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Beware of Fixation—It Might Affect Your Experiments Cover

Beware of Fixation—It Might Affect Your Experiments

Open Access
|Jul 2020

References

  1. Abbaraju NV, Cai Y, Rees BB (2011) Protein recovery and identification from the gulf killifish, Fundulus grandis: comparing snap-frozen and RNAlater® preserved tissues. Proteomics11: 4257–4261
  2. Almoguera C, Personat JM, Prieto-Dapena P, Jordano J (2015) Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta242: 461–475
  3. Botling J, Edlund K, Segersten U, Tahmasebpoor S, Engström M, Sundström M, Malmström PU, Micke P (2009) Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue. Diagnostic Molecular Pathology18: 44–52
  4. Cha J-Y, Ahn G, Kim JY, Kang SB, Kim MR, Su'udi M, Kim W-Y, Son D (2013) Structural and functional differences of cytosolic 90-kDa heat-shock proteins (Hsp90s) in Arabidopsis thaliana. Plant Physiology and Biochemistry70: 368–373
  5. Cheng J, Cheng X, Wang L, He Y, An C, Wang Z, Zhang H (2015) Physiological characteristics of seed reserve utilization during the early seedling growth in rice. Brazilian Journal of Botany38: 751–759
  6. Choudhary MK, Nomura Y, Shi H, Nakagami H, Somers DE (2016) Circadian profiling of the Arabidopsis proteome using 2D-DIGE. Frontiers in Plant Science7: 1007
  7. D'Alessandro S, Golin S, Hardtke CS, Lo Schiavo F, Zottini M (2015) The co-chaperone p23 controls root development through the modulation of auxin distribution in the Arabidopsis root meristem. Journal of Experimental Botany66: 5113–5122
  8. Dantán-González E, Rosenstein Y, Quinto C, Sánchez F (2001) Actin monoubiquitylation is induced in plants in response to pathogens and symbionts. Molecular Plant-Microbe Interactions14: 1267–1273
  9. Dunaeva M, Adamska I (2001) Identification of genes expressed in response to light stress in leaves of Arabidopsis thaliana using RNA differential display. European Journal of Biochemistry268: 5521–5529
  10. Dunmire V, Wu C, Symmans WF, Zhang W (2002) Increased yield of total RNA from fine-needle aspirates for use in expression microarray analysis. BioTechniques33: 890–896
  11. Fajardy I, Moitrot E, Vambergue A, Vandersippe-Millot M, Deruelle P, Rousseaux J (2009) Time course analysis of RNA stability in human placenta. BioMed Central (BMC) Molecular Biology10: 21
  12. Fengler S, Spirer I, Neef M, Ecke M, Nieselt K, Hampp R (2015) A whole-genome microarray study of Arabidopsis thaliana semisolid callus cultures exposed to microgravity and nonmicrogravity related spaceflight conditions for 5 days on board of Shenzhou 8. BioMed Research International547495
  13. Ferl RJ, Koh J, Denison F, Paul A-L (2015) Spaceflight induces specific alterations in the proteomes of Arabidopsis. Astrobiology15: 32–56
  14. Ferl RJ, Zupanska A, Spinale A, Reed D, Manning-Roach S, Guerra G, Cox DR, Paul A-L (2011) The performance of KSC Fixation Tubes with RNALater for orbital experiments: a case study in ISS operations for molecular biology. Advances in Space Research48: 199–206
  15. Garcia L, Welchen E, Gey U, Arce AL, Steinebrunner I, Gonzalez DH (2016) The cytochrome c oxidase biogenesis factor AtCOX17 modulates stress responses in Arabidopsis. Plant Cell and Environment39: 628–644
  16. Gilliland LU, Pawloski LC, Kandasamy MK, Meagher RB (2003) Arabidopsis actin gene ACT7 plays an essential role in germination and root growth. Plant Journal33: 319–328
  17. González-Verdejo CI, Die JV, Nadal S, Jiménez-Marín A, Moreno MT, Román B (2008) Selection of housekeeping genes for normalization by real-time RT-PCR: analysis of Or-MYB1 gene expression in Orobanche ramosa development. Analytical Biochemistry379: 176–181
  18. Grotzer MA, Patti R, Geoerger B, Eggert A, Chou TT, Phillips PC (2000) Biological stability of RNA isolated from RNAlater-treated brain tumor and neuroblastoma xenografts. Medical and Pediatric Oncology34: 438–442
  19. Gupta V, Holets-Bondar L, Roby KF, Enders G, Tash JS (2015) A tissue retrieval and postharvest processing regimen for rodent reproductive tissues compatible with long-term storage on the International Space Station (ISS) and post-flight biospecimen sharing program. BioMed Research International475935
  20. Jiang SY, Chi YH, Wang JZ, Zhou JX, Cheng YS, Zhang BL, Ma A, Vanitha J, Ramachandran S (2015) Sucrose metabolism gene families and their biological functions. Scientific Reports5: 17583
  21. Kittang A-I, Kvaløy B, Winge P, Iversen T-H (2010) Ground testing of Arabidopsis preservation protocol for the microarray analysis to be used in the International Space Station (ISS) European Modular Cultivation System (EMCS) Multigen-2 experiment. Advances in Space Research46: 1249–1256
  22. Kwon T, Sparks JA, Nakashima J, Allen SN, Tang Y, Blancaflor EB (2015) Transcriptional response of Arabidopsis seedlings during spaceflight reveals peroxidase and cell wall remodeling genes associated with root hair development. American Journal of Botany102: 21–35
  23. Lafarga V, Cuadrado A, Lopez de Silanes I, Bengoechea R, Fernandez-Capetillo O, Nebreda AR (2009) p38 mitogen-activated protein kinase- and HuR-dependent stabilization of p21(Cip1) mRNA mediates the G(1)/S checkpoint. Molecular and Cellular Biology29: 4341–4351
  24. Liu A, Chen S, Wang M, Wang Z, Zheng C, Zhao P, Guo D, Ahammed GJ (2015) Silencing of mitochondrial uncoupling protein gene aggravates chilling stress by altering mitochondrial respiration and electron transport in tomato. Acta Physiologiae Plantarum37: 223
  25. Mehdi Khanlou K, Van Bockstaele E (2012) A critique of widely used normalization software tools and an alternative method to identify reliable reference genes in red clover (Trifolium pratense L.). Planta236: 1381–1393
  26. Millar KD, Johnson CM, Edelmann RE, Kiss JZ (2011) An endogenous growth pattern of roots is revealed in seedlings grown in microgravity. Astrobiology11: 787–797
  27. Ndimba B, Rafudeen S, Gehring C, Meyer Z, Simon W, Chivasa S, Slabas A (2005) Proteomic identification of an hsp70.1 protein induced in Arabidopsis cells following hyperosmotic stress trea®ents. South African Journal of Science101: 449–453
  28. Nsubuga AM, Robbins MM, Roeder AD, Morin PA, Boesch C, Vigilant L (2004) Factors affecting the amount of genomic DNA extracted from ape faeces and the identification of an improved sample storage method. Molecular Ecology13: 2089–2094
  29. Ohama N, Kusakabe K, Mizoi J, Zhao H, Kidokoro S, Koizumi S, Takahashi F, Ishida T, Yanagisawa S, Shinozaki K, Yamaguchi-Shinozaki K (2016) The transcriptional cascade in the heat stress response of Arabidopsis is strictly regulated at the level of transcription factor expression. Plant Cell28: 181–201
  30. Páska C, Bögi K, Szilák L, Tokés A, Szabó E, Sziller I, Rigó Jr J, Sobel G, Szabó I, Kaposi-Novák P, Kiss A, Schaff Z (2004) Effect of formalin, acetone, and RNAlater fixatives on tissue preservation and different size amplicons by real-time PCR from paraffin-embedded tissue. Diagnostic Molecular Pathology13: 234–240
  31. Paul A-L, Levine HG, McLamb W, Norwood KL, Reed D, Stutte GW, Wells HW, Ferl RJ (2005b) Plant molecular biology in the space station era: utilization of KSC fixation tubes with RNAlater. Acta Astronautica56: 623–628
  32. Paul A-L, Popp MP, Gurley WB, Guy C, Norwood KL, Ferl RJ (2005a) Arabidopsis gene expression patterns are altered during spaceflight. Advances in Space Research36: 1175–1181
  33. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research29: e45
  34. Pietsch J, Ma X, Wehland M, Aleshcheva G, Schwarzwälder A, Segerer J, Birlem M, Horn A, Bauer J, Infanger M, Grimm D (2013) Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 space mission. Biomaterials34: 7694–7705
  35. Riesgo A, Pérez-Porro AR, Carmona S, Leys SP, Giribet G (2012) Optimization of preservation and storage time of sponge tissues to obtain quality mRNA for next-generation sequencing. Molecular Ecology Resources12: 312–322
  36. Stoll B, Stoll K, Steinhilber J, Jonietz C, Binder S (2013) Mitochondrial transcript length polymorphisms are a widespread phenomenon in Arabidopsis thaliana. Plant Molecular Biology81: 221–233
  37. Sun YG, Wang B, Jin SH, Qu XX, Li YJ, Hou BK (2013) Ectopic expression of Arabidopsis hlycosyltransferase UGT85A5 enhances salt stress tolerance in tobacco. PLoS One8: e59924
  38. Wang SS, Sherman ME, Rader JS, Carreon J, Schiffman M, Baker CC (2006) Cervical tissue collection methods for RNA preservation: comparison of snap-frozen, ethanol-fixed, and RNAlater-fixation. Diagnostic Molecular Pathology15: 144–148
  39. Wang X, Yan B, Shi M, Zhou W, Zekria D, Wang HZ, Kai G (2016) Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress. Protoplasma253: 637–645
  40. Wang Z, Chen Y, Fang H, Shi H, Chen K, Zhang Z, Tan X (2014) Selection of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in Brassica napus under various stress conditions. Molecular Genetics and Genomics289: 1023–1035
  41. Wu X, Brewer G (2012) The regulation of mRNA stability in mammalian cells: 2.0. Gene500: 10–21
  42. Xiao D, Zhang N-W, Zhao J-J, Bonnema G, Hou X-L (2012) Validation of reference genes for real-time quantitative PCR normalization in non-heading Chinese cabbage. Functional Plant Biology39: 342–350
  43. Zupanska AK, Denison FC, Ferl RJ, Paul A-L (2013) Spaceflight engages heat shock protein and other molecular chaperone genes in tissue culture cells of Arabidopsis thaliana. American Journal of Botany100: 235–248
Language: English
Page range: 47 - 57
Published on: Jul 18, 2020
Published by: American Society for Gravitational and Space Research
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year

© 2020 Myoung-Ryoul Park, Karl H. Hasenstein, published by American Society for Gravitational and Space Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.