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Redescription of Laimydorus siddiqii Baqri & Jana, 1982 (Dorylaimida: Dorylaimidae) with its first molecular study Cover

Redescription of Laimydorus siddiqii Baqri & Jana, 1982 (Dorylaimida: Dorylaimidae) with its first molecular study

Open Access
|May 2025

Figures & Tables

Fig. 1.

Laimydorus siddiqii (Female) from Uttarakhand, India (light microscopy). A: Anterior region with double guiding ring (arrowed); B: Pharyngeal expansion; C: Entire female; D: Posterior region; E: Prerectum; F: Tail region; G: Genital system; H: Cardiac region; I: Anterior ovary J: Vagina (arrowed); K: Posterior ovary. Scale bars: (A,G,H,I,K), 10 μm; (B,D,E,F) 20 μm; (C)100 μm.
Laimydorus siddiqii (Female) from Uttarakhand, India (light microscopy). A: Anterior region with double guiding ring (arrowed); B: Pharyngeal expansion; C: Entire female; D: Posterior region; E: Prerectum; F: Tail region; G: Genital system; H: Cardiac region; I: Anterior ovary J: Vagina (arrowed); K: Posterior ovary. Scale bars: (A,G,H,I,K), 10 μm; (B,D,E,F) 20 μm; (C)100 μm.

Fig. 2.

Laimydorus siddiqii (Male) from Uttarakhand, India (light microscopy). A: Anterior body region; B: Pharyngeal expansion; C: Pharyngointestinal junction; D: Entire male; E: Anterior region with double guiding ring; F: Spicules; G: Posterior body region; Scale bars: (A,E,C), 10 μm; (D) 100 μm; (B,F,G), 20 μm.
Laimydorus siddiqii (Male) from Uttarakhand, India (light microscopy). A: Anterior body region; B: Pharyngeal expansion; C: Pharyngointestinal junction; D: Entire male; E: Anterior region with double guiding ring; F: Spicules; G: Posterior body region; Scale bars: (A,E,C), 10 μm; (D) 100 μm; (B,F,G), 20 μm.

Fig. 3.

Phylogenetic tree based on D2-D3 expansion segments of the 28S rRNA gene sequences of the nematodes isolated in this study and several related species. Phylogenetic relationships based on the nucleotide sequences of the D2-D3 expansion segments of the 28S rRNA gene were inferred by using the Maximum Likelihood method based on the General Time Reversible Model. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. NCBI accession numbers of the sequences used for the analyses are shown.
Phylogenetic tree based on D2-D3 expansion segments of the 28S rRNA gene sequences of the nematodes isolated in this study and several related species. Phylogenetic relationships based on the nucleotide sequences of the D2-D3 expansion segments of the 28S rRNA gene were inferred by using the Maximum Likelihood method based on the General Time Reversible Model. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. NCBI accession numbers of the sequences used for the analyses are shown.

Fig. 4.

Phylogenetic tree based on 18S rRNA sequences of the nematodes isolated in this study and several related species. Phylogenetic relationships based on 18S rRNA gene sequences were inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. NCBI accession numbers of the sequences used for the analyses are shown.
Phylogenetic tree based on 18S rRNA sequences of the nematodes isolated in this study and several related species. Phylogenetic relationships based on 18S rRNA gene sequences were inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. NCBI accession numbers of the sequences used for the analyses are shown.

Morphometry of Laimydorus siddiqii Baqri and Jana, 1982 from Uttarakhand, India_ All the measurements are in μm (except n, ratio and percentage) and are in the form of mean ± standard deviation (range)_

CharactersFemalesMales
N106
Total body length (L)2913 ± 162 (2601 – 3100)2710 ± 312 (2393 – 3108)
a (L/BD)38 ± 4.1 (31 – 42)35 ± 2.4 (33 – 39)
b (L/NL)5.1 ± 0.2 (4.8 – 5.2)4.6 ± 0.4 (4.2 – 5.2)
c (L/TL)12.1 ± 0.8 (11 – 14)110.14 ± 16.6 (94 – 141)
c' (T/ABD)7.9 ± 0.6 (6.5 – 8.9)0.8 ± 0.1 (0.7 – 1)
V % (AV/L) ×10048 ± 1.9 (45 – 52)
Lip region width15.7 ± 1.2 (14 – 18)15.2 ± 0.4 (15 – 16)
Lip region height5.7 ± 0.4 (5.1 – 6)5.9 ± 05 (5 – 5.6)
Amphid aperture width8.2 ± 1.3 (7 – 10)6.6 ± 0.57 (6 – 7)
G1((AUL + AOVL+ AOL)/L×100)22.4 ± 1.5 (20.6 – 25.6)
G2 ((PUL+ POVL+ POL/L) × 100)21.4 ± 1.9 (19.3 – 23.6)
Cuticle at anterior region1.85 ± 0.2 (1.5 – 2)1.9 ± 0.2 (1.4 – 2)
Cuticle at mid region2 ± 0.35 (1.5 – 2.5)1.2 ± 0.2 (1 – 1.5)
Cuticle at tail region2.52 ± 0.4 (2 – 3)2.9 ± 0.2 (2.5 – 3)
Odontostyle length31.5 ± 0.8 (30 – 33)31.9 ± 0.6 (31 – 33)
Odontostyle length-dorsal side21.5 ± 2.0 (19 – 26)21.7 ± 1 (20 – 23)
Odontostyle length-ventral side30.7 ± 1.4 (29 – 34)30.6 ± 0.8 (30 – 32)
Odontostyle width3.6 ± 0.6 (3.1 – 5.2)3.7 ± 0.5 (3 – 4)
Odontostyle aperture length10.5 ± 0.9 (9 – 12)10.2 ± 1.1 (9 – 12)
Odontophore length35.8 ± 2.2 (33 – 40)36.4 ± 0.8 (35 – 37)
Distance guiding ring-anterior end19.3 ± 0.7 (18 – 20)18.2 ± 0.7 (17 – 19)
Distance nerve ring-anterior end170.6 ± 9.6 (155 – 185)182.3 ± 27.5 (155 – 210)
Neck length (NL)583 ± 31.7 (532 – 652)581.6 ± 12.9 (563 – 598)
Pharyngeal expansion length283 ± 21.2 (244 – 306)282 ± 14.8 (255 – 299)
Pharyngeal expansion width38 ± 2.8 (35 – 44)46.5 ± 3.1 (44 – 51)
Body diameter at neck base77 ± 8.2 (65.2 – 86)67.6 ± 2.6 (63 – 70)
Cardia length24.9 ± 3.1 (19 – 30)24.1 ± 3.1 (21 – 30)
Cardia width19.4 ± 1.9 (18 – 24)16.3 ± 1.6 (14 – 18)
Body diameter at vulva78.1 ± 8.1 (65 – 86)
Anterior uterus length (AUL)272.3 ± 11.5 (250 – 290)
Anterior oviduct length (AOVL)190.4 ± 0.5 (190 – 191)
Anterior ovary length (AOL187.8 ± 3.5 (183 – 192)
Posterior uterus length (PUL)261.6 ± 18 (225 – 292)
Posterior oviduct length (POVL)173.5 ± 7.7 (160 – 182)
Posterior ovary length (POL188.7 ± 5.3 (180 – 198)
Vagina length21.5 ± 1.9 (20 – 26)
Distance vulva-anterior end (AV)1407 ± 93.2 (1241 – 1558)
Prerectum length188 ± 16.9 (167 – 210)333 ± 61.9 (290 – 404)
Rectum length42.5 ± 5.2 (34 – 48)65.2 ± 10.10 (53 – 80)
Anal body diameter (ABD)30.4 ± 1.3 (28 – 32)31.5 ± 2.6 (28 – 35)
Tail length (TL)213 ± 27.8 (183 – 268)26.5 ± 1.3 (25 – 28)
Vulva to anus1272 ± 99.2 (1124 – 1410)
Body diameter (BD)78.1 ± 8.1 (65 – 86)72.1 ± 5.1 (63 – 78)
Spicule length59.5 ± 2.8 (56 – 63)
Max. width of spicule10.0 ± 0.8 (9.0 – 11.0)
Distance of hump from anterior end of spicule18.5 ± 0.7 (18 – 19)
Spicule width at posterior tip5.2 ± 0.6 (4.7 – 6)
Ventromedian supplements27.3 ± 0.8 (26 – 28)
Distance between supplements1.6 ± 0.2 (1.5 – 2.0)
DOI: https://doi.org/10.2478/helm-2025-0006 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 62 - 71
Submitted on: Jul 7, 2024
Accepted on: Feb 28, 2025
Published on: May 24, 2025
Published by: Slovak Academy of Sciences, Mathematical Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 A. Ansari, A. H. Bhat, Z. Imran, A. K. Chaubey, D. Fouad, G. Pundir, published by Slovak Academy of Sciences, Mathematical Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.