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Ovicidal effect of ethanolic extracts of selected plants on eggs of liver flukes in vitro Cover

Ovicidal effect of ethanolic extracts of selected plants on eggs of liver flukes in vitro

By: M. Ahmed,  A. Elamin,  M. Baha Saeed and  M. Laing  
Open Access
|Dec 2024

Figures & Tables

Fig. 1.

The effects of ethanolic extracts of 29 plants on the mortality of eggs of liver flukes from cattle, observed for 15 days after treatment. Mortality was zero in the control treatment.
The effects of ethanolic extracts of 29 plants on the mortality of eggs of liver flukes from cattle, observed for 15 days after treatment. Mortality was zero in the control treatment.

Fig. 2:

In vitro efficacy of three concentrations of five ethanolic plant extracts against eggs of liver flukes from cattle. 5% versus 10%: y = 13.8x + 27.2 R2 = 0.9115; 5% versus 20%: y = 11.8x + 46.8 R2 = 0.8808 ▼– five plants at 20% concentrations;  – five plants at 10% concentrations; ● – five plants and bromelain at 5% concentrations
In vitro efficacy of three concentrations of five ethanolic plant extracts against eggs of liver flukes from cattle. 5% versus 10%: y = 13.8x + 27.2 R2 = 0.9115; 5% versus 20%: y = 11.8x + 46.8 R2 = 0.8808 ▼– five plants at 20% concentrations; – five plants at 10% concentrations; ● – five plants and bromelain at 5% concentrations

List of plant species and the organs that were evaluated for their anthelmintic activity_

NoFamily NameScientific NameCommon NameOrgans Used
1.FabaceaeAcacia karooSweet thornLeaves and stem
2.FabaceaeAcacia tortilisUmbrella thornLeaves and stem
3.AsteraceaeAchillea millefoliumYarrowLeaves
4.LiliaceaeAllium sativumGarlicBulbs
5.AsphodelaceaeAloe feroxBitter aloeStem
6.BromeliaceaeAnanas comosusPineappleLeaves
7.PapaveraceaeArgemone mexicanaMexican prickly poppyLeaves and stem
8.AsteraceaeArtemisia afraAfrican wormwoodLeaves and stem
9.AsteraceaeArtemisia vulgarisMugwortLeaves and stem
10.AristolochiaceaeAsarum canadenseWild gingerRhizomes
11.AsteraceaeCichorium intybus.ChicoryLeaves
12.AmaryllidaceaeCrinum mooreiInanda lilyLeaves and stem
13.CyatheaceaeCyathea dealbataTree fernLeaves and stem
14.PoaceaeCymbopogon nardusLemon grassLeaves
15.ApiaceaeFoeniculum vulgareFennelStem and fruit
16.LamiaceaeMentha piperitaPeppermintLeaves and stem
17.MoringaceaeMoringa oleiferaHorse radish treeLeaves
18.LamiaceaeOcimum gratissimumAfrican basilLeaves and fruit
19.PoaceaeOxytenanthera abyssinicaLowland bambooStem
20.LamiaceaePlectranthus sp1Supr flowerLeaves and bark
21.LamiaceaePlectranthus sp2Supr flowerLeaves and bark
22.LamiaceaePlectranthus sp3Supr flowerLeaves and bark
23.LamiaceaePlectranthus sp4Supr flowerLeaves and bark
24.MyrtaceaePsidium guajavaGuavaLeaves
25.RubiaceaePsychotria capensisBird berryLeaves
26.MyrtaceaeSyzygium aromaticumCloveFruit
27.MyrtaceaeSyzygium cordatumWater berryLeaves
28.CanellaceaeWarburgia ugandensisEast African pepperLeaves and bark
29.CanellaceaeWarburgia salutarisPepper barkLeaves and bark

In vitro efficacy of ethanolic extracts of five plants against eggs of liver flukes from cattle__

Plants5% Extract Mortality (%)10% Extract Mortality (%)20% Extract Mortality (%)
Moringa oleifera82.67 fg90 gf100 h
Ananas comosus80 fg89 gf100 h
Foeniculum vulgare63 de75 ef89 gf
Cymbopogon nardus33 a47 bc62 de
Artemisia afra30 a42 ab60 cd

ANOVA table for the activity of five plant extracts at three concentrations against eggs of liver flukes from cattle_

Source of variationd.f.s.s.m.s.F valueP value
Plants519631.73926.3467.51<0.001
Concentrations28405.814202.9172.26<0.001
Plants x concentrations101896.41189.643.260.005
Residual341977.5258.16
Total5331998.59

LSD13.51
Mean ± SE67.4 ± 6.23
CV%12.65

ANOVA table for the main effect of 29 ethanolic plant extracts against eggs of liver flukes from cattle_

Source of variationd.f.s.s.m.s.F valueP value
Plants2987998.223034.421320.08<0.001
Residual58549.8449.48
Total8988556.22

LSD5.032
Mean ± SE41.56 ± 2.514
CV%7.4
DOI: https://doi.org/10.2478/helm-2024-0036 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 300 - 307
Submitted on: Jun 3, 2024
|
Accepted on: Dec 30, 2024
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Published on: Dec 31, 2024
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2024 M. Ahmed, A. Elamin, M. Baha Saeed, M. Laing, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.