Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices

Hammad, S. J.; Cavallero, S.; Al-Nasiri, F. S.; Amelio, S. D᾿

doi:10.2478/helm-2020-0010

Abstract

Echinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar hooks of E. granulosus are useful for diagnosis and rostellar hook morphometric features may be useful to discriminate E. granulosus and related species. The present study was aimed to determine a more suitable lytic solution and to obtain a clearest vision for performing morphometric studies on the rostellar hooks of E. granulosus protoscolices. Five fertile hydatid cyst samples were collected from sheep in Kirkuk slaughterhouse, Iraq, during June of 2015. According to the results of the present study, proteinase-K lytic solution is the best approach in morphometric analysis to get a clear vision of rostellar hooks and a safer usage in comparison with solutions containing lactophenol (lactophenol, lactophenol blue).

References

Almeida, F.B., Rodrigues-Silva, R., Neves, R.H., Gonçalves, M.M.L., Romani, E.L.S., Machado-Silva, J.R. (2009): Morphological and morphometric studies on protoscolices rostellar hooks of Echinococcus granulosus from Peru visualized by several microscopic techniques. Neotrop. Helminthol., 3 (2): 65 – 72
Search in Google Scholar Back to article
Almeida, F.B., Rodrigues-Silva, R., Neves, R.H., Romani, E.L.S., Machado-Silva, J.R. (2007): Intraspecific variation of Echinococcus granulosus in livestock from Peru. Vet. Parasitol., 143: 50 – 58. DOI: 10.1016/j.vetpar.2006.07.028
Open DOI Search in Google Scholar Back to article
Al-Nasiri, F.S., Balbuena, J.A. (2018): Solostamenides iraqensis n. sp. (Monogenoidea, Microcotylidae ) parasitizing the freshwater mullet Liza abu (Pisces, Mugilidae) Iraq. Vie et milieu, 68 (4): 245 – 251
Search in Google Scholar Back to article
Arbabi, M., Pirestani, M., Delavari, M., Hooshyar, H., Abdoli, A., Sarvi, S. (2017): Molecular and morphological characterizations of Echinococcus granulosus from human and animal isolates on Kashan, Markazi Province, Iran. Iran J. Parasitol., 12 (2): 177 – 187
Search in Google Scholar Back to article
Blasco-Costa, I., Míguez-Lozano, R., Sarabeev, V., Balbuena, J.A. (2012): Molecular phylogeny of species of Ligophorus (Monogenea: Dactylogyridae) and their affinities within the Dactylogyridae. Parasitol. Int., 61: 619 – 627. DOI:10.1016/j.parint.2012.06.004
Open DOI Search in Google Scholar Back to article
Calderini, P., Gabrielli, S., Cancrini, G. (2012): Is the goat a new host for the G3 Indian buffalo strain of Echinococcus granulosus? Sci. World J., 2012: 1 – 5. DOI: 10.1100/2012/286357
Open DOI Search in Google Scholar Back to article
Constantine, C.C., Thompson, R.C.A., Jenkins, D.J., Hobs, R.P., Lymbery, A.J. (1993): Morphological characterization of adult Echinococcus granulosus as a means of determining transmission patterns. J. Parasitol., 79 (1): 57 – 61
Search in Google Scholar Back to article
Ebeling, W., Henrich, N., Klockow, M., Metz, H., Orth, H.d., Lang, H. (1974): Proteinase K from Tritirachium album Limber. Eur. J. Biochem., 47: 91 – 97
Search in Google Scholar Back to article
Eckert, J., Deplazes, P. (2004) Biological, epidemiological, and clinical aspects of Echinococcosis, a zoonosis of increasing concern. Clin. Microbiol. Rev., 17: 107 – 135. DOI:10.1128/CMR.17.1.107-135.2004
Open DOI Search in Google Scholar Back to article
Fadakar, B., Tabatabaei, N., Borji, H., Naghibi, A. (2015): Genotyping of Echinococcus granulosus from goats and sheep indicating G7 genotype in goats in the Northeast of Iran. Vet. Parasitol., 214 (1 – 2): 204 – 207. DOI:10.1016/j.vetpar.2015.09.029
Open DOI Search in Google Scholar Back to article
Harandi, M.F., Hajialilo, E., Shokouihi, M. (2012): Larval hook length measurement for differentiating G1 and G6 genotypes of Echinococcus granulosus sensu lato. Turkiye Parazitol. Derg., 36: 215 – 218. DOI:10.5152/tpd.2012.52
Open DOI Search in Google Scholar Back to article
Harvey, D. (2000): Nematode Analytical Chemistry. 1^st Edition, McGraw-Hill Com. NY., USA, 798 pp.
Search in Google Scholar Back to article
Heranández-Orts, J.S., Ahuir-Baraja, A.E., Raga, J.A., Montero, F.E. (2010): A new species of Empruthotrema (Monogenean: Monocotylidae) from Pteromylaeus bovinus (Myliobatidae) from the Western Mediterranean. J. Parasitol., 96 (6): 1081 – 1085
Search in Google Scholar Back to article
Hobs, R.P., Lymbery, A.J., Thomson, R.C.A. (1990): Rostellar hook morphology of Echinococcus granulosus (Batsch, 1786) from natural and experimental Australian hosts, and its implications for strain recognition. Parasitology, 101: 273 – 281
Search in Google Scholar Back to article
Humason, G.L. (1979) : Animal Tissue Techniques. 4^th Edition, W.H. Freeman and Company, San Francisco, 468 pp.
Search in Google Scholar Back to article
Jada, S., Raja, S., Jayakumar, K., Sahu, P. (2016): Incidence of intestinal parasitic infestation and anemia among school children in Ammapettai. Inter. J. Rec. Sci. Res., 7 (11): 14312 – 14316.
Search in Google Scholar Back to article
Karimi, A., Dianatpour, R. (2008): Genotyping and phenotyping characterization of Echinococcus granulosus of Iran. Biotechnology, 7 (4): 757 – 762
Search in Google Scholar Back to article
Kumaratilake, L.M., Thompson R.C.A. (1984): Morphological characterisation of Australian strains of Echinococcus granulosus. Int. J. Parasitol., 14 (4): 467 – 477
Search in Google Scholar Back to article
McManus, D.P., Zhang, W., Li, J., Bartley, P.B. (2003): Echinococcosis. Lancet, 362: 1295 – 1304
Search in Google Scholar Back to article
Moro, P.L., Schantz, P.M. (2009): Echinococcosis: a review. Int. J. Infect. Dis., 13 (2): 125 – 133. DOI:10.1016/j.ijid.2008.03.037
Open DOI Search in Google Scholar Back to article
Mowlavi, G., Salehi, M., Eshraghian, M., Rokni, M.B., Fasihi-Harandi, M., Mohajeran, E., Salahi-Moghaddam, A. (2012): Morphometric differentiation between camel and sheep strains of Echinococcus granulosus using computer image analysis system (CIAS). Asia. Pac. J. Trop. Med., pp. 58 – 21
Search in Google Scholar Back to article
Mustafa , I., Shabaz, M., Asif, S., Khan, M.R., Saeed, U., Sadiq, F., Mehmood, T., Ahmed, H., Simsek, S. (2015): Availability, cyst characteristics and hook morphology of Echinococcus granulosus Isolates from livestock (cattle, sheep and goats) in central Punjab, Pakistan. Kafkas Univ. Vet. Fak. Derg., 21 (6): 849 – 854. DOI:10.9775/kvfd.2015.13755
Open DOI Search in Google Scholar Back to article
Parija, S.C., Prabhakar, P.K. (1995): Evaluation of lacto-phenol cotton blue for wet mount preparation of feces. J. Clin. Microbiol., 33 (4): 1019 – 1021
Search in Google Scholar Back to article
Perez-Serano, J., Denegri, G., Casado, N., Bodega, G., Rodriguez-Cabeiro, F. (1995): Anti-tubulin immunohistochemistry study of Echinococcus granulosus protoscolices incubated with albendazole and albendazole sulphoxide in vitro. Parasitol. Res., 81: 438 – 440
Search in Google Scholar Back to article
Rodríguez-González, A., Míguez-Lozano, R., Llopis-Beleguer, C., Balbuena, J.A. (2015): A new species of Ligophorus (Monogenea: Dactylogyridae) from the gills of the flathead mullet Mugil cephalus (Teleostei: Mugilidae) from Mexico. Acta Parasitol., 60 (4): 767 – 776
Search in Google Scholar Back to article
Soriano, S.V., Pierangeli, N.B., Pianciola, L.A., Mazzeo, M., Lazzarini, L.E., Debiaggi, M.F., Bergagna, H.F.J., Basualdo, J.A. (2013): The optimum cut-off value to differentiate Echinococcus granulosus sensu stricto from other species of E. granulosus sensu lato using larval rostellar hook morphometry. J. Helminthol., 89: 1 – 8. DOI: 10.1017/S0022149X13000473
Open DOI Search in Google Scholar Back to article
Thompson, R.C.A., McManus, D.P. (2001): Aetiology: Parasites and Life-Cycles. In: Eckert, J., Gemmell, M.A., Meslin, F.X., Pawlowski, Z.S. (Eds.). WHO/ OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. OIE, Paris, pp. 1 – 19
Search in Google Scholar Back to article
Yildiz, K., Gurcan, I.S. (2009): The detection of Echinococcus granulosus strains using larval rostellar hook morphometry. Türkiye Parazitol. Derg., 33 (2): 199 – 202
Search in Google Scholar Back to article

Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices

Abstract

Paradigm

My account