Table 1
Clustering behavior of protoscolices and visibility of components at different times after treated with lactophenol-based solutions and Proteinase-K lytic solution.
| Time of exposure | Lactophenol blue or lactophenol solution | Proteinase-K lytic solution |
|---|---|---|
| Time 30 minutes | Protoscolices aggregated/ Components and rostellar hooks not visible (Fig. 2a-b) | Protoscolices separated/ clearer vision of components (Fig. 2c-d) |
| Time 60 minutes | Protoscolices aggregated/ Components and rostellar hooks not visible (Fig. 3a-b) | Protoscolices separated/ clearer vision of components (Fig. 3c-d) |
| Time 90 minutes | Protoscolices aggregated/ clearer vision of components (Fig. 4a-b) | Protoscolices separated/ vision of components same as time 60 (Fig. 4c-d) |
| Time 120 minutes | Protoscolices partially separated/ components visible but with no lysis of membrane (Fig. 5a-b) | Protoscolices well separated/ starting of membrane lysis (Fig. 5c-d) |
| Time 150 minutes | Protoscolices partially separated/ components visible but with no lysis of membrane (Fig. 6a-b) | Protoscolices well separated/ increasing of membrane lysis (Fig. 6c-d) |
| Time 240 minutes | Protoscolices partially separated/ components visible (better with lactophenol) but with no lysis of membrane (Fig. 7a-b) | Protoscolices well separated/ membrane lysis semi-completed (Fig. 7c-d) Rostellar hooks free and visible (Fig. 8) |
Fig.1
Microscopic photography of protoscolices at different magnification (a, b) before use of lytic solutions (zero time), showing aggregation of protoscolices within the preservation solution (ethanol 70%).
Fig. 2
Microscopic photography of protoscolices after 30 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).
Fig. 3
Microscopic photography of protoscolices after 60 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).
Fig. 4
Microscopic photography of protoscolices after 90 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).
Fig. 5
Microscopic photography of protoscolices after 120 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d); arrows indicated the sites of lysis in cellular membrane.
Fig. 6
Microscopic photography of protoscolices after 150 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d).
Fig. 7
Microscopic photography of protoscolices after 240 min from zero time of addition of lactophenol blue (a), lactophenol solution (b), proteinase-K lytic solution (c, d), arrows indicated the sites of lysis in cellular membrane.
Fig. 8
Microscopic photography of hooks after lysis the membrane of protoscolices after 240 min from zero time of addition of proteinase-K lytic solution to the protoscolices.