In vitro protoscolicidal effects of Hypericum perforatum, Thymus vulgaris, Pimenta racemosa, and Mentha piperita against Echinococcus granulosus protoscoleces

In vitro protoscolicidal effects of Hypericum perforatum, Thymus vulgaris, Pimenta racemosa, and Mentha piperita against Echinococcus granulosus protoscoleces

Helminthologia

Volume 62 (2025): Issue 2 (June 2025)

By:

R. Yildiz and A. H. Unlu

Open Access

|Sep 2025

References

Adas, G., Arikan, S., Kemik, O., Oner, A., Sahip, N., Karatepe, O. (2009): Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World J Gastroenterol, 15(1): 112. DOI: <a href="https://doi.org/10.3748/wjg.15.112" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.3748/wjg.15.112</a>
Open DOI Search in Google Scholar Back to article
Al-Khlifeh, E., Saidat, N., Khleifat, K., Al Qaisi, Y. (2023): Phytochemical profile and in vitro protoscolicidal effects of Juniperus phoenicea L., Calotropis procera (Aiton) Dryand, and Artemisia judaica L. against Echinococcus granulosus cysts. J. Pharm. Pharmacogn. Res., 11(4): 635-650. DOI: <a href="https://doi.org/10.56499/jppres23.1635_11.4.635" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.56499/jppres23.1635_11.4.635</a>
Open DOI Search in Google Scholar Back to article
Alvi, M.A., Khan, S., Ali, R.M.A., Qamar, W., Saqip, M., Faridi, N.Y., Jia, W.Z. (2022): Herbal medicines against hydatid disease: A systematic review (2000–2021). Life, 12(5): 676. DOI: <a href="https://doi.org/10.3390/life12050676" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.3390/life12050676</a>
Open DOI Search in Google Scholar Back to article
Amarowicz, R., Żegarska, Z., Rafałowski, R., Pegg, R.B., Karamać, M., Kosińska, A. (2009): Antioxidant activity and free radical-scavenging capacity of ethanolic extracts of thyme, oregano, and marjoram. Eur J Lipid Sci Technol, 111(11): 1111-1117. DOI: <a href="https://doi.org/10.1002/ejlt.200800170" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1002/ejlt.200800170</a>
Open DOI Search in Google Scholar Back to article
Amri, M., Touil-Boukoffa, C. (2016): In vitro anti-hydatic and immunomodulatory effects of ginger and [6]-gingerol. Asian Pac J Trop Med, 9(8): 749-756. DOI: <a href="https://doi.org/10.1016/j.apjtm.2016.06.013" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1016/j.apjtm.2016.06.013</a>
Open DOI Search in Google Scholar Back to article
Benmarce, M., Haif, A., Elissondo, M. C., Bouaziz, S., Bentahar, A., Laatamna, A. (2024): Comparison of the scolicidal activity of two leaves extracts of Myrtus communis from Algeria against Echinococcus granulosus sensu lato protoscoleces. Acta Parasitol, 1-15. DOI: <a href="https://doi.org/10.1007/s11686-024-00808-z" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1007/s11686-024-00808-z</a>
Open DOI Search in Google Scholar Back to article
Bekele, J., Butako, B. (2011): Occurrence and financial loss assessment of cystic echinococcosis (hydatidosis) in cattle slaughtered at Wolayita Sodo municipal abattoir, Southern Ethiopia. Trop Anim Health Prod, 43: 221–228. DOI: <a href="https://doi.org/10.1007/s11250-010-9680-5" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1007/s11250-010-9680-5</a>
Open DOI Search in Google Scholar Back to article
Boakye, Y.D., Adjei, D.K., Yeboah, K.O., Mensah, D.O., Osafo, N., Agana, T.A., Agyare, C. (2023): Natural Products as Therapeutic Option for Echinococcosis. In: Inceboz, T., Rodriguez-Morales, A.J. (Eds) Echinococcosis - New Perspectives. IntechOpen, pp. 1–26. DOI: <a href="https://doi.org/10.5772/intechopen.109614" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.5772/intechopen.109614</a>
Open DOI Search in Google Scholar Back to article
Bouaziz, S., Amri, M., Taibi, N., Zeghir-Bouteldja, R., Benkhaled, A., Mezioug, D., Touil-Boukoffa, C. (2021): Protoscolicidal activity of Atriplex halimus leaves extract against Echinococcus granulosus protoscoleces. Exp Parasitol, 229: 108155. DOI: <a href="https://doi.org/10.1016/j.exppara.2021.108155" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1016/j.exppara.2021.108155</a>
Open DOI Search in Google Scholar Back to article
Budke, C.M., White, A.C., Jr, Garcia, H.H. (2009): Zoonotic larval cestode infections: neglected, neglected tropical diseases? PLoS Negl Trop Dis, 3(2): e319. DOI: <a href="https://doi.org/10.1371/journal.pntd.0000319" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1371/journal.pntd.0000319</a>
Open DOI Search in Google Scholar Back to article
Camele, I., Grul’ová, D., Elshafie, H. S. (2021): Chemical Composition and Antimicrobial Properties of Mentha × piperita cv. ‘Kristinka’ Essential Oil. Plants, 10(8): 1567. DOI: <a href="https://doi.org/10.3390/plants10081567" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.3390/plants10081567</a>
Open DOI Search in Google Scholar Back to article
Chen, H., Muhammad, I., Zhang, Y., Ren, Y., Zhang, R., Huang, X., Diao, L., Liu, H., Li, X., Sun, X., Abbas, G., Li, G. (2019): Antiviral activity against infectious bronchitis virus and bioactive components of Hypericum perforatum L. Front Pharmacol, 10: 1272. DOI: <a href="https://doi.org/10.3389/fphar.2019.01272" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.3389/fphar.2019.01272</a>
Open DOI Search in Google Scholar Back to article
Deberdt, P., Davezies, I., Coranson-Beaudu, R., Jestin, A. (2018): Efficacy of Leaf Oil from Pimenta racemosa var. racemosa in Controlling Bacterial Wilt of Tomato. Plant Dis, 102(1): 124–131. DOI: <a href="https://doi.org/10.1094/PDIS-04-17-0593-RE" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1094/PDIS-04-17-0593-RE</a>
Open DOI Search in Google Scholar Back to article
El-Desouky, T.A. (2021): Evaluation of effectiveness aqueous extract for some leaves of wild edible plants in Egypt as anti-fungal and anti-toxigenic. Heliyon, 7(2): e06209. DOI: <a href="https://doi.org/10.1016/j.heliyon.2021.e06209" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1016/j.heliyon.2021.e06209</a>
Open DOI Search in Google Scholar Back to article
ElShaarawy, F.S., Abdelhady, M.I., Hamdy, W., Ibrahim, H.A. (2024): Investigation of the Essential Oil Constituents of Pimenta racemosa Aerial Parts and Evaluation of Its Antiviral Activity against Hsv 1 and 2. Trends Adv. Sci. Technol., 1(1), 2. DOI: <a href="https://doi.org/10.62537/2974-444X.1006" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.62537/2974-444X.1006</a>
Open DOI Search in Google Scholar Back to article
Gavarić, N., Kovač, J., Kretschmer, N., Kladar, N., Možina, S. S., Bucar, f., Bauer, R., Božin, B. (2015): Natural products as antibacterial agents—antibacterial potential and safety of post-distillation and waste material from Thymus vulgaris L., Lamiaceae. In: Concepts, Compounds and the Alternatives of Antibacterials. InTech. 123-151. DOI: <a href="https://doi.org/10.5772/60869" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.5772/60869</a>
Open DOI Search in Google Scholar Back to article
Hernández-Saavedra, D., Pérez-Ramírez, I.F., Ramos-gómez, M., Mendoza-Díaz, S., Loarca-Pina, G., Reynoso-Camacho, R. (2016): Phytochemical characterization and effect of Calendula officinalis, Hypericum perforatum, and Salvia officinalis infusions on obesity-associated cardiovascular risk. Med Chem Res, 25: 163-172. DOI: <a href="https://doi.org/10.1007/s00044-015-1454-1" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1007/s00044-015-1454-1</a>
Open DOI Search in Google Scholar Back to article
Hudz, N., Kobylinska, L., Pokajewicz, K., Horčinová SedláčKová, V., fedin, R., Voloshyn, M., Myskiv, I., BrindzA, J., Wieczorek, P.P., Lipok, J. (2023): Mentha piperita: Essential oil and extracts, their biological activities, and perspectives on the development of new medicinal and cosmetic products. Molecules, 28(21): 7444. DOI: <a href="https://doi.org/10.3390/molecules28217444" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.3390/molecules28217444</a>
Open DOI Search in Google Scholar Back to article
Jamroz, D., Orda, J., Kamel, C., Wiliczkiewicz, A., Wertelecki, T., SkorupińSka, J. (2003): The influence of phytogenic extracts on performance, nutrient digestibility, carcass characteristics, and gut microbial status in broiler chickens. J Anim Feed Sci, 12(3): 583596. DOI: <a href="https://doi.org/10.22358/jafs/67752/2003" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.22358/jafs/67752/2003</a>
Open DOI Search in Google Scholar Back to article
Jairus, M., Jeff, C., Jeffrey, C., Marvelous, S. (2023): Occurrence and economic impact of cystic echinococcosis in cattle slaughtered in the Matabeleland Region, Zimbabwe. Acta Trop, 242: 106918. DOI: <a href="https://doi.org/10.1016/j.actatropica.2023.106918" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1016/j.actatropica.2023.106918</a>
Open DOI Search in Google Scholar Back to article
Jasim, A.H. (2023): The Effect of Alcoholic and Aqueous Extracts of Malva Sylvestris L. on the Vitality of protoscolices in Echinococcus granulosus (In Vitro and In Vivo). J Commun Dis, 55(3B): 40-47. DOI: <a href="https://doi.org/10.24321/0019.5138.202336" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.24321/0019.5138.202336</a>
Open DOI Search in Google Scholar Back to article
Kheirandish, F., Marzban, A., Masoori, L., Nourmohammadi, M., Mo-Radpour, K., Cheraghipour, K. (2020): Efficacy of thymol on the scolices of hydatid cyst. Herb Med J, 5 (1): 37-44. DOI: <a href="https://doi.org/10.22087/hmj.v5i1.786" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.22087/hmj.v5i1.786</a>
Open DOI Search in Google Scholar Back to article
Kladar, N., MrđAnović, J., AnačKov, G., Šolajić, S., Gavarić, N., SrđEnović, B., Božin, B. (2017): Hypericum perforatum: Synthesis of Active Principles during Flowering and Fruitification-Novel Aspects of Biological Potential. Evid Based Complement Alternat Med, 2017: 2865610. DOI: <a href="https://doi.org/10.1155/2017/2865610" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1155/2017/2865610</a>
Open DOI Search in Google Scholar Back to article
Kohansal, M.H., Nourian, A., Rahimi, M.T., Daryani, A., Spotin, A., Ahmadpour, E. (2017): Natural products applied against hydatid cyst protoscolices: A review of past to present. Acta Trop, 176: 385-394. DOI: <a href="https://doi.org/10.1016/j.actatropica.2017.09.013" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1016/j.actatropica.2017.09.013</a>
Open DOI Search in Google Scholar Back to article
Labsi, M., Khelifi, L., Mezioug, D., Soufli, i., Touil-Boukoffa, C. (2016): Antihydatic and immunomodulatory effects of Punica granatum peel aqueous extract in a murine model of echinococcosiS. Asian Pac J Trop Dis, 9(3): 211-220. DOI: <a href="https://doi.org/10.1016/j.ap-jtM.2016.01.038" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1016/j.ap-jtM.2016.01.038</a>
Open DOI Search in Google Scholar Back to article
Labsi, M., Soufli, I., Khelifi, L., Amir, Z.C., Touil-Boukoffa, C. (2019): A preventive effect of the combination of albendazole and pomegranate peel aqueous extract treatment in cystic echinococcosis mice model: An alternative approach. Acta Trop, 197: 105050. DOI: <a href="https://doi.org/10.1016/j.actatropica.2019.105050" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1016/j.actatropica.2019.105050</a>
Open DOI Search in Google Scholar Back to article
Maggiore, M.A., Albanese, A.A., Gende, L.B., Eguaras, M.J., Denegri, G.M., Elissondo, M.C. (2012): Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodeS. Parasitol Res, 110: 1103-1112. DOI: <a href="https://doi.org/10.1007/s00436-011-2595-x" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1007/s00436-011-2595-x</a>
Open DOI Search in Google Scholar Back to article
Mohammed, N.R., Dalar, A., Ozdemir, F.A., Turker, M. (2019): In vitro propagation and secondary metabolites investigation of Hypericum perforatum L. Fresenius Environ Bull, 28(7): 5569-5576
Search in Google Scholar Back to article
Naguleswaran, A., Spicher, M., Vonlaufen, N., Ortega-Mora, L.M., Torgerson, P., Gottstein, B., HemPHill, A. (2006): In vitro metacestodicidal activities of genistein and other isoflavones against Echinococcus multilocularis and Echinococcus granulosus. Antimicrob Agents Chemother, 50(11): 3770–3778. DOI: <a href="https://doi.org/10.1128/AAC.00578-06" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1128/AAC.00578-06</a>
Open DOI Search in Google Scholar Back to article
Nascimento, G.G., Locatelli, J., Freitas, P.C., Silva, G.L. (2000): Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol, 31: 247-256. DOI: <a href="https://doi.org/10.21010/ajtcaM.v14i1.23" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.21010/ajtcaM.v14i1.23</a>
Open DOI Search in Google Scholar Back to article
Nicolao, M.C., Elissondo, M.C., Denegri, G.M., Goya, A.B., Cumino, A.C. (2014): In vitro and in vivo effects of tamoxifen against larval stage Echinococcus granulosus. Antimicrob Agents Chemother, 58(9): 5146–5154. DOI: <a href="https://doi.org/10.1128/AAC.02113-13" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1128/AAC.02113-13</a>
Open DOI Search in Google Scholar Back to article
Noal, C.B., Monteiro, D.U., Brum, T.F., Emmanouilidis, J., Zanette, R.A., Morel, A.F., Stefanon, E.B.C., Frosi, M., Larue, M.L. (2017): In vitro effects of Blepharocalyx salicifolius (H.B.K.) O. Berg on the viability of Echinococcus ortleppi protoscoleceS. Rev Inst Med Trop Sao Paulo, 59: e42. DOI: <a href="https://doi.org/10.1590/S1678-9946201759042" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1590/S1678-9946201759042</a>
Open DOI Search in Google Scholar Back to article
Pensel, P.E., Maggiore, M.A., Gende, L.B., Eguaras, M.J., Denegri, M.G., Elissondo, M.C. (2014): Efficacy of essential oils of Thymus vulgaris and Origanum vulgare on Echinococcus granulosus. Interdiscip Perspect Infect Dis, 2014: 693289. DOI: <a href="https://doi.org/10.1155/2014/693289" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1155/2014/693289</a>
Open DOI Search in Google Scholar Back to article
Rasooli, I., Rezaei, M.B., Allameh, A. (2006): Ultrastructural studies on antimicrobial efficacy of thyme essential oils on Listeria monocytogenes. Int J Infect Dis, 10(3): 236–241. DOI: <a href="https://doi.org/10.1016/j.ijid.2005.05.006" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1016/j.ijid.2005.05.006</a>
Open DOI Search in Google Scholar Back to article
Rouhani, S., Salehi, N., Kamalinejad, M., Zayeri, F. (2013): Efficacy of Berberis vulgaris aqueous extract on viability of Echinococcus granulosus protoscoliceS. J Invest Surg, 26(6): 347-351. DOI: <a href="https://doi.org/10.3109/08941939.2013.818746" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.3109/08941939.2013.818746</a>
Open DOI Search in Google Scholar Back to article
SalmeróN-Manzano, E., Garrido-Cardenas, J.A., Manzano-Agugliaro, F. (2020): Worldwide research trends on medicinal plantS. Int J Environ Res Public Health, 17(10): 3376. DOI: <a href="https://doi.org/10.3390/ijerph17103376" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.3390/ijerph17103376</a>
Open DOI Search in Google Scholar Back to article
Sarikurkcu, C., Locatelli, M., Tartaglia, A., Ferrone, V., Juszczak, A.M., Ozer, M.S., TePe, B., Tomczyk, M. (2020): Enzyme and biological activities of the water extracts from the plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum thatare used as folk remedies in Turkey. Molecules, 25(5): 1202. DOI: <a href="https://doi.org/10.3390/molecules25051202" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.3390/molecules25051202</a>
Open DOI Search in Google Scholar Back to article
Sherif, M.M., Elshikh, H.H., Abdel-Aziz, M.M., Elaasser, M.M., Yosri, M. (2023): In Vitro Antibacterial and phytochemical screening of Hypericum perforatum extract as potential antimicrobial agents against multi-drug-resistant (MDR) strains of clinical origin. BioMed Res Int, 2023: 6934398. DOI: <a href="https://doi.org/10.1155/2023/6934398" target="_blank" rel="noopener noreferrer" class="text-signal-blue hover:underline">10.1155/2023/6934398</a>
Open DOI Search in Google Scholar Back to article
Vakili, Z., Radfar, M.H., Bakhshaei, F., Sakhaee, E. (2019): In vitro effects of Artemisia sieberi on Echinococcus granulosus protoscolices. Exp Parasitol, 197: 65–67. DOI: 10.1016j.exppara.2018.10.011
Search in Google Scholar Back to article
World Health Organization: Indicator Metadata Registry Details. Retrieved April 01, 2024, from https://www.who.int/data/gho/indicator-metadata-registry/imr-details/158
Search in Google Scholar Back to article
Zibaei, M., Sarlak, A., Delfan, B., Ezatpour, B., AzArgoon, A. (2012): Scolicidal effects of Olea europaea and Satureja khuzestanica extracts on protoscolices of hydatid cysts. Korean J Parasitol, 50(1): 53. DOI: 10.3347kjp.2012.50.1.53
Search in Google Scholar Back to article