Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.
Figure 6.
Figure 7.
Recipe for microbial ersatz MTN featuring final component concentrations_
| Component | Concentration | Component | Concentration | ||
|---|---|---|---|---|---|
| Propylene Glycol | 8.971 | μL/L | Benzyl Alcohol | 6.075 | μL/L |
| Ethanol | 44.946 | μL/L | Diethylphthalate | 2.01 | μL/L |
| Acetone | 25.799 | μL/L | Trimethyl Silanol | 0.635 | μL/L |
| 2-(2-butoxyethoxy) Ethanol | 2.356 | μL/L | Benzothiazole | 0.21 | μL/L |
| N, N-Dimethylformamide | 1.486 | μL/L | 2-Ethyl-1-Hexanol | 0.48 | μL/L |
| 2-Ethoxyethanol | 1.303 | μL/L | Decamethylcyclopentasiloxane | 1.03 | μL/L |
| 1-Methyl-2-Pyrrolidinone | 0.782 | μL/L | Dodecamethylcyclohexasiloxane | 10 | μL/L |
| 2-Propanol | 0.896 | μL/L | Octamethylcyclotetrasiloxane | 1.02 | μL/L |
| 1-Propanol | 0.876 | μL/L | Dimethoxydimethylsilane | 35.1 | μL/L |
| 4-Ethylmorpholine | 2.265 | μL/L | Calcium Sulfate | 1.885 | mg/L |
| Formic Acid | 27.489 | μL/L | Dimethyl Sulfone | 0.205 | mg/L |
| Lactic Acid | 8.55 | μL/L | Hexamethylcyclotrisiloxane | 1.02 | mg/L |
| Benzoic Acid | 3.015 | mg/L | Monobasic Potassium phosphate | 0.835 | mg/L |
| Caprolactam | 1.111 | mg/L | Magnesium chloride hexahydrate | 0.397 | mg/L |
| Urea | 1.899 | mg/L | Manganese chloride tetrahydrate | 0.319 | mg/L |
| Zinc (II) Acetate dihydrate | 1.425 | mg/L | Ferric chloride | 0.129 | mg/L |
| Nickel (II) Acetate tetrahydrate | 0.518 | mg/L | Boric acid | 0.268 | mg/L |
| Acetic Acid | 32.088 | μL/L | Cobalt chloride hexahydrate | 0.004 | mg/L |
| Ammonium Bicarbonate | 101.457 | mg/L | Sodium molybdate dihydrate | 0.016 | mg/L |
| Sodium Fluoride | 0.88 | mg/L | |||
| Potassium Iodide | 0.021 | mg/L | |||
P-values for statistical significance of individual factors comparing viable cell densities of the accumulated biofilms_ Bolded values represent those less than 0_05, indicating the factor had a significant impact on viable biofilm accumulation of the organism_ Table key: No P: no phosphorus medium; FS: full strength medium; AgF: silver fluoride biocide; Inc: Inconel; Tef: Teflon; SL: Sher-Loxane 800 coating; and Unc: uncoated_
| Factor | B. contaminans | C. mutabilis | M. organophilum | R. insidiosa | R2A (whole biofilm) |
|---|---|---|---|---|---|
| Medium (No P < FS) | <0.00005 | 0.0556 | <0.00005 | <0.00005 | <0.00005 |
| Biocide (AgF < No biocide) | <0.00005 | <0.00005 | <0.00005 | <0.00005 | <0.00005 |
| Material (Inc < Tef) | 0.0269 | 0.0003 | 0.0002 | 0.0497 | 0.9930 |
| Coating (SL < Unc) | <0.00005 | <0.00005 | <0.00005 | <0.00005 | <0.00005 |
P-values for statistical significance of individual fixed factors comparing viable cell densities of the planktonic cells in the reactor fluid_ Bolded values are less than 0_05, indicating the factor had a significant impact on the viable planktonic density of the organism_ Table key: No P: no phosphorus medium; FS: full strength medium; AgF: silver fluoride biocide; SL: Sher-Loxane 800 coating; and Unc: uncoated_
| Factor | B. contaminans | C. mutabilis | M. organophilum | R. insidiosa | R2A counts (total planktonic) |
|---|---|---|---|---|---|
| Medium (No P < FS) | 0.0011 | 0.9320 | 0.0013 | 0.0003 | 0.0049 |
| Biocide (AgF < No biocide) | <0.00005 | 0.0110 | 0.0001 | <0.00005 | <0.00005 |
| Coating (SL < Unc) | 0.2827 | 0.0042 | 0.0464 | <0.00005 | 0.0009 |
Selective agar used for microbial enumeration of planktonic and biofilm samples_
| Organism enumerated | Agar |
|---|---|
| B. contaminans | Pseudomonas isolation agar |
| C. mutabilis | Sabouraud dextrose agar, pH lowered to 3 |
| M. organophilum | Methylobacterium agar (Atlas, 2010) with amphotericin B (7.5 μg/mL) |
| R. insidiosa | R2A with chloramphenicol (20 μg/mL) and amphotericin B (7.5 μg/mL) |
| No isolation, general counts | R2A |