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Discovery of a new genus and species of Allocreadiidae (Trematoda) in Mexico: Mesoamericatrema magnisacculus n. gen. n. sp Cover

Discovery of a new genus and species of Allocreadiidae (Trematoda) in Mexico: Mesoamericatrema magnisacculus n. gen. n. sp

Helminthologia
Volume 59 (2022): Issue 3 (September 2022)
By: B. Mendoza-Garfias,  J. G. García-Teh,  J. M. Caspeta-Mandujano,  V. M. Vidal-Martínez and  D. I. Hernández-Mena  
Open Access
|Dec 2022

Figures & Tables

Fig. 1

Mesoamericatrema magnisacculus n. sp.: a) Dorso-ventral view of the holotype (scale bar= 200 μm); a) Lateral view of a paratype (scale bar= 200 μm); c) and d) Ventral view of two different male genitalia, showing the cirrus sac containing the seminal vesicle, the pars prostatica, and the ejaculatory duct (scale bar= 100 μm). The seminal vesicle is embedded in a very thick sac.
Mesoamericatrema magnisacculus n. sp.: a) Dorso-ventral view of the holotype (scale bar= 200 μm); a) Lateral view of a paratype (scale bar= 200 μm); c) and d) Ventral view of two different male genitalia, showing the cirrus sac containing the seminal vesicle, the pars prostatica, and the ejaculatory duct (scale bar= 100 μm). The seminal vesicle is embedded in a very thick sac.

Fig. 2

Scanning electron micrographs of Mesoamericatrema magnisacculus n. sp.: A) ventral view of the entire body (scale bar= 100 μm); B) and C) ventral view of two different oral suckers showing the oral papillae (6 external anterior in blue arrows, 4 internal in green arrows and 5 external posterior in red arrows; scale bar= 25 μm); d) ventral view of the ventral sucker (scale bar= 25 μm); e) ventral view of terminal excretory pore at posterior end of hindbody (scale bar= 25 μm); f) close-up of an external oral papilla (scale bar= 5 μm); g) sperm leaving the genital pore (scale bar= 5 μm); h) egg released through the genital pore (scale bar= 25 μm).
Scanning electron micrographs of Mesoamericatrema magnisacculus n. sp.: A) ventral view of the entire body (scale bar= 100 μm); B) and C) ventral view of two different oral suckers showing the oral papillae (6 external anterior in blue arrows, 4 internal in green arrows and 5 external posterior in red arrows; scale bar= 25 μm); d) ventral view of the ventral sucker (scale bar= 25 μm); e) ventral view of terminal excretory pore at posterior end of hindbody (scale bar= 25 μm); f) close-up of an external oral papilla (scale bar= 5 μm); g) sperm leaving the genital pore (scale bar= 5 μm); h) egg released through the genital pore (scale bar= 25 μm).

Fig. 3

Photographs where the size of the cirrus bag is observed with respect to the size of the body. The length of the ejaculatory duct, the pars prostatica and the seminal vesicle, which is surrounded by a thick wall of the sac, are also observed; a), b) and c) paratypes stained with Mayer’s paracarmine; d) paratype stained with Gomori’s trichrome. Scale bar= 50 μm.
Photographs where the size of the cirrus bag is observed with respect to the size of the body. The length of the ejaculatory duct, the pars prostatica and the seminal vesicle, which is surrounded by a thick wall of the sac, are also observed; a), b) and c) paratypes stained with Mayer’s paracarmine; d) paratype stained with Gomori’s trichrome. Scale bar= 50 μm.

Fig. 4

Maximum Likelihood phylogenetic tree of the 28S gene for Allocreadiidae species, showing the position of the new genus that is highlighted in bold red font (–ln= 9888.861897). Numbers near internal nodes indicate bootstrap support and posterior probabilities values.
Maximum Likelihood phylogenetic tree of the 28S gene for Allocreadiidae species, showing the position of the new genus that is highlighted in bold red font (–ln= 9888.861897). Numbers near internal nodes indicate bootstrap support and posterior probabilities values.

Fig. 5

Phylogenetic tree of the species of Allocreadiidae with Neotropical distribution, indicating their definitive hosts and the families of fish to which they belong with Neotropical affinity.
Phylogenetic tree of the species of Allocreadiidae with Neotropical distribution, indicating their definitive hosts and the families of fish to which they belong with Neotropical affinity.

Trematode species included in phylogenetic analyses with the 28S rRNA gene_ The accession number of the Genbank sequences, the host and the locality where the sequenced specimens were collected are shown_

SpeciesAuthorGenBankHostLocalitySequence Reference
ALLOCREADIIDAELooss, 1902
Acrolichanus auriculatum(Wedl, 1858) Skvortsov, 1927MN524579Acipenser schrenckiiRussiaAtopkin et al., 2020
Allocreadium gotoi(Hasegawa & Ozaki, 1926) Shimazu, 1988LC215274Misgurnus anguillicaudatusJapan: Nagano, liyama, MidoriShimazu, 2017
Allocreadium isoporum(Looss, 1894) Looss, 1902GU462125Alburnus alburnusRussia: Lake Oster, KareliaPetkeviciüte et al., 2010
Allocreadium lobatumWallin, 1909EF032693Semotilus corporalisUSACurran et al., 2006
Allocreadium neotenicumPeters, 1957JX977132Hydroporus rufifronsUnited Kingdom: Lake District, CumbriaBray et al., 2012
Bunodera acerinaeRoitman & Sokolov, 1999GU462122Gymnocephalus cernuaRussia: River Tvertsa, upper Volga River basinPetkeviciüte et al., 2010
Bunodera eucaliae(Miller, 1936) Miller, 1940DQ029329Culaea inconstansCanada: Brokenhead River, ManitobaChoudhury and Leon-Regagnon, 2005
Bunodera inconstans(Lasee, Font & Sutherland, 1988) Brooks, 1992DQ029330Culaea inconstansCanada: Brokenhead River, ManitobaChoudhury and Leon-Regagnon, 2005
Bunodera luciopercae(Müller, 1776) Lühe, 1909GU462113Sphaerium rivicolaRussia: River Tvertsa, upper Volga River basinPetkeviciüte et al., 2010
Bunodera mediovitellataTsimbalyuk & Roitman, 1966DQ029332Gasterosteus aculeatusCanada: Little Campbell River, B. C.Choudhury and Leon-Regagnon, 2005
Bunodera sacculataVan Cleave & Mueller, 1932DQ029333Perea fluviatilisCanada: Lake Sasajewun, Algonquin Park, OntarioChoudhury and Leon-Regagnon, 2005
Bunodera vytautasiAtopkin, Sokolov, Shedko, Vainutis & Orlovskaya, 2018MG262545Pungitius pungitiusCanada: Magadan region, Chernoe LakeAtopkin et al., 2018
Crepidostomum affineTkach, Curran, Bell & Overstreet, 2013KF250358Hiodon tergisusUSA: Pearl River,MississippiTkach, et a/., 2013
Crepidostomum auritum(MacCallum, 1919)KF250357Aplodinotus grunniensUSA: Pearl River,MississippiTkach, et a/., 2013
Crepidostomum cooperiHopkins, 1932DQ029328Percopsis omiscomaycusCanada: Lake Winnipeg, ManitobaChoudhury and Leon-Regagnon, 2005
Crepidostomum cornutum(Osborn, 1903) Stafford, 1904EF032695Lepomis gulosusUSACurran et al., 2006
Crepidostomum farionis(Müller, 1780) Lühe, 1909FR821399Oncorhynchus masouRussiaAtopkin and Shedko, 2014
Crepidostomum illinoienseFaust, 1918KF356372Hiodon alosoidesUSA: Red Lake River,MinnesotaTkach, et a/., 2013
Crepidostomum metoecus(Braun, 1900) Braun, 1900FR82140Salvelinus leucomaensisRussiaAtopkin and Shedko, 2014
Crepidostomum nemachilusKrotov, 1959FR821408Salvelinus leucomaensisRussiaAtopkin and Shedko, 2014
Crepidostomum oschmariniZhokhov & Pugacheva, 1998MH159994Pisidium casertanumLithuania: River NedzingePetkeviciüte et al., 2018
Creptotrema astyanace(Scholz, Aguirre-Macedo & Choudhury, 2004) Franceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021HQ833707Astyanax aeneusCosta Rica: GuanacasteCurran etal., 2011
Creptotrema stenopteri(Mane-Garzon & Gascon, 1973) Franceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021MN822005Charax stenopteriArgentina: Buenos Aires, Punta LaraMontes et al., 2021
Creptotrema conconaeFranceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021OK044374Imparfinis miriniBrazil: Upper Parana River basinFranceschini et al., 2021
Creptotrema creptotremaTravassos, Artigas & Pereira, 1928OK044371Megaleporinus elongatusBrazil: Mogi-Guacu River, Upper Parana River basinFranceschini et al., 2021
Creptotrema funduliMueller, 1934JQ425256Fundulus notatusUSA: Mississippi, Biloxi River, Harrison CountyCurran et al., 2012
Creptotrema guacurarii(Montes, Barneche, Croci, Balcazar, Almiron, Martorelli & Perez-Ponce de Leon, 2021) Franceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021MN822004Characidium heirmostigmataArgentina: Misiones, Parque Nacional IguazuMontes et al., 2021
Creptotrema lobata(Hernändez-Mena, Lynggaard, Mendoza-Garfias & Perez- Ponce de Leon, 2016) Franceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021KX954170Brycon guatemalensisMexico: Tenosique, ChiapasHernändez-Mena etal., 2016
Creptotrema megacetabularisFranceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021OK044375Auchenipterus osteomystaxBrazil: Upper Parana River basinFranceschini et al., 2021
Creptotrema schubartiFranceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021OK044373Characidium schubartiBrazil: Upper Parana River basinFranceschini et al., 2021
Creptotrema tica(Hernandez Mena, Pinacho-Pinacho, Garcia-Varela, Mendoza- Garfias & Perez-Ponce de Leon, 2018) Franceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021MH997001Gymnotus maculosusCosta Rica: Creek at Pitaya, GuanacasteHernändez-Mena etal., 2019
Creptotrema totonacapanensis(Razo-Mendivil, Mendoza-Garfias, Perez- Ponce de Leon & Rubio-Godoy, 2014) Franceschini, Aguiar, Zago, de Oliveira Fadel Yamada, Bertholdi Ebert & da Silva, 2021KF631417Astyanax mexicanusMexico: Filipinas, VeracruzRazo-Mendivil et al., 2014b
Creptotrematina aguirrepequenoiJimenez-Guzman, 1973HQ833708Astyanax aeneusCosta Rica: GuanacasteCurran etal., 2011
Creptotrematina batalhensisDias & Abdallah in Alves Dias, Perez-Ponce de Leon, Almeida Camargo, Müller, Silva, Kozlowiski de Azevedo & Abdallah, 2020MT512642Astyanax lacustrisBrazil: Batalha RiverDias et al., 2020
Margotrema bravoaeLamothe-Argumedo, 1970KT833278Allotoca dugesiiMexico (Central region)Perez-Ponce de Leon et al., 2016
Margotrema resolanaePerez-Ponce de Leon, Martinez-Aquino & Mendoza-Garfias, 2013KT833271Xenotaenia resolanaeMexico (Central region)Perez-Ponce de Leon et al., 2016
Megalogonia ictaluriSurber, 1928EF032694Ictalurus punctatusUSA: Pearl River, MississippiCurran et al., 2006
Mesoamericatrema magnisacculusThis studyOP279638- OP279642Atherinella alvareziMexico: Arroyo San Jose, ChiapasThis study
Paracreptotrema blancoiChoudhury, Perez Ponce de Leon, Brooks & Daverdin, 2006KT833285Priapichthys annectensCosta Rica: Orosi RiverPerez-Ponce de Leon et al., 2016
Paracreptotrema rosenthaliBautista-Hernandez, Monks, Pulido-Flores & Miranda, 2015KT833287Xiphophorus malincheMexico: Malila River, HidalgoPerez-Ponce de Leon et al., 2016
Paracreptotrematoides heterandriae(Salgado-Maldonado, Caspeta-Mandujano & Vazquez, 2012) Perez Ponce de Leon, Pinacho-Pinacho, Mendoza-Garfias, Choudhury & Garcia-Varela, 2016KF697693Heterandria bimaculataMexico: Agua Bendita, Xico, VeracruzRazo-Mendivil et al., 2014a
Pseudoparacreptotrema axtlaensisMendoza-Garfias & Choudhury, 2020MT180832Agonostomus monticolaMexico: Axtla de Terrazas, San Luis PotosiPerez-Ponce de Leon et al., 2020
Pseudoparacreptotrema falciformisHernändez-Mena & Pinacho-Pinacho, 2020MT180829Agonostomus monticolaMexico: Matias Romero, OaxacaPerez-Ponce de Leon et al., 2020
Pseudoparacreptotrema macroacetabulataPerez Ponce de Leon, Pinacho-Pinacho, Mendoza-Garfias, Choudhury & Garcia-Varela, 2016KT833322Profundulus kreiseriGuatemala: Puente SansarePerez-Ponce de Leon et al., 2016
Pseudoparacreptotrema pacificumSereno-Uribe & Garcia-Varela, 2020MT180810Agonostomus monticolaCosta Rica: Rio LajasPerez-Ponce de Leon et al., 2020
Pseudoparacreptotrema profundulusi(Salgado-Maldonado, Caspeta-Mandujano & Martinez- Ramirez, 2011) Perez Ponce de Leon, Pinacho-Pinacho, Mendoza-Garfias, Choudhury & Garcia-Varela, 2016KT833290Profundulus sp.Mexico: Templo River, San Juan del Rio, OaxacaPerez-Ponce de Leon et al., 2016
Wallinia anindoiHernändez Mena, Pinacho-Pinacho, Garcia-Varela, Mendoza- Garfias & Perez-Ponce de Leon, 2018MH997003Astyanax aeneusMexico: San Juan del Rio, OaxacaHernändez Mena et al., 2018
Wallinia brasiliensisDias, Müller, Almeida, Silva, Azevedo, Perez-Ponce de Leon & Abdallah, 2018MH520995Astyanax fasciatusBrazil: Upper Paranä River BasinDias et al., 2018
Wallinia caririensisSilva, Dias, Silva & Yamada, 2020MW024866Astyanax bimaculatusBrazil: Batateiras Riverda Silva et al., 2021
Wallinia chavarriaeChoudhury, Daverdin & Brooks, 2002HC833703Astyanax aeneusCosta Rica: Animas River, GuanacasteCurran et al., 2011
Wallinia mexicanaPerez-Ponce de Leon, Razo-Mendivil, Mendoza-Garfia, Rubio-Godoy & Choudhury, 2015KJ535504Astyanax mexicanusMexico: Covadonga River, DurangoPerez-Ponce de Leon et al., 2016
CALLODISTOMIDAEOdhner, 1910
Prosthenhystera caballeroiJimenez-Guzmän, 1973KM871185Astyanax aeneusCosta Rica: Tampisquto River, GuanacasteTkach and Curran, 2015
Prosthenhystera obesa(Diesing, 1850) Travassos, 1922AY222206Hoplias sp.Peru: Itaya River, IquitosOlson et al., 2003
Prosthenhystera oonasticaTkach & Curran, 2015KM871181Pylodictis olivarisUSA: Pearl River, MississippiTkach and Curran, 2015

Genetic distances between Mesoamericatrema magnisacculus n_ gen_ n_ sp_ and the Allocreadiidae species of the Neotropical clade in the 28S and ITS ribosomal genes (na= sequences not available in Genbank)_

SpeciesMesoamericatrema magnisacculus
28SITS
Creptotrema schubarti5.63na
Creptotrema guacurarii5.37na
Creptotrema creptotrema5.53na
Creptotrema tica3.75na
Creptotrema cf. stenopteri5.01na
Creptotrema megacetabularis4.71na
Creptotrema totonacapanensis3.834.94
Creptotrema conconae6.42na
Creptotrema lobata3.754.63
Creptotrema astyanace3.794.40
Wallinia brasiliensis5.20na
Wallinia anindoi5.43na
Wallinia caririensis6.24na
Wallinia mexicana5.76na
Wallinia chavarriae3.945.12
Creptotrematina batalhensis5.464.81
Creptotrematina aguirrepequenoi4.595.65
Paracreptotrema blancoi3.917.29
Paracreptotrema rosenthali5.698.27
Paracreptotrematoides heterandriae4.624.34
Pseudoparacreptotrema axtlaense5.03na
Pseudoparacreptotrema falciforme4.86na
Pseudoparacreptotrema macroacetabulatum3.937.56
Pseudoparacreptotrema pacificum5.61na
Pseudoparacreptotrema profundulusi5.297.56
DOI: https://doi.org/10.2478/helm-2022-0029 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 284 - 300
Submitted on: Aug 3, 2022
Accepted on: Aug 30, 2022
Published on: Dec 17, 2022
Published by: Slovak Academy of Sciences, Mathematical Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
phylogeny,
Chiapas,
Allocreadiidae,
new genus
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2022 B. Mendoza-Garfias, J. G. García-Teh, J. M. Caspeta-Mandujano, V. M. Vidal-Martínez, D. I. Hernández-Mena, published by Slovak Academy of Sciences, Mathematical Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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