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Validation of Gentamicin and Streptomycin Stability for On-Orbit Microbial Monitoring Cover

Validation of Gentamicin and Streptomycin Stability for On-Orbit Microbial Monitoring

Open Access
|Jul 2026

Figures & Tables

Figure 1.

Mechanisms of resistance for antibiotics included in literature review. Antibiotics are numbered based on order in table.1. Adapted from Arias & Murray 2012.

Figure 2.

Average colony-forming unit (CFU) counts for contact slides containing no antibiotic, low-concentration and high-concentration gentamicin, and low-concentration and high-concentration streptomycin over 6.5 months. Colored bars indicate standard deviation. Asterisks denote level of statistical significancy from single-factor ANOVA tests performed on CFU counts for the contact slides containing no antibiotic and low-concentration gentamicin and streptomycin (no significance: ns; p < 0.05: *; p < 0.01: **; p < 0.001: ***; p < 0.0001: ****).

Figure 3.

Average colony diameter on contact slides containing no antibiotic, low-concentration gentamicin, and low-concentration streptomycin over 6.5 months. Colored bars indicate standard error of the mean. Asterisks denote adjusted level of statistical significance from post-hoc comparisons (no significance: ns; p < 0.05: *; p < 0.01: **; p < 0.001: ***; p < 0.0001: ****).

Antibiotic literature review results_ Antibiotics are organized alphabetically within their respective classes_ Stability in solution and in agar at 4 °C is given, then resistance in Enterococcus and Staphylococcus is briefly described_

ClassAntibioticStability in solution at 4°CStability in agar plates at 4°CEnterococcus resistance
Staphylococcus resistance
Low-levelHigh-level
AminoglycosideAmikacin60 days (WHO, 2014)UnknownIntrinsic (Chow, 2000)Can be acquired (Chow, 2000)Can be acquired (Gold et al., 2014)
Gentamicin4 weeks (Bastani et al., 2005)UnknownIntrinsic (Hollenbeck and Rice, 2012)Can be acquired (Hollenbeck and Rice, 2012)Intrinsic (Cattoir, 2016; Domínguez et al., 2002; Vestergaard et al., 2016)
Kanamycin12 months (US Biological Life Sciences, 2023)30+ days (Ryan et al., 1970)Intrinsic (Hollenbeck and Rice, 2012)UnknownObserved, unknown if intrinsic or acquired (Domínguez et al., 2002)
Streptomycin2 weeks (Millipore Sigma, 2021)30+ days (Ryan et al., 1970)Intrinsic (Hollenbeck and Rice, 2012)Can be acquired (Chow, 2000)Intrinsic (Jensen and Lyon, 2009)
Tobramycin96 hours (GlobalRPH, 2001)UnknownIntrinsic (Hollenbeck and Rice, 2012)UnknownObserved, unknown if intrinsic or acquired (Domínguez et al., 2002)
Beta-lactamCephalosporin7–30 days (Xu et al., 2002)UnknownIntrinsic (Hollenbeck and Rice, 2012)Intrinsic (Hollenbeck and Rice, 2012)Intrinsic (Bruns and Keppeler, 1980)
Carbapenem (beta-lactam with hydrolase resistance)Ertapenem24 hours (GlobalRPH, 2008)UnknownIntrinsic (Werth, 2022)UnknownCan be acquired (Gesser et al., 2004; Ghosh and Banerjee, 2016)
LincomycinClindamycin32 days (GlobalRPH, 2007)UnknownIntrinsic (Hollenbeck and Rice, 2012)UnknownCan be acquired (Bastani et al., 2005)
MacrolideErythromycin7 days (Al-Ramahi et al., 2015)30+ days (Ryan et al., 1970)Can be acquired (Kristich et al., 2014)Can be acquired (Kristich et al., 2014)Can be acquired (Miklasińska-Majdanik, 2021)
Penicillin-like beta-lactamsAmoxicillin10 days (Al-Ramahi et al., 2015)UnknownCan be acquired (Conceição et al., 2012)Can be acquired (Conceição et al., 2012)Intrinsic (Bruns and Keppeler, 1980)
Ampicillin3 weeks (Sigma-Aldrich, 2016)7 days (Ryan et al., 1970)Can be acquired (Hollenbeck and Rice, 2012; Kristich et al., 2014)Can be acquired (Hollenbeck and Rice, 2012; Kristich et al., 2014)Intrinsic (Bruns and Keppeler, 1980)
Penicillin2 weeks (21)7 days (Ryan et al., 1970)Intrinsic (Hollenbeck and Rice, 2012)UnknownIntrinsic (Bruns and Keppeler, 1980; Lowy, 2003)
TetracyclineTetracycline2 weeks (Barrick Lab, 2022)30+ days (Ryan et al., 1970)Can be acquired (Wilcks et al., 2005)Can be acquired (Wilcks et al., 2005)Can be acquired (Grossman, 2016)
Language: English
Page range: 43 - 52
Published on: Jul 7, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2026 Jordan McKaig, Christopher Carr, published by American Society for Gravitational and Space Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.