Figure 1.
Figure 2.
Comparisons between the quantifications of DNA from the bacterial consortium in lunar regolith after suspension, before and after purification, measured in Nanodrop photometer and in Qubit fluorometer_
| Sample | Nanophotometer Analysis | Qubit Fluorometer | ||||||
|---|---|---|---|---|---|---|---|---|
| Before purification | After purification | Before purification | After purification | |||||
| Purity | Concentration | Purity | Concentration | Concentration | Concentration | |||
| A260/280 | A260/230 | ng/uL | A260/280 | A260/230 | ng/uL | ng/uL | ng/uL | |
| BC1 | 2.6 | 0.47 | 97.6 | 2.27 | 1.44 | 23.8 | 10.1 | 19.9 |
| BC2 | 2.6 | 0.5 | 100.2 | 2.26 | 1.6 | 24.3 | 15.2 | 22.2 |
| BC3 | 2.58 | 0.5 | 76.2 | 2.66 | 1.39 | 8 | 6.82 | 11.7 |
| Average | 2.6 | 0.5 | 91.3 | 2.4 | 1.5 | 18.7 | 10.7 | 17.9 |
Results of metagenomic amplicon sequencing 16S rDNA of samples of Bacterial Consortium in lunar regolith obtained after the protocol described_
| Sample | RawPE | Combined | Qualified | Nochime | Base(nt) | Avglen(nt) | GC | Q20 | Q30 |
|---|---|---|---|---|---|---|---|---|---|
| BC1 | 206539 | 204860 | 199906 | 192558 | 80258689 | 416.80 | 55.52% | 98.86% | 95.82% |
| BC2 | 204822 | 203775 | 199144 | 195115 | 80416946 | 412.15 | 56.13% | 98.95% | 96.22% |
| BC3 | 214590 | 213248 | 207797 | 204108 | 84221902 | 412.63 | 56.04% | 98.83% | 95.69% |
Estimates of microbial biomass and DNA yield per gram of regolith derived from the bacterial suspension approach_
| Metric | Value | Notes |
|---|---|---|
| CFU per mL | 1.16 × 107 | bacterial suspension approach |
| Total suspension volume (mL) | 90 | 10 g regolith |
| Regolith mass used for CFU estimation (g) | 10 | initial mass |
| Cells per gram of regolith (cells/g) | 1.04 × 108 | CFU/mL × volume ÷ mass |
| Regolith mass used for DNA extraction (g) | 30 | per sample |
| DNA yield BC1 (ng/g) | 26.5 | 796 ng / 30 g |
| DNA yield BC2 (ng/g) | 29.6 | 888 ng / 30 g |
| DNA yield BC3 (ng/g | 15.6 | 468 ng / 30 g |
| Mean ± SD DNA (ng/g) | 23.9 ± 7.4 | n=3 |