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Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India) Cover

Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)

Helminthologia
Volume 60 (2023): Issue 3 (September 2023)
By: K. Satyaprakash,  W. A. Khan,  N. N. Zade,  S. P. Chaudhari,  S. V. Shinde,  N. V. Kurkure and  P. K. Shembalkar  
Open Access
|Dec 2023

Figures & Tables

Fig. 1.

(A–E) ELISA OD values using WCA (Fig. 1A), CFA (Fig. 1B), SA (Fig. 1C), ESA (Fig. 1D) and MBA (Fig. 1E) against sera samples from epileptic patients (n=26). The horizontal bar denotes cut-off.
(A–E) ELISA OD values using WCA (Fig. 1A), CFA (Fig. 1B), SA (Fig. 1C), ESA (Fig. 1D) and MBA (Fig. 1E) against sera samples from epileptic patients (n=26). The horizontal bar denotes cut-off.

Fig. 2.

(A–B) Number (Fig. 2A) and reactivity frequency (Fig. 2B) of immunodominant bands recognized by anti-cysticercus antibodies in the sera of patients (n=26) against respective antigens.
(A–B) Number (Fig. 2A) and reactivity frequency (Fig. 2B) of immunodominant bands recognized by anti-cysticercus antibodies in the sera of patients (n=26) against respective antigens.

Fig. 3.

Agarose gel electrophoresis showing amplification product (286 bp) by PCR targeted at large subunit rRNA gene of Taenia solium for detection of NCC in epileptic patients. The numbers (1–26) designate the patients under study. DNA extracted from metacestodes of T. solium is used as positive control and nuclease free water as negative control respectively.
Agarose gel electrophoresis showing amplification product (286 bp) by PCR targeted at large subunit rRNA gene of Taenia solium for detection of NCC in epileptic patients. The numbers (1–26) designate the patients under study. DNA extracted from metacestodes of T. solium is used as positive control and nuclease free water as negative control respectively.

Seropositivity by indirect IgG-ELISA according to location of lesions_

Location of lesionsNumber of patients (%)Anti-Cysticercus IgG-ELISA positivity
WCACFASAESAMBA
Left parietal lobe14 (53.84)4 (28.57)5 (35.71)7 (50.0)5 (35.74)3 (21.42)
Right parietal lobe2 (7.69)001 (50.0)1 (50.0)0
Left frontal lobe4 (15.38)1 (25.0)1 (25.0)01 (25.0)0
Right frontal lobe3 (11.53)00001 (33.0)
Occipital lobe1 (3.84)001 (100.0)00
Temporal lobe1 (3.84)00000
Basal ganglia1 (3.84)001 (100.0)1 (100.0)0
Total26 (100.0)5 (19.23)6 (23.07)10 (38.41)8 (30.76)4 (15.38)

Seropositivity by indirect IgG-ELISA and EITB assay against the antigens under study_

ScenarioNumber of patients showing seropositive results in ELISA and(or) EITB
WCACFASAESAMBA
Presence of both Anti-Cysticercus antibody in IgG-ELISA and Immunodominant band in EITB23764
Presence of Anti-Cysticercus antibody in IgG-ELISA, but absence of Immunodominant band in EITB33220
Absence of Anti-Cysticercus antibody in IgG-ELISA, but presence of Immunodominant band in EITB84115

Age and gender distribution of epileptic patients_

Age (years)Male (%)Female (%)Total (%)
< 153 (11.53)1 (3.84)4 (15.38)
15–298 (30.76)3 (11.53)11 (42.30)
30–447 (26.92)3 (11.53)10 (38.46)
≥451 (3.84)0 (0)1 (3.84)
Total19 (73.07)7 (26.93)26 (100.0)

Seropositivity result by indirect IgG-ELISA according to type of antigens and CT/MRI lesions*_

Type of lesionNumber of Patients (%)Number of patients seropositive against the antigens
WCA (%)CFA (%)SA (%)ESA (%)MBA (%)
Granular nodular4 (15.38)001 (25.0)00
Calcified granular13 (50.0)2 (15.38)3 (23.07)6 (46.15)5 (38.46)1 (7.69)
Ring enhancing6 (23.07)3 (50.0)2 (33.33)3 (50.0)3 (50.0)2 (33.33)
Calcified spots3 (11.53)01 (33.33)000
Total26 (100.0)5 (19.23)6 (23.07)10 (38.46)8 (30.76)4 (15.38)

Seropositivity by indirect IgG-ELISA according to number of lesions_

Number of lesionsNumber of patientsAnti-cysticercus IgG ELISA positivity
WCACFASAESAMBA
Single lesion19 (73.07)3 (15.78)2 (10.52)8 (42.14)4 (21.05)3 (15.78)
Multiple lesions7 (26.93)2 (28.57)4 (57.14)2 (28.57)4 (57.14)1 (14.28)
Total26 (100.0)5 (19.23)6 (23.07)10 (38.41)8 (30.76)4 (15.38)
DOI: https://doi.org/10.2478/helm-2023-0023 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Journal RSS Feed
Language: English
Page range: 208 - 220
Submitted on: Mar 10, 2021
|
Accepted on: Jul 7, 2023
|
Published on: Dec 26, 2023
Published by: Slovak Academy of Sciences, Institute of Parasitology
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open
Keywords:
Neurocysticercosis,
Antigens,
ELISA,
EITB,
PCR
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2023 K. Satyaprakash, W. A. Khan, N. N. Zade, S. P. Chaudhari, S. V. Shinde, N. V. Kurkure, P. K. Shembalkar, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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