematodes targeting the control of the corn leafhopper, Dalbulus maidis (Hemiptera: Cicadellidae), under laboratory conditions

A. C. DE OLIVEIRA; V. ANDALÓ; M. L. DA MOTA; F. J. CARVALHO; L. S. DE FARIA; O. J. MARQUES

doi:10.2478/helm-2026-0003

.blurhash-client-img { display: none !important; }

Entomopathogenic nematodes targeting the control of the corn leafhopper, Dalbulus maidis (Hemiptera: Cicadellidae), under laboratory conditions

Helminthologia

Volume 63 (2026): Issue 1 (March 2026)

By: A. C. DE OLIVEIRA , V. ANDALÓ , M. L. DA MOTA , F. J. CARVALHO , L. S. DE FARIA and O. J. MARQUES

Open Access

|Apr 2026

Figures & Tables

Adult Dalbulus maidis killed by entomopathogenic nematodes under laboratory conditions. A. Heterorhabditis amazonensis MC01. B. Steinernema feltiae IBCB47.

Lethal concentration of entomopathogenic required to kill Dalbulus maidis adults under laboratory conditions. A. Heterorhabditis amazonensis MC01. B. Steinernema feltiae IBCB47.

Compatibility of Steinernema feltiae IBCB 47 with phytosanitary products after 48 hours of contact_

Treatment	Viability(%)*	Infectivity(%)*	MC(%)	Rfin(%)	Rfec(%)	E(%)	IOBCClassification
Chlorfluazuron	92.71 ± 0.95 a	72.50 ± 9.18b	80.24	2.48	53.20	83.18	ModeratelyHarmful
Atrazine	88.43 ± 1.80c	72.50 ± 7.29b	6.88	27.50	0.00	34.38	SlightlyHarmful
Methomyl	97.18 ± 0.72 a	65.00 ± 13.92b	0.00	35.00	43.17	75.93	SlightlyHarmful
Chlorpyrifos	95.46± 0.67 a	80.00 ± 7.29b	0.00	20.00	67.55	87.07	ModeratelyHarmful
Profenofos + Lufenuron	91.74 ± 1.19b	65.00 ± 2.45b	3.48	35.00	0.20	38.67	SlightlyHarmful
Deltamethrin	86.15 ± 2.81c	97.50 ± 2.50 a	9.42	2.50	37.81	49.73	SlightlyHarmful
Glyphosate	96.44 ± 1.06 a	70.00 ± 10.89b	0.00	30.00	11.71	40.24	ModeratelyHarmful
Tembotrione	97.11 ± 1.26 a	87.50 ± 9.68 a	0.00	12.50	8.37	18.67	Harmless
2,4D	95.79 ± 1.39 a	60.00 ± 7.29b	0.00	40.00	7.82	47.04	SlightlyHarmful
Control	95.07 ± 0.97 a	100.00 ± 0.00a	0.00	0.00	0.00	0.00	Harmless
CV (%)	3.38	24.24

Duration of developmental stages of Heterorhabditis amazonensis MC01 in Dalbulus maidis at a concentration of 5 infective juveniles per insect (long cycle)_

Developmental stage	Assessment time (h)
	72	144	168	216	264
J₄	0 - 10	10	6
Hermaphrodites + eggs/J₁			3
Hermaphrodites + J₂
J₃ and J₄
Females and Males		1	31
Females + eggs/J₁				50	300
Females + J₂
J₃/JI		2
J₄ (2nd Generation)

Duration of developmental stages of Steinernema feltiae IBCB 47 in Dalbulus maidis at a concentration of 100 infective juveniles per insect (short cycle)_

Developmental stage	Assessment time (h)
	96	120	144	192	216	240	264	288
J₄	92	68	137	4
Hermaphrodites + eggs/J₁	0 - 10	4	8		4
Hermaphrodites + J₂				17
J₃ and J₄						15	6
Females and Males						15	4
Females + eggs/J₁
Females + J₂								31
J₃/JI							13	16
J₄ (2nd Generation)								8

Duration of developmental stages of Heterorhabditis amazonensis MC01 in Dalbulus maidis at a concentration of 100 infective juveniles per insect (short cycle)_

Developmental stage	Assessment time (h)
	72	96	120	144	192	216	240	264
J₄	0 - 10	10	18	3	10
Hermaphrodites + eggs/J₁				56
Hermaphrodites + J₂						18
J₃ and J₄			50
Females and Males				1	31
Females + eggs/J₁							279	755
Females + J₂
J₃/JI				2
J₄ (2nd Generation)

Mortality (%) of Dalbulus maidis adults caused by different isolates of entomopathogenic nematodes under laboratory conditions_

Treatment	Mortality (%)*
Heterorhabditis amazonensis GL	66.48 ± 4.20 a
Heterorhabditis amazonensis MC01	62.61 ± 13.71 a
Heterorhabditis amazonensis UENP2	78.68 ± 6.16 a
Heterorhabditis amazonensis UENP6	65.53 ± 7.29 a
Steinernema brazilense IBCB 06	52.85 ± 9.07 a
Steinernema carpocapsae IBCB 02	51.48 ± 6.64 a
Steinernema feltiae IBCB 47	77.62 ± 5.31 a
CV (%)	10.83

Compatibility of Heterorhabditis amazonensis MC01 with phytosanitary products after 48 hours of contact_

Treatment	Viability(%)*	Infectivity(%)*	MC	Rfin(%)	Rfec(%)	E(%)	IOBCClassification
Chlorfluazuron	68.85 ± 2.62c	35.00 ± 7.29b	24.28	51.07	2.33	77.68	SlightlyHarmful
Atrazine	77.75 ± 2.03b	62.50 ± 12.50 a	14.53	30.36	0.00	44.88	SlightlyHarmful
Methomyl	69.90 ± 1.87c	72.50 ± 9.18 a	23.16	13.92	19.56	56.64	SlightlyHarmful
Chlorpyrifos	66.17 ± 3.63c	30.00 ± 8.48b	27.28	63.57	42.65	133.51	Harmful
Profenofos + Lufenuron	60.50 ± 3.27c	49.00 ± 9.00 b	33.52	50.00	13.36	130.59	Harmful
Deltamethrin	62.10 ± 4.53c	62.50 ± 12.50 a	31.73	24.52	37.30	93.55	ModeratelyHarmful
Glyphosate	77.54 ± 3.50 b	30.00 ± 10.23b	14.81	58.09	5.14	78.04	SlightlyHarmful
Tembotrione	79.47 ± 2.71 b	70.00 ± 14.49 a	12.60	25.83	14.56	52.60	SlightlyHarmful
2.4D	80.85 ± 2.90b	32.50 ± 12.25 b	11.12	57.62	11.09	79.83	ModeratelyHarmful
Control	90.97 ± 0.42 a	77.50 ± 8.29 a	0.00	0.00	0.00	0.00	Harmless
CV (%)	14.76	17.69

Duration of developmental stages of Steinernema feltiae IBCB 47 in Dalbulus maidis at a concentration of 5 infective juveniles per insect (long cycle)_

Developmental stage	Assessment time (h)
	72	144	168	216	240	264	288
J₄	0 - 10	3
Hermaphrodites + eggs/J₁	0 - 5	2	3
Hermaphrodites + J₂			3	3
J₃ and J₄			1
Females and Males					7
Females + eggs/J₁					8
Females + J₂
J₃/JI							9
J₄ (2nd Generation)						2	4

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.2478/helm-2026-0003 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605

Journal RSS Feed

Language: English

Page range: 29 - 37

Submitted on: Feb 19, 2025

Accepted on: Feb 23, 2026

Published on: Apr 27, 2026

Published by: Slovak Academy of Sciences, Institute of Parasitology

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

Viruses,

Related subjects:

Zoology,

Ecology,

Life sciences, other,

Medicine,

Clinical medicine,

Microbiology, virology and infection epidemiology

© 2026 A. C. DE OLIVEIRA, V. ANDALÓ, M. L. DA MOTA, F. J. CARVALHO, L. S. DE FARIA, O. J. MARQUES, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 63 (2026): Issue 1 (March 2026)

Entomopathogenic nematodes targeting the control of the corn leafhopper, Dalbulus maidis (Hemiptera: Cicadellidae), under laboratory conditions

Figures & Tables

Fig 1.

Fig. 2.

Compatibility of Steinernema feltiae IBCB 47 with phytosanitary products after 48 hours of contact_

Duration of developmental stages of Heterorhabditis amazonensis MC01 in Dalbulus maidis at a concentration of 5 infective juveniles per insect (long cycle)_

Duration of developmental stages of Steinernema feltiae IBCB 47 in Dalbulus maidis at a concentration of 100 infective juveniles per insect (short cycle)_

Duration of developmental stages of Heterorhabditis amazonensis MC01 in Dalbulus maidis at a concentration of 100 infective juveniles per insect (short cycle)_

Mortality (%) of Dalbulus maidis adults caused by different isolates of entomopathogenic nematodes under laboratory conditions_

Compatibility of Heterorhabditis amazonensis MC01 with phytosanitary products after 48 hours of contact_

Duration of developmental stages of Steinernema feltiae IBCB 47 in Dalbulus maidis at a concentration of 5 infective juveniles per insect (long cycle)_

Paradigm

My account