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Clustering behavior of protoscolices and visibility of components at different times after treated with lactophenol-based solutions and Proteinase-K lytic solution_
| Time of exposure | Lactophenol blue or lactophenol solution | Proteinase-K lytic solution |
|---|---|---|
| Time 30 minutes | Protoscolices aggregated/ Components and rostellar hooks not visible (Fig. 2a-b) | Protoscolices separated/ clearer vision of components (Fig. 2c-d) |
| Time 60 minutes | Protoscolices aggregated/ Components and rostellar hooks not visible (Fig. 3a-b) | Protoscolices separated/ clearer vision of components (Fig. 3c-d) |
| Time 90 minutes | Protoscolices aggregated/ clearer vision of components (Fig. 4a-b) | Protoscolices separated/ vision of components same as time 60 (Fig. 4c-d) |
| Time 120 minutes | Protoscolices partially separated/ components visible but with no lysis of membrane (Fig. 5a-b) | Protoscolices well separated/ starting of membrane lysis (Fig. 5c-d) |
| Time 150 minutes | Protoscolices partially separated/ components visible but with no lysis of membrane (Fig. 6a-b) | Protoscolices well separated/ increasing of membrane lysis (Fig. 6c-d) |
| Time 240 minutes | Protoscolices partially separated/ components visible (better with lactophenol) but with no lysis of membrane (Fig. 7a-b) | Protoscolices well separated/ membrane lysis semi-completed (Fig. 7c-d) Rostellar hooks free and visible (Fig. 8) |