Fig. 1.
Effect of T. vulgaris extract (150 mg/ml) on protoscoleces. The same microscopic image was photographed at one-minute intervals to observe how the protoscoleces took up the 0.1% eosin after their death (A: min 1, B: min 2, C: min 3, D: min 4, E: min 5, and magnification: 40X).
Table 1.
In vitro protoscolicidal activity of different concentrations of herbal extracts at 5, 10, and 60 min.
| Experimental Groups | Samples | Concentration | Mortality rate (%) with following exp. time | ||
|---|---|---|---|---|---|
| 5 min | 10 min | 60 min | |||
| Treatment with herbal extract | H. perforatum (Leaves and flowers) | 50 mg/ml 100 mg/ml 150 mg/ml | 36.4 ± 1.74 37.8 ± 1.98 38.9 ± 2.35 | 49.1 ± 1.35 52.2 ± 2.7 54.4 ± 1.35 | 88.9 ± 3.85 91.6 ± 3.7 93.3 ± 3.29 |
| T. vulgaris (Leaves) | 50 mg/ml 100 mg/ml 150 mg/ml | 33.7 ± 1.56 60.9 ± 2.25 96.5 ± 2.58 | 40.2 ± 2.19 66.2 ± 3.64 98 ± 1.15 | 56.9 ± 1.95 99.3± 0.39 99.8 ± 0.2 | |
| P. racemose (Leaves) | 50 mg/ml 100 mg/ml 150 mg/ml | 44.6 ± 3.53 55.1 ± 3.95 60.2 ± 2.73 | 52.2 ± 2.92 76.9 ± 2.99 96.8 ± 3.88 | 95.3 ± 2.31 96.7 ± 1.76 98.2 ± 0.97 | |
| M. piperita (Leaves) | 50 mg/ml 100 mg/ml 150 mg/ml | 10.44 ± 0.97 16 ± 1.77 17.3 ± 1.92 | 11.6 ± 1.98 16.9 ± 1.35 19.3 ± 2.03 | 14.2 ± 2.73 18.7 ± 1.15 20 ± 1.02 | |
| Positive Control | NaCl | 20 % | 100 ± 0 | 100 ± 0 | 100 ± 0 |
| Negative Control | NaCl | 0.90 % | 2.2 ± 0.46 | 3.3 ± 0.53 | 4.5 ± 0.97 |