Phthalates in breast milk

Alžbeta Jarošová; Vendula Kloudová

doi:10.2478/fv-2026-0030

Phthalates in breast milk

Folia Veterinaria

Volume 70 (2026): Issue 3 (September 2026)

By: Alžbeta Jarošová and Vendula Kloudová

Open Access

|Sep 2026

Abstract

Breast milk collected from the 10th day through the 5th month after delivery was analyzed for DEHP (di-2-ethylhexyl phthalate) and DBP (di-butyl phthalate) content. Dibutyl phthalate concentrations ranged from 5.17 μg/L to 2.81 μg/L, and di-2-ethylhexyl phthalate was not detected in breast milk. Statistical analysis showed that the time factor influenced phthalate levels in breast milk, with a gradual decrease observed. The expected trend of increasing phthalate levels with the duration of breastfee-ding was not statistically confirmed. In none of the monthly milk samples did phthalate intake exceed the tolerable daily intake (TDI) for infants. The estimated daily intake of dibutyl phthalate for infants ranged from 0.848 μg/kg to 0.355 μg/kg of body weight. The tolerable daily intake for DBP is set at 10 μg/kg of body weight per day and for DEHP at 50 μg/kg of body weight per day.

References

1. Sokołowski, A., Konczak, M., Oleszczuk, P., Gao, Y., Czech, B., 2024: Environmental and food contamination by phthalic acid esters (PAEs): Overview. Water Air Soil Pollut., 235, 5. DOI: 10.1007/s11270-024-07121-5
Search in Google Scholar Back to article
2. Tan, H., Yang, L., Huang, D., Chen, H., Yang, Y., Chen, D., 2023: Contamination of baby foods by plastic additives: A pilot screening study. Environ. Sci. Technol. Lett., 10, 4, 322–327. DOI: 10.1021/acs.estlett.3c00100
Search in Google Scholar Back to article
3. Haji Harunarashid, N. Z. I., Lim, L. H., Harunsani, M. H., 2017: Phthalate sample preparation methods and analysis in food and food packaging: A review. Food Anal. Methods, 10, 12, 3790–3814. DOI: 10.1007/s12161-017-0938-7
Search in Google Scholar Back to article
4. Zhang, Y., Chen, L.-B., Shen, H.-Y., Wu, Z.-C., Zhu, N.-Z., Gao, C.-J., Guo, Y., 2025: Occurrence, migration behavior, and environmental burden of phthalate esters in flooring materials used in newly renovated chinese homes. Toxics, 13, 7, 517. DOI: 10.3390/toxics13070517
Search in Google Scholar Back to article
5. Fan, J.-C., Ren, R., He, H.-L., Jin, Q., Wang, S.-T., 2020: Determination of phthalate esters in breast milk before and after frozen storage in milk storage bags. Food Addit. Contam. Part A, 37, 11, 1897–1905. DOI: 10.1080/19440049.2020.1815862
Search in Google Scholar Back to article
6. Wang, Y., Zhu, H., Kannan, K., 2019: A review of biomonitoring of phthalate exposures. Toxics, 7, 2, 21. DOI: 10.3390/toxics7020021
Search in Google Scholar Back to article
7. Khishdost, M., Dobaradaran, S., Goudarzi, G., Takdastan, A., Babaei, A. A., 2023: Contaminant occurrence, distribution and ecological risk assessment of phthalate esters in the Persian Gulf. PLoS One, 18, 7, e0287504. DOI: 10.1371/journal.pone.0287504
Search in Google Scholar Back to article
8. Dutta, S., Haggerty, D., Rappolee, D. A., Ruden, D. M., 2020: Phthalate exposure and long-term epigenomic consequences: A review. Front. Genet., 11, 405. DOI: 10.3389/fgene.2020.00405
Search in Google Scholar Back to article
9. Wang, Y., Qian, H., 2021: Phthalates and their impacts on human health. Healthcare, 9, 5, 603. DOI: 10.3390/health-care9050603
Search in Google Scholar Back to article
10. Almeida-Toledano, L., Navarro-Tapia, E., Sebastiani, G., Ferrero-Martínez, S., Ferrer-Aguilar, P., García-Algar, Ó., et al., 2024: Effect of prenatal phthalate exposure on fetal development and maternal/neonatal health consequences: A systematic review. Sci. Total Environ., 950, 175080. DOI: 10.1016/j.scitotenv.2024.175080
Search in Google Scholar Back to article
11. Ito, Y., Kamijima, M., Nakajima, T., 2019: Di(2-ethylhexyl) phthalate-induced toxicity and peroxisome proliferator-activated receptor alpha: A review. Environ. Health Prev. Med., 24, 47. DOI: 10.1186/s12199-019-0802-z
Search in Google Scholar Back to article
12. Fang, H., Wang, H., Zeng, C., Fu, H., Zhao, B., Liu, A., et al., 2022: A preliminary cumulative risk assessment of Diethylhexyl phthalate and Dibutyl phthalate based on the inhibition of embryonic development via the PPARγ pathway. Toxicol. In Vitro, 84, 105430. DOI: 10.1016/j. tiv.2022.105430
Search in Google Scholar Back to article
13. Kim, S., Lee, J., Park, J., Kim, H.-J., Cho, G., Choi, K., et al., 2015: Concentrations of phthalate metabolites in breast milk in Korea: Estimating exposure to phthalates and potential risks among breast-fed infants. Sci. Total Environ., 508, 1, 13–19. DOI: 10.1016/j.scitotenv.2014.11.019
Search in Google Scholar Back to article
14. Cao, X.-L., Sparling, M., Zhao, W., Arbuckle, T., 2021: GC-MS Analysis of phthalates and di-(2-thylhexyl) adipate in canadian human milk for exposure assessment of infant population. J. AOAC Int., 104, 1, 98–102. DOI: 10.1093/jaoacint/qsaa108
Search in Google Scholar Back to article
15. Fromme, H., Gruber, L., Seckin, E., Raab, U., Zimmer-mann, S., Kiranoglu, M., et al., 2011: Phthalates and their metabolites in breast milk — Results from the bavarian monitoring of breast milk (BAMBI). Environ. Int., 37, 4, 715–722. DOI: 10.1016/j.envint.2011.02.008
Search in Google Scholar Back to article
16. Loughnan, M., MehringLeDoare, K., 2020: Breast Milk Composition. In: Encyclopedia of Evolutionary Psychological Science. DOI: 10.1007/978-3-319-16999-6_828-1
Search in Google Scholar Back to article
17. Muro-Valdez, J. C., Meza-Rios, A., Aguilar-Uscanga, B. R., Lopez-Roa, R. I., Medina-Díaz, E., Franco-Torres, E. M., et al., 2023: Breastfeeding-related health benefits in children and mothers: Vital organs perspective. Medicina, 59, 9, 1535. DOI: 10.3390/medicina59091535
Search in Google Scholar Back to article
18. Bi, M., Liu, W., Luan, X., Li, M., Liu, M., Liu, W., et al., 2021: Production, use, and fate of phthalic acid esters for polyvinyl chloride products in China. Environ. Sci. Technol., 55, 20, 13980–13989. DOI: 10.1021/acs.est.1c02374
Search in Google Scholar Back to article
19. Yi, D. Y., Kim, S. Y., 2021: Human breast milk composition and function in human health: From nutritional components to microbiome and microRNAs. Nutrients, 13, 9, 3094. DOI: 10.3390/nu13093094
Search in Google Scholar Back to article
20. Gajdůšková, V., Černá, M., Malý, M., Šmíd, J., 1996: Concentrations of polychlorinated biphenyls and chlorinated pesticides in human breast milk-case study. Cent. Eur. J. Public Health, 4, 2, 110–114. ISSN 1210-7778.
Search in Google Scholar Back to article
21. ČSN ISO 1211, 2011: Mléko – Stanovení obsahu tuku – Vážková metoda (Referenční meto-da). Praha: Úřad pro technickou normalizaci, metrologii a státní zkušebnictví, 24 s. Třídící znak 57 0534.
Search in Google Scholar Back to article
22. Zhu, J., Phillips, S. P., Feng, Y.-L., Yang, X., 2006: Phtha-late esters in human milk: Concentration variations over a 6-month postpartum time. Environ. Sci. Technol., 40, 17, 5276–5281. DOI: 10.1021/es060356w
Search in Google Scholar Back to article
23. Main, K. M., Mortensen, G. K., Kaleva, M. M., Boisen, K. A., Damgaard, I. N., Chellakooty, M., et al., 2006: Human breast milk contamination with phthalates and alterations of endogenous reproductive hormones in infants three months of age. Environ. Health Perspect., 114, 2, 270–276. DOI: 10.1289/ehp.8075
Search in Google Scholar Back to article
24. Fan, J.-C., Ren, R., Jin, Q., He, H.-L., Wang, S.-T., 2019: Detection of 20 phthalate esters in breast milk by GC-MS/MS using QuEChERS extraction method. Food Addit. Contam. Part A, 36, 10, 1551–1558. DOI: 10.1080/19440049.2019.1646435
Search in Google Scholar Back to article
25. Liu, Y., Xiao, M., Huang, K., Cui, J., Liu, H., Yu, Y., et al., 2024: Phthalate metabolites in breast milk from mothers in Southern China: Occurrence, temporal trends, daily intake, and risk assessment. J. Hazard. Mater., 464, 132895. DOI: 10.1016/j.jhazmat.2023.132895
Search in Google Scholar Back to article
26. Kim, J. H., Kim, D., Moon, S.-M., Yang, E. J., 2020: Associations of lifestyle factors with phthalate metabolites, bisphenol A, parabens, and triclosan concentrations in breast milk of Korean mothers. Chemosphere, 249, 126149. DOI: 10.1016/j.chemosphere.2020.126149
Search in Google Scholar Back to article
27. EFSA, 2005: Opinion of the scientific panel on food additives, flavourings, processing aids and materials in contact with food (AFC) related to di-butylphthalate (DBP) for use in food contact materials. EFSA J., 3, 9, 242. DOI: 10.2903/j. efsa.2005.242
Search in Google Scholar Back to article
28. Silano, V., Barat Baviera, J. M., Bolognesi, C., Chesson, A., Cocconcelli, P. S., Crebelli, R., et al., 2019: Update of the risk assessment of di‐butylphthalate (DBP), butyl‐benzyl‐phthalate (BBP), bis(2‐ethylhexyl)phthalate (DEHP), di‐ isononylphthalate (DINP) and di‐isodecylphthalate (DIDP) for use in food contact materials. EFSA J., 17, 12, e05838. DOI: 10.2903/j.efsa.2019.5838 DOI: 10.2478/fv-2026-0021
Search in Google Scholar Back to article

Articles in this issue

DOI: https://doi.org/10.2478/fv-2026-0030 | Journal eISSN: 2453-7837 | Journal ISSN: 0015-5748

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Language: English

Page range: 76 - 84

Submitted on: Jun 24, 2026

Accepted on: Sep 6, 2026

Published on: Sep 30, 2026

Published by: The University of Veterinary Medicine and Pharmacy in Košice

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

breast milk,

liquid chromatography,

phthalic acid esters,

Related subjects:

Microbiology and virology,

Medicine,

Veterinary medicine

© 2026 Alžbeta Jarošová, Vendula Kloudová, published by The University of Veterinary Medicine and Pharmacy in Košice
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 70 (2026): Issue 3 (September 2026)