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Fig. 5.
Diversity indices and neutrality tests values of S_ chrysophrii samples collected from Tunisian sites based on COI marker_
| Population | n | S | h | k | Pi | Hd | Tajima's D | Fu's Fs |
|---|---|---|---|---|---|---|---|---|
| Ghar El Melh (C) NORTH | 20 | 5 | 8 | 1.336 | 0.00440 | 0.815 | −0.15742 | −3.745 |
| Tboulba CENTER | 10 | 5 | 6 | 1.466 | 0.00482 | 0.888 | −0.68235 | −2.691 |
| Mehdia CENTER | 11 | 4 | 5 | 0.981 | 0.00323 | 0.709 | −1.02918 | −2.111 |
| Bizerte NORTH | 11 | 11 | 5 | 2.836 | 0.00933 | 0.781 | −1.05320 | 0.425 |
| Djerba SOUTH | 17 | 4 | 7 | 1.308 | 0.00431 | 0.830 | 0.32498 | −2.954 |
| Sfax SOUTH | 10 | 3 | 5 | 1.177 | 0.00387 | 0.755 | 0.39804 | −1.849 |
| Ghar El Melh (W) NORTH | 9 | 4 | 5 | 1.777 | 0.00585 | 0.861 | 0.84519 | −1.113 |
Analysis of Molecular Variance (AMOVA) of Sparicotyle chrysophrii samples based on their hosts origin, grouped into two groups (wild versus cage-reared Tunisian hosts)_
| Source of variation | df | Sum of squares | Variance components | % variation | P-value |
|---|---|---|---|---|---|
| Among groups | 1 | 1.603 | 0.03496 Va | 4.92 | *** |
| Among populations within groups | 5 | 5.911 | 0.04239 Vb | 5.96 | *** |
| Within populations | 81 | 51.327 | 0.63367 Vc | 89.12 | *** |
| Total | 87 | 58.841 | 0.71102 | ||
Genetic diversity indices and neutrality tests values of S_ chrysophrii populations from the Mediterranean Sea based on COI marker_
| Population | n | S | h | k | Pi | Hd | Tajima's D | Fu's Fs |
|---|---|---|---|---|---|---|---|---|
| Tunisia | 88 | 12 | 15 | 1.56818 | 0.00519 | 0.82941 | −0.91998 | −6.945 |
| Adriatic Sea | 9 | 16 | 5 | 3.88889 | 0.01288 | 0.80556 | −1.64543 | 0.624 |
| Algeria | 5 | 13 | 5 | 5.40000 | 0.01788 | 1.00000 | −0.97762 | −1.223 |
| Spain | 22 | 16 | 15 | 4.61039 | 0.01527 | 0.96104 | 0.18310 | −6.121 |
| Italy | 23 | 16 | 11 | 3.81028 | 0.01262 | 0.85771 | −0.43524 | −2.204 |
Analysis of Molecular Variance (AMOVA) of Sparicotyle chrysophrii samples from the North and the South Mediterranean Sea (North versus South Mediterranean Sea)_
| Source of variation | df | Sum of squares | Variance components | % variation | P-value |
|---|---|---|---|---|---|
| Among groups | 1 | 192.471 | 2.39800 Va | 55.54 | *** |
| Among populations within groups | 2 | 10.640 | 0.29939 Va | 6.93 | *** |
| Within populations | 148 | 239.751 | 1.61994 Va | 37.52 | *** |
| Total | 151 | 442.862 | 4.31733 | ||
List of Sparicotyle chrysophrii specimens collected from the gills of wild and farmed Sparus aurata in Tunisia_
| S. aurata | Locality (Code) | NHE | NHP | P (MI) | n-28S | n-COI |
|---|---|---|---|---|---|---|
| Wild | Bizerte (BZR) NORTH | 11 | 7 | 63.6 % (1.7) | 1 | 11 |
| Ghar El Meleh (GHS) NORTH | 13 | 5 | 38.5 % (2.0) | 1 | 9 | |
| Sfax (SFX) SOUTH | 16 | 6 | 37.5 % (1.7) | 1 | 10 | |
| Cage-reared | Ghar El Melh (GHE) NORTH | 10 | 9 | 90.0 % (2.4) | 1 | 20 |
| Tboulba (TBO) CENTER | 17 | 5 | 29.4 % (2.8) | 1 | 10 | |
| Mehdia (MEH) CENTER | 15 | 7 | 46.7 % (1.6) | 1 | 11 | |
| Djerba (DJE) SOUTH | 15 | 5 | 33.4 % (3.4) | 1 | 17 | |
Analysis of the genetic diversity of Sparicotyle chrysophrii populations from the Mediterranean Sea (Pairwise FST between populations (lower diagonal), nucleotide divergence (Dxy)/the net genetic distance (Da) between populations)_
| Tunisia | Algeria | Spain | Italy | Adriatic Sea | |
|---|---|---|---|---|---|
| Tunisia | - | 0.01148/-0.00006 | 0.02961/0.01938 | 0.03171/0.02281 | 0.03639/0.02735 |
| Algeria | −0.00525 | - | 0.03188/0.0153 | 0.0334/0.01815 | 0.03797/0.02259 |
| Spain | 0.65457 | 0.4801 | - | 0.01935/0.00541 | 0.01945/0.00538 |
| Italy | 0.71922 | 0.54346 | 0.27953 | - | 0.01416/0.00141 |
| Adriatic Sea | 0.75169 | 0.59496 | 0.2765 | 0.09959 | - |
Analysis of Molecular Variance (AMOVA) of Sparicotyle chrysophrii samples from Tunisia, divided into three groups (North, Central and South)_
| Source of variation | df | Sum of squares | Variance components | % variation | P-value |
|---|---|---|---|---|---|
| Among groups | 2 | 2.479 | −0.00269 Va | −0.39 | *** |
| Among populations within groups | 4 | 5.034 | 0.05206 Vb | 7.62 | *** |
| Within populations | 81 | 51.327 | 0.63367 Vc | 92.77 | *** |
| Total | 87 | 58.841 | 0.68303 | ||