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Occurrence and seasonal changes in the population of root-knot nematodes on honeybush (Cyclopia sp.) Cover

Occurrence and seasonal changes in the population of root-knot nematodes on honeybush (Cyclopia sp.)

Helminthologia
Volume 58 (2021): Issue 2 (June 2021)
By: F. Y. Daramola,  R. Malgas and  A. P. Malan  
Open Access
|Jun 2021

Figures & Tables

Fig. 1

Google Earth image showing the location of the sampling sites in the Western Cape province of South Africa.
Google Earth image showing the location of the sampling sites in the Western Cape province of South Africa.

Fig. 2

Mean population density of root-knot nematode (RKN) and other plant-parasitic nematode (PPN) associated with honeybush cultivation on six sites at Genadendal, Western Cape province of South Africa (2016-2017). Error bars represent standard errors (±SE).
Mean population density of root-knot nematode (RKN) and other plant-parasitic nematode (PPN) associated with honeybush cultivation on six sites at Genadendal, Western Cape province of South Africa (2016-2017). Error bars represent standard errors (±SE).

Fig. 3

Mean population density of root-knot nematode (RKN) associated with honeybush cultivation at six sampling sites in Genadendal, Western Cape province of South Africa (2016-2017). Error bars represent standard errors (±SE).
Mean population density of root-knot nematode (RKN) associated with honeybush cultivation at six sampling sites in Genadendal, Western Cape province of South Africa (2016-2017). Error bars represent standard errors (±SE).

Fig. 4

Seasonal fluctuation and changes in the nematode population during the sampling period of 2016-2017 at the six experimental sites in Genadendal, Western Cape province of South Africa.
Seasonal fluctuation and changes in the nematode population during the sampling period of 2016-2017 at the six experimental sites in Genadendal, Western Cape province of South Africa.

Fig. 5

Symptoms of RKN damage on honeybush roots.
Symptoms of RKN damage on honeybush roots.

Fig. 6

A-B: Healthy flowering honeybush plants. C-D: Nematode damage on honeybush field, showing loss of plant.
A-B: Healthy flowering honeybush plants. C-D: Nematode damage on honeybush field, showing loss of plant.

Fig. 7

Gel pictures obtained from the amplification of DNA products of single J2s of RKN from honeybush plots. a. Meloidogyne javanica (amplified with FJav/Rjav primers) and b. M. hapla (with JMV1/JMV2 & JMV primers).
Gel pictures obtained from the amplification of DNA products of single J2s of RKN from honeybush plots. a. Meloidogyne javanica (amplified with FJav/Rjav primers) and b. M. hapla (with JMV1/JMV2 & JMV primers).

Temperature and rainfall distribution around Genadendal, Western Cape province (2016 – 2017)_

2016
2017
MonthTemperature °C (max)Temperature °C (min)Rainfall (mm)Temperature °C (max)Temperature 0C (min)Rainfall (mm)
January32.3719.340.2629.8916.291.4
February30.5916.820.3430.7317.480.14
March26.9516.061.1130.0915.070.09
April26.0813.630.8928.7914.340.92
May24.3111.220.3425.1111.790.33
June20.038.482.0520.447.510.82
July18.688.22.7218.967.090.4
August21.558.831.4717.287.551.65
September21.069.340.7720.568.660.58
October25.7411.230.2522.19.370.79
November28.0413.990.1824.2712.51.27
December31.0516.070.1926.9214.980.27

Primer codes used for identification of Meloidogyne species, sequences and sources_

SpeciesPrimerSequence 5'-3'Source
M. arenariaFarTCGGCGATAGAGGTAAATGACM. arenaria-specific SCAR
RarTCGGCGATAGACACTACAAACTZijlstra et al. (2000)
M. javanicaFjavGGTGCGCGATTGAACTGAGCM. javanica-specific SCAR
RjavCAGGCCCTTCAGTGGAACTATACZijlstra et al. (2000)
M. incognitaMI-FGTGAGGATTCAGCTCCCCAGM. incognita-specific SCAR
MI-RACGAGGAACATACTTCTCCGTCCMeng et al. (2004)
M. haplaJMV1GGATGGCGTGCTTTCAACM. hapla-, M. chitwoodi-and
JMV2TTTCCCCTTATGATGTTTACCCM. fallax-specific IGS-SCAR
JMVhaplaAAAAATCCCCTCGAAAAATCCACCWishart et al. (2002)
DOI: https://doi.org/10.2478/helm-2021-0018 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
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Language: English
Page range: 202 - 212
Submitted on: Jul 7, 2020
|
Accepted on: Feb 24, 2021
|
Published on: Jun 8, 2021
Published by: Slovak Academy of Sciences, Institute of Parasitology
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open
Keywords:
population density,
root-knot nematodes,
seasonal fluctuation
Related subjects:
Life sciences,
Zoology,
Ecology,
Life sciences, other,
Medicine,
Clinical medicine,
Microbiology, virology and infection epidemiology

© 2021 F. Y. Daramola, R. Malgas, A. P. Malan, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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