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First molecular observation on Mylonchulus hawaiiensis from South Africa Cover

First molecular observation on Mylonchulus hawaiiensis from South Africa

By: E. Shokoohi  
Open Access
|Apr 2024

Figures & Tables

Fig. 1.

Sampling location for M. hawaiiensis in Magoebaskloof mountain of Limpopo Province, South Africa. (A) South Africa map. (B) Magoebaskloof mountain. (C) sampling site.
Sampling location for M. hawaiiensis in Magoebaskloof mountain of Limpopo Province, South Africa. (A) South Africa map. (B) Magoebaskloof mountain. (C) sampling site.

Fig. 2.

Mylonchulus hawaiiensis (Cassidy, 1931) Goodey, 1951. (A) neck. (B-G) anterior end (arrow pointing to, F: reserve dorsal tooth; G: amphid). (H) egg (arrow pointing to vulva. (I) pharyngeal-intestinal junction. (J) entire body (arrow pointing to vulva). (K) female tail (arrow pointing to caudal glands).
Mylonchulus hawaiiensis (Cassidy, 1931) Goodey, 1951. (A) neck. (B-G) anterior end (arrow pointing to, F: reserve dorsal tooth; G: amphid). (H) egg (arrow pointing to vulva. (I) pharyngeal-intestinal junction. (J) entire body (arrow pointing to vulva). (K) female tail (arrow pointing to caudal glands).

Fig. 3.

Principal component analysis (PCA) plot for M. hawaiiensis based on the important morphological characters.
Principal component analysis (PCA) plot for M. hawaiiensis based on the important morphological characters.

Fig. 4.

Phylogenetic tree based on 18S rDNA, including M. hawaiiensis from South Africa.
Phylogenetic tree based on 18S rDNA, including M. hawaiiensis from South Africa.

Fig. 5.

Phylogenetic tree based on ITS rDNA, including M. hawaiiensis from South Africa.
Phylogenetic tree based on ITS rDNA, including M. hawaiiensis from South Africa.

Fig. 6.

Network analysis based on Median Neighbor Joining analysis of M. hawaiiensis.
Network analysis based on Median Neighbor Joining analysis of M. hawaiiensis.

Loading factor of the variables of the species of M_ hawaiiensis_

F1F2
L0.661−0.043
a0.555−0.394
b0.7830.388
c−0.548−0.346
c′−0.2910.808
V−0.162−0.463
Lip region width0.353−0.278
Buccal cavity length0.536−0.592
Buccal cavity width−0.2510.598
Dorsal tooth apex%0.2110.360
Tail length0.8420.480

Measurements of Mylonchulus hawaiiensis (Cassidy, 1931) Goodey, 1951 from South Africa [all measurements in µm and in the format: mean ± standard deviation (range)]_

Body length1183.5 ± 56.9 (1139 – 1259)
a36.2 ± 6.5 (30.7 – 45.6)
b3.3 ± 0.1 (3.3 – 3.4)
c36.6 ± 7.7 (29.3 – 47.5)
c′1.3 ± 0.4 (1.0 – 1.7)
V65.8 ± 0.8 (65 – 67)
G122.6 ± 5.1 (17 – 28)
G218.6 ± 2.8 (16 – 22)
Lip region diameter22.3 ± 1.3 (21 – 24)
Buccal cavity length30.0 ± 1.4 (28 – 31)
Buccal cavity diameter10.3 ± 1.3 (9 – 12)
Amphidial position to ant. end11.7 ± 5.1 (6 – 16)
Amphidial aperture diameter4.8 ± 0.5 (4 – 5)
Foramen ventral6.0 ± 0.8 (5 – 7)
Foramen dorsal5.5 ± 1.3 (4 – 7)
Dorsal tooth width3.9 ± 0.7 (3 – 5)
Dorsal tooth length8.0 ± 0.8 (7 – 9)
Dorsal tooth apex%68.6 ± 2.1 (67 – 71)
Nerve ring to ant. end92.3 ± 7.9 (82 – 99)
Excretory pore to ant. end105.3 ±12.5 (93 – 118)
Pharynx330.0 ± 12.3 (316 – 339)
Neck355.8 ± 12.8 (344 – 370)
Cardia length7.3 ± 2.2 (5 – 10)
Cardia diameter8.0 ± 1.4 (7 – 10)
Body diameter at neck34.5 ± 1.3 (33 – 36)
Body diameter at mid body33.5 ± 6.0 (25 – 39)
Body diameter at anus25.3 ± 1.5 (24 – 27)
Cuticle1.4 ± 0.3 (1.2 – 1.8)
Vagina9.0 ± 1.0 (8 – 10)
Anterior genital branch269.7 ± 55.6 (207 – 313)
Anterior ovary120.5 ± 26.2 (102 – 139)
Posterior genital branch223.0 ± 30.5 (202 – 258)
Posterior ovary106.0 ± 11.3 (98 – 114)
Rectum19.8 ± 2.1 (18 – 22)
Tail33.5 ± 7.9 (24 – 43)

Factor score for the species of M_ hawaiiensis from different locations_

ObservationSample size (n)ReferenceF1F2
Hawaii1Cassidy, 19311.821−1.743
South Africa16Coetzee, 19661.7640.214
Nigeria18Mulvey & Jansen, 1967−0.274−0.352
India5Jairajpuri, 1970−1.0130.632
El Salvador2Baqri & Jairajpuri, 1974−2.6870.262
India200Khan & Jairajpuri, 1979−2.1682.261
India28Patil & Khan, 19820.0061.089
India1Jairajpuri & Khan, 1982−1.0970.580
Argentina10Chaves, 19901.1100.365
Costa Rica1Zullini et al., 20020.402−1.212
Iran5Shokoohi et al., 20132.0051.819
Iran10Shokoohi & Moyo, 20221.9851.403
Indonesia4Pradana & Yoshiga, 2023−2.832−2.211
South Africa10present study0.977−3.106

The comparative measurements for M_ hawaiiensis populations_

ReferenceLocationLabcc′VLip region widthBuccal cavity lengthBuccal cavity widthDorsal tooth apex%Tail length
Cassidy 1931Hawaii100033.33.6251.36127.127137940
Coetzee 1966South Africa800 – 150019 – 303.1 – 4.121 – 351.853 – 6328.830.317.48245.5
Mulvey & Jansen, 1967Nigeria900 – 130022 – 323.1 – 4.026.3 – 43.51.554 – 6222.520 – 2311.0 – 13.07323 – 42
Jairajpuri, 1970India870 – 98022 – 263.2 – 3.627 – 311.8 – 1.958 – 6020 – 2522 – 2514 – 177930 – 40
Baqri & Jairajpuri, 1973El Salvador600 – 84019 – 203.0 – 3.125 – 2760 – 6320 – 2323 – 35
Khan & Jairajpuri, 1979India700 – 135017 – 302.9 – 4.025 – 5450 – 7017 – 3010.0 – 16.065 – 8524 – 45
Patil & Khan, 1982India780 – 130021 – 302.6 – 3.822 – 301.5 – 2.054 – 6120 – 2521 – 2611.0 – 17.057 – 7537 – 43
Jairajpuri & Khan, 1982India700 – 135017 – 302.9 – 4.025 – 541.951 – 5816 – 2917 – 3010.0 – 16.070 – 8524 – 45
Chaves 1990Argentina960 – 137023.7 – 32.03.2 – 3.920.0 – 33.41.5 – 1.855 – 6023 – 2522 – 2715.0 – 17.073 – 7734 – 49
Zullini et al., 2002Costa Rica1200223.5351.35925271434
Shokoohi et al., 2013Iran925 – 113719.3 – 28.83.3 – 4.219.2 – 27.01.4 – 2.050 – 6221 – 3024 – 3111.0 – 17.057 – 7538 – 49
Shokoohi and Moyo, 2022Iran1195243.723.51.755.32427.314.86443.3
Pradana & Yoshiga, 2023Indonesia622 – 130015.6 – 23.32.2 – 4.036.7 – 37.81.3 – 1.550 – 6123 – 3427 – 3014 – 157517 – 35
Present studySouth Africa1139 – 125930.7 – 45.63.3 – 3.429.3 – 47.51.0 – 1.765 – 6721 – 2428 – 319.0 – 12.067 – 7124 – 43
DOI: https://doi.org/10.2478/helm-2024-0010 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 99 - 108
Submitted on: Jun 4, 2023
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Accepted on: Nov 16, 2023
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Published on: Apr 23, 2024
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2024 E. Shokoohi, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.