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Fig. 6.

Locations where the EPNs were collected in southern Việt Nam_ These nematodes were collected in 2022_
| EPN Species | Location | GPS coordinates |
|---|---|---|
|
| 11.7748°N, 109.2048°E, 9 m ASL |
|
| 11.7858°N, 109.1899 °E, 9 m ASL |
|
| 12.2684°N, 109.1406°E, 580 m ASL |
|
| 11.7748 °N, 109.2048 °E, 9 m ASL |
Median lethal times (LT50) for Heterorhabditis indica, Oscheius myriophila, Steinernema carpocapsae, S_ eapokense and S_ pakistanense against third instar BMSB nymphs_
| EPN | Slope | LT50 | Lower 95% limit | Upper 95% limit | χ2-test | df | Sig. |
|---|---|---|---|---|---|---|---|
| Heterorhabditis indica | 1.185 | 6.195 | 5.188 | 8.594 | 11.379 | 16 | 0.785 |
| Oscheius myriophilus | 1.367 | 5.45 | 4.711 | 6.727 | 1.733 | 16 | 1 |
| Steinernema carpocapsae | 0.62 | 3.787 | 2.204 | 5.38 | 1.678 | 16 | 1 |
| Steinernema eapokense | 0.69 | 6.645 | 4.966 | 18.019 | 1.228 | 16 | 1 |
| Steinernema pakistanense | 0.554 | 2.955 | 0.702 | 4.136 | 1.944 | 16 | 1 |
Median lethal time (LT50) of Heterorhabditis indica, Oscheius myriophila, Steinernema carpocapsae and S_ pakistanense against adult BMSB_
| EPN species | Slope | LT50 | Lower 95% limit | Upper 95% limit | χ2-test | df | Sig. |
|---|---|---|---|---|---|---|---|
| Heterorhabditis indica | 2.099 | 2.975 | 2.577 | 3.322 | 16.2 | 16 | 0.639 |
| Oscheius myriophilus | 0.795 | 5.606 | 4.418 | 9.349 | 0.904 | 16 | 1 |
| Steinernema carpocapsae | 2.106 | 1.618 | 0.969 | 1.994 | 5.73 | 16 | 0.991 |
| Steinernema pakistanense | 1.252 | 3.362 | 2.701 | 3.93 | 3.191 | 16 | 1 |
Mean numbers of nematodes recorded in cadavers of third instar BMSB nymphs following death of nymphs treated with 100 to 1000 infective juveniles (IJs) of Heterorhabditis indica, Oscheius myriophila, Steinernema carpocapsae, S_ eapokense and S_ pakistanense_
| EPN species | Nematode concentration (IJs) applied | |||||
|---|---|---|---|---|---|---|
| 1000 | 800 | 600 | 400 | 200 | 100 | |
| Heterorhabditis indica | 6.27aC | 4.07aBC | 3.4aBC | 2.8aAB | 1.73aAB | 0.87aA |
| Oscheius myriophilus | 10.47aC | 5.4aB | 2.33aAB | 1.93aA | 1.4aA | 0.8aA |
| Steinernema carpocapsae | 28.8bE | 20.07bDE | 12.33bCD | 9.2bBC | 5.47bB | 2.13bA |
| Steinernema eapokense | 13.13aC | 8.13aBC | 4.8aAB | 3.071aAB | 3.27abcAB | 2.53A |
| Steinernema pakistanense | 38.33bF | 31.2bEF | 16.53bCD | 11.07bBC | 6.27bAB | 2.47bA |
Median lethal concentrations (LC50) of Heterorhabditis indica, Oscheius myriophila, Steinernema carpocapsae, S_ eapokense and S_ pakistanense against third instar BMSB nymphs_ df = degrees of freedom_
| EPN species | Slope | LC50 | Lower 95% limit | Upper 95% limit | χ2-test | df | Sig. |
|---|---|---|---|---|---|---|---|
| Heterorhabditis indica | 1.015 | 248 | 113 | 380 | 9.638 | 16 | 0.885 |
| Oscheius myriophilus | 0.845 | 247 | 79 | 412 | 4.349 | 16 | 0.998 |
| Steinernema carpocapsae | 2.062 | 167 | 112 | 217 | 11.622 | 16 | 0.77 |
| Steinernema eapokense | 1.012 | 193 | 72 | 303 | 3.304 | 16 | 1 |
| Steinernema pakistanense | 1.484 | 118 | 53 | 178 | 4.878 | 16 | 0.996 |
Median lethal concentrations (LC50) of Heterorhabditis indica, Oscheius myriophila, Steinernema carpocapsae and S_ pakistanense against BMSB adults_
| EPN species | Slope | LC50 | Lower 95% limit | Upper 95% limit | χ2-test | df | Sig. |
|---|---|---|---|---|---|---|---|
| Heterorhabditis indica | 1.833 | 46 | 9 | 84 | 12.401 | 16 | 0.716 |
| Oscheius myriophilus | 1.447 | 122 | 53 | 183 | 5.865 | 16 | 0.989 |
| Steinernema carpocapsae | 1.383 | 31 | 0 | 63 | 1.791 | 16 | 1 |
| Steinernema pakistanense | 1.656 | 49 | 9 | 91 | 9.826 | 16 | 0.876 |
Mean numbers of nematodes recorded in cadavers of adults following death of adults treated with 100 to 1000 IJs of Heterorhabditis indica, Oscheius myriophila, Steinernema carpocapsae and S_ pakistanense
| EPN species | Nematode concentration (IJs) applied | |||||
|---|---|---|---|---|---|---|
| 1000 | 800 | 600 | 400 | 200 | 100 | |
| Heterorhabditis indica | 8.8aC | 6.2aBC | 9.6C | 6BC | 4.93aB | 0.93aA |
| Oscheius myriophilus | 16.27aD | 8.07aCD | 12.6CD | 7.33CD | 5.47abB | 1.27aA |
| Steinernema carpocapsae | 47.67bC | 38.47bC | 11.73B | 10.33B | 8.27bB | 3.07bA |
| Steinernema pakistanense | 11.67aD | 8.87aCD | 14.8CD | 7CD | 4.6abB | 1.67abA |