Morphomolecular identification of Polylabris lingaoensis Tingbao, Kritsky, and Jun, 2007 infecting the twobar Seabream (Acanthopagrus bifasciatus)

Alojayri, G.; Al-Quraishy, S.; Mohammed, O. B.; Al-Shaebi, E.; Abdel-Gaber, R.

doi:10.2478/helm-2024-0031

Figures & Tables

Photomicrographs of Polylabris lingaoensis. (A) Whole specimen. (B) Anterior part of prohaptor. (C) Detail of prohaptor. (D) Male copulatory organ and distal portion of seminal vesicle. (E) Germarium. (F) Testis. (G) Two clamp rows in the haptor. (H) Detail of haptoral clamps. (I) Structure of clamp. Note: ALS, antero-lateral sclerite; BS, buccal sucker; CL, clamps; G, Germarium; GC, gland cells; HA, haptor; MCO, male copulatory organ; Mo, mouth; MS, median sclerite; OE, oesophagus; PD, prostatic ducts; PH, pharynx; PLS, postero-lateral sclerite; TE, testes; UT, uterus; V, vitellaria; VA, vagina; VR, vitelline reservoir.

A consensus phylogenetic tree constructed with maximum likelihood (ML) and Neighbor-Joining (NJ) methods, inferred from the partial 28S rRNA. Numbers indicated at branch nodes are bootstrap values. Only bootstraps > 50% are shown.

A consensus phylogenetic tree constructed with maximum likelihood (ML) and Neighbor-Joining (NJ) methods, inferred from the partial CO1. Numbers indicated at branch nodes are bootstrap values. Only bootstraps > 50% are shown.

Monogenean species used in the phylogenetic analysis of COI gene of Polylabris lingaoensis_

Parasite species	Host species	Infection site	Locality	Reference
PP372692 Polylabris lingaoensis	Acanthopagrus bifasciatus	Gills	Saudi Arabia	Present study
PP372693 Polylabris lingaoensis	Acanthopagrus bifasciatus	Gills	Saudi Arabia	Present study
MZ273903 Polylabris sillaginae	Sillago flindersi	-	Australia	GenBank
MZ273902 Polylabris sillaginae	Sillago flindersi	-	Australia	GenBank
MZ273904 Polylabris sillaginae	Sillago flindersi	-	Australia	GenBank
MZ273905 Polylabris sillaginae	Sillago flindersi	-	Australia	GenBank
MZ273901 Polylabris sillaginae	Sillago flindersi	-	Australia	GenBank
MZ273900 Polylabris sillaginae	Sillago flindersi	-	Australia	GenBank
MZ273899 Polylabris sillaginae	Sillago flindersi	-	Australia	GenBank
MZ273898 Polylabris sillaginae	Sillago flindersi	-	Australia	GenBank
MZ273908 Polylabris australiensis	Engraulis australis	-	Australia	GenBank
MZ273907 Polylabris australiensis	Engraulis australis	-	Australia	GenBank
MZ273906 Polylabris australiensis	Engraulis australis	-	Australia	GenBank
JQ038230 Polylabroides guangdongensis	-	-	China	GenBank
NC016057 Polylabris halichoeres	-	-	-	GenBank
KX926447 Microcotyle sp.	Scorpaena notata	Gills	France	GenBank
OQ101616 Kannaphallus sp.	Gnathanodon speciosus	Gills	Australia	GenBank
LC664022 Kannaphallus leptosomus	Scyris indica	Gills	Malaysia	GenBank
MH090371 Cemocotyle carangis	Caranx latus	Gills	Brazil	GenBank
KP119318 Zeuxapta seriolae	Seriola lalandi	Gills	Chile	GenBank
KP119357 Zeuxapta seriolae	Seriola lalandi	Gills	Chile	GenBank
MT890370 Choricotyle brasiliensis	Orthopristis ruber	Gills	Brazil	GenBank
KJ794205 Choricotyle anisotremi	Anisotremus scapularis	Gills	Chile	GenBank
KJ794206 Choricotyle anisotremi	Anisotremus scapularis	Gills	Chile	GenBank
KJ794207 Choricotyle anisotremi	Anisotremus scapularis	Gills	Chile	GenBank
KJ794216 Parapedocotyle prolatili	Prolatilus jugularis	Gills	Chile	GenBank
MT783686 Choricotyle australiensis	Chrysophrys auratus	Gills	Australia	GenBank
MT783687 Choricotyle australiensis	Chrysophrys auratus	Gills	Australia	GenBank
MT783685 Choricotyle australiensis	Chrysophrys auratus	Gills	Australia	GenBank

Comparative metrical data for Polylabris lingaoensis and their congeneric species_

Comparable parameters		*Tingbao et al. (2007)*	*Bayoumy et al. (2015)*	Present study (2024)
Host		Ambassis gymnocephalus	Acanthopagrus bifasciatus	Acanthopagrus bifasciatus

Location		China	Egypt	Saudi Arabia

Body	Length	1356 (1130–1597)	1231 (1120–1426)	1398 (1275–1723)
Body	Width	239 (159–298)	256 (179–286)	280 (230–310)

Buccal sucker	Length	41 (34–48)	43 (34–48)	49 (42–61)
Buccal sucker	Width	43 (37–50)	46 (39–54)	54 (42–70)

Pharynx diameter		34 (27–42)	34 (27–42)	35 (30–52)

Oesophagus length		-	-	111 (102–120)

Number of genital spines		Unarmed	Unarmed	Unarmed

Male copulatory organ	Length	39 (34–45)	41 (37–48)	43 (39–50)
Male copulatory organ	Width	27 (22–33)	29 (25–36)	35 (30–45)

Germarium	Length	-	-	394 (387–411)
Germarium	Width	-	-	93 (81–102)

Vaginae diameter		-	-	49 (48–50)

Number of testes		5–7	6–8	6–8

Haptor	Length	583 (478–735)	623 (528–814)	1498 (1452–1511)
Haptor	Width	-	-	216 (207–232)

Number of clamp pairs		30–43	33–45	35–45

Anterior clamp	Length	-	-	58 (52–63)
Anterior clamp	Width	43 (39–48)	-	33 (30–34)

Posterior clamp	Length	-	-	22 (21–25)
Posterior clamp	Width	36 (33–40)	-	37 (36–38)

Monogenean species used in the phylogenetic analysis of 28S rRNA gene of Polylabris lingaoensis_

Parasite species	Host species	Infection site	Locality	Reference
PP375821 Polylabris lingaoensis	Acanthopagrus bifasciatus	Gills	Saudi Arabia	Present study
PP375822 Polylabris lingaoensis	Acanthopagrus bifasciatus	Gills	Saudi Arabia	Present study
PP375823 Polylabris lingaoensis	Acanthopagrus bifasciatus	Gills	Saudi Arabia	Present study
PP375824 Polylabris lingaoensis	Acanthopagrus bifasciatus	Gills	Saudi Arabia	Present study
MT680612 Polylabris cf. mamaevie	Siganus fuscescens	Gills	China	GenBank
MH700257 Polylabris sp.	-	-	China	GenBank
MH700591 Polylabris cf. mamaevi	-	-	China	GenBank
GU289509 Polylabris sillaginae	Sillaginodes punctatus	Gills	Australia	GenBank
OR613028 Polylabris japonicus	Acanthopagrus schlegeli	-	South Korea	GenBank
MH700258 Polylabroides sp.	-	-	China	GenBank
MH700259 Lutianicola sp.	-	-	China	GenBank
ON792415 Sparicotyle chrysophryii	Sparus aurata	Gills	Tunisia	GenBank
GU263832 Kahawaia truttae	Arripis trutta	Gills	Australia	GenBank
GU263831 Kahawaia truttae	Arripis trutta	Gills	Australia	GenBank
KT191025 Microcotylidae sp.	Dentex dentex	Gills	Greece	GenBank
MN814848 Microcotyle erythrini	Pagellus erythrinus	Gills	Spain	GenBank
MN814847 Microcotyle whittingtoni	Dentex dentex	Gills	Spain	GenBank
LC658939 Paraheterobothrium chilense	Hippoglossina macrops	-	Japan	GenBank
MT890125 Echinopelma brasiliensis	Pagrus pagrus	-	Brazil	GenBank
MT782270 Choricotyle australiensis	Chrysophrys auratus	Gills	Australia	GenBank
AF382046 Choricotyle australiensis	Choricotyle australiensis	-	Australia	GenBank

Morphomolecular identification of Polylabris lingaoensis Tingbao, Kritsky, and Jun, 2007 infecting the twobar Seabream (Acanthopagrus bifasciatus)

Figures & Tables

Fig. 1.

Fig. 2.

Fig. 3.

Monogenean species used in the phylogenetic analysis of COI gene of Polylabris lingaoensis_

Comparative metrical data for Polylabris lingaoensis and their congeneric species_

Monogenean species used in the phylogenetic analysis of 28S rRNA gene of Polylabris lingaoensis_

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