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Protective Effects of Piroxicam Against Anthracene-Induced Haematological and Biochemical Alteration in Female Rats Cover

Protective Effects of Piroxicam Against Anthracene-Induced Haematological and Biochemical Alteration in Female Rats

Open Access
|Mar 2026

References

  1. 1. Lindsey, P. A., Nyirenda, V. R., Barnes, J. I., Becker, M. S., McRobb, R., Tambling, C. J., et al., 2014: Underperformance of African protected area networks and the case for new conservation models: insights from Zambia. Plos One, 9, 5, e94109. DOI: 10.1371/journal.pone.0094109
  2. 2. IARC, 2004: Tobacco smoke and involuntary smoking. Monographs on the Evaluation of Carcinogenic Risk to Humans, 83, 33–1187.
  3. 3. Collin, G., H ö ke, H., Talbiersky, J., 2006: Anthracene. In Ullmann ’ s Encyclopedia of Industrial Chemistry, Wiley-VCH, Weinheim. DOI: 10.1002/14356007.a02_343. pub2
  4. 4. Verschueren, K., Marcoen, A., Schoefs, V., 1996: The internal working model of the self, attachment, and competence in five-year-olds. Child Dev., 67, 5, 2493–2511.DOI: 10.2307/1131636
  5. 5. Rao, P-S. 2014: Anthracene. In Encyclopaedia of Toxicology, 3rd ed., pp. 260–261.
  6. 6. Abdul Lateef, E. N., Hussein, M. A., Mustafa, S. R., Taen, M. M., Ali, S. A., Saheab, N., 2021: Toxicity of anthracene on the function of the liver and kidney of the common carp cypricus carpio. Egyptian J. Aquat. Biol. Fish, 25, 3, 3, 831–840. DOI: 10.21608/EJABF.2021.182302
  7. 7. Dey, S., Samanta, P., Mondal, N. S., Kole, D., Mandal, A., Patra, A., Ghosh, A. R., 2019: Dose specific responses of Anabas testudineus (Bloch) to anthracene (PAH): haemato-logical and biochemical manifestation. Emerg. Contamin., 5, 232–239. DOI: 10.1016/j.emcon.2019.07.001
  8. 8. Palanikumar, L., Kumaraguru, A. K., Ramakritinan, C. M., Anand, M., 2012: Biochemical response of anthracene and benzo[a]pyrene to milkfish Chanos chanos. Ecotoxicol. Environ. Saf., 75, 1, 187–197. DOI: 10.1016/j. ecoenv.2011.08.028
  9. 9. Saganuwan, S. A., Orinya, O. A. 2016: Toxico-neurological effects of piroxicam in monogastric animals. J. Exp. Neurosci., 10, 121–128. DOI: 10.4137/JEn.s40144
  10. 10. Saganuwan, S. A., Onyeyili, P. A., 2016: The paradox of human equivalent dose formula: a canonical case study of Abrus precatorius aqueous leaf extract in monogastric animals. Mac. Vet. Rev., 39, 1, 23–32. DOI: 10.1515/macvetrev-2015-0061
  11. 11. Akogwu, E. I., Saganuwan, S. A., Onyeyili, P. A., 2017: Comparative pharmacokinetics of piroxicam in male and female west African dwarf goats. Cog. Food Agric., 3, 1–8. DO I: 10.1080/23311932.2017.1294444
  12. 12. Oluchi, O. M., Chukwuebuka, N. H., Orbunde, D. K., Daniel, W. A., Aondohemba, U.V., Ieren, I. I., et al., 2024: Evaluation of Benz[a]anthracene-induced pulmonary toxicity in Rattus norvegicus. J. Toxicity Envir. Health Sci., 16, 1, 1–12. DOI: 10.5897/JTEHS2023.0517
  13. 13. Thermo Fisher Scientific, 2024: Carboplatin In: Safety Data Sheet, Health, Safety and Environment Department. pp. 1–8.
  14. 14. Saganuwan, S. A., 2019: Effects of therapeutic and toxic agents on erythrocytes of different species of animals. In Erythrocytes, Intech Open, London, pp. 176. DOI: 10.5772/intechopen.85865
  15. 15. Uzoamaka, A. M., Saganuwan, S. A., Ahur, M. V., Akande, T. 2025: Ameliorative effects of piroxicam on anthracene-induced lung toxicity in female Rattus norvegicus. Folia Vet., 69, 4, 49–63. DOI: 10.2478/fv-2025-0036
  16. 16. Saganuwan, S. A., 2012: Principle of pharmacological calculations 1st ed; Ahmadu Bello University press, pp. 528.
  17. 17. Kisani, A. I., Immaculate, A. N., Saganuwan, S. A., 2022: Comparative local anaesthetic effect of lignocaine, ligno-caine-adrenaline and water extracts of Annona muricata during castration in dogs. Adv. Trad. Med. 23, 851–861. DOI: 10.1007/s13596-022-00655-1
  18. 18. Saganuwan, S. A., Onyeyili, P. A., 2012: Haematonic and plasma expander effects of aqueous leaf extract of Abrus precalorius in Mus musculus. Comp. Clin. Pathol., 21, 1249–1255. DOI: 10.1007/s00580-011-1274-8
  19. 19. Brown, B. A., 1976: Haematology principles and procedures, 2nd ed. Lea and Fabiger, Philadelphia, pp. 56–81.
  20. 20. Cheesebrough, M., 2005: Examination of blood for malaria parasite. District Laboratory practice in tropical countries. Part 1. Cambridge University Press, Cambridge, pp. 434.
  21. 21. Talke, H., Schubert, G. E., 1965: Enzymatic urea determination in the blood and serum in the Warburg optical test. Klin. Wohcenschr., 43, 174–175.
  22. 22. Blass, K. G., Thibert, R. J., Lam, L. K., 1974: A study of mechanism of Jaff é reaction. Clin. Chem., 12, 7, 336–343. DOI: 10.1515/cclm.1974.12.7.336
  23. 23. Saganuwan, S. A., 2018: The use of body surface area for determination of age, body weight, urine creatinine, plasma creatinine, serum creatinine, urine volume and creatinine clearance: the reliable canonical method for accessing renotoxicity in animals. Comp. Clin. Pathol., 27, 1531–1536. DOI: 10.1007/s00580-018-2769-3
  24. 24. Bergemeyer, H. U., Bowers Jr., G. N., Horder, M., Moss, D. W., 1976: Provisional recommendations on IFCC methods for the measurement of catalytic concentrations of enzymes. Part 2. IFCC method for aspartate aminotransferase. Clin. Chim. Acta, 70, 2, 19–29. PMID: 954207
  25. 25. Henley, K. S., 1980: IFCC method for alanine aminotransferase. Clin. Chim. Acta, 105, 1, 155–166. DOI: 10.1016/0009-8981(80)90106-0
  26. 26. Roy, A. V., 1970: Rapid method for determining alkaline phosphatase activity in serum with thymolphthalein mono-phosphate. Clin. Chem., 16, 5, 431–436. PMID: 5443769
  27. 27. Williams, A. L. B. and Hoofnagle, J. H., 1988: Ratio of serum aspartate to alanine aminotransferase in chronic hepatitis relationship to cirrhosis. Gastroenterol., 95, 3, 734–739. DOI: 10.1016/s0016-5085(88)80022-2
  28. 28. Daniel, W. W., 2010: Biostatistics: Basic Concept and Methodology for Health Sciences, 9th ed., Wiley, New Delhi, pp. 783.
  29. 29. Zahorec, R., 2021: Neutrophil to lymphocyte ratio, past, present and future perspectives. Bratisl. Med. J., 122, 7, 474–488. DOI: 10.4149/BLL_2021_078
  30. 30. Cohen, J. A., Kaplan, M. M., 1979: The SGOT/SGPT ratio–an indicator of alcoholic liver disease. Dig. Dis. Sci., 24, 11, 835–838. DOI: 10.1007/BF01324898
  31. 31. Saganuwan, S. A., 2023: Ameliorative effects of piroxicam on perchloric acid-induced thyroid gland hormones disruption in male rats. J. King Saud Univ-Sci., 35, 5, 102661. DOI: 10.1016/j.jksus.2023.102661
  32. 32. Roeland, E. J., Fintelmann, F. J., Hilton, F., Yang, R., Whalen, E., Tarasenko, L., et al., 2024: The relationship between weight gain during chemotherapy and outcomes in patients with advanced non-small cell lung cancer. J. Cachexia Sarcopenia Muscle, 15, 3, 1030–1040. DOI: 10.1002/jcsm.13426
  33. 33. Story, A. L., Boston, S. E., Kilkenny, J. J., Singh, A., Woods, J. P., Culp, W. T. N., et al., 2017: Evaluation of weight change during carboplatin therapy in dogs with appendicular osteosarcoma. J. Vet. Intern. Med., 31, 4, 1159–1162. DOI: 10.1111/jvim.14724
  34. 34. Cheng, Y.-J., Wu, R., Cheng, M.-L., Du, J., Hu, X.-W., Yu, L., et al., 2017: Carboplatin-induced hematotoxicity among patients with non-small cell lung cancer: Analysis on clinical adverse events and drug-gene interactions. Oncotarget, 8, 19, 32228–32236. DOI: 10.18632/oncotarget.12951
  35. 35. Kasuga, I., Makino, S., Kiyokawa, H., Katoh, H., Ebihara, Y., Ohyashiki, K., 2001: Tumor-related leukocytosis as linked with poor prognosis in patient with lung carcinoma. Cancer, 92, 9, 2399–2405. DOI: 10.1002/1097-0142(20011101)92:9<;2399::aid-cncr1588>3.0.co;2-w
  36. 36. Sprague, B. L., Trentham-Dietz, A., Klein, B. E. K., Klein, R., Cruickshanks, K. J., Lee, K. E., Hampton, J. M., 2008: Physical activity, white blood cell count, and lung cancer risk in a prospective cohort study. Cancer Epidemiol. Biomarkers Prev., 17, 10, 2714–2722. DOI: 10.1158/1055-9965.EPI-08-0042
  37. 37. Wong, J. Y. Y., Bassig, B. A., Loftfield, E., Hu, W., Freedman, N. D., Ji, B., et al., 2020: White blood cell count and risk of incident lung cancer in the UK Biobank. JNCI Cancer Spect., 4, 2, 1–9. DOI: 10.1093/jncics/pkz102
  38. 38. Uribe – Querol, E., Rosales, C., 2015: Neutrophils in cancer: two sides of the same coin. J. Immunol. Res., 1, 983698, 1–21. DOI: 10.1155/2015/983698
  39. 39. Kohutek, F., Bystricky, B., 2019: Leukocytosis as a negative prognostic factor in patients with lung cancer: Which subpopulation of leucocytes is responsible? Tumor Biol. Pathol., 30, 5, 799.
  40. 40. Saganuwan, S.A., 2016: Physicochemical and structure-activity properties of piroxicam—a mini review. Comp Clin Pathol., 25, 941–945. DOI: 10.1007/s00580-016-2284-3
  41. 41. Young, B., Stewart, W., O ’ Dowd, G., 2011: Wheater’s basic pathology: a text, atlas and review of histopathology. 15th ed. International edition. pp. 174–175.
  42. 42. Campbell, M.S., Makar, G.A., 2005: Safety of short-term administration of celecoxib in decompensated cirrhosis. Hepatol., 42, 1, 238. DOI: 10.1002/hep.20744
  43. 43. Aloe, C., Wang, H., Vlahos, R., Irving, L., Steinfort, D., Bozinovski, S., 2021: Emerging and multifaceted role of neutrophils in lung cancer. Transl. Lung Cancer Res., 10, 6, 2806–2818. DOI: 10.21037/tlcr-20-760
  44. 44. Wang, Z., Chen, B., Fu, Y., Ou, C., Rong, Q., Kong, X., et al., 2022: Eosinophilia and Lung Cancer: Analysis from Real-World Data and Mendelian Randomization Study. Front. Med. (Lausanne), 9, 830754. DOI: 10.3389/fmed.2022.830754
  45. 45. Anthony, H. M., 1982: Blood basophils in lung cancer. Br. J. Cancer, 45, 2, 209–216. DOI: 10.1038/bjc.1982.35
  46. 46. Ahur, V. M., Agada, P. O., Saganuwan, S. A., 2012: Estimating the plasma and serum activity levels of aspartate aminotransferase and alanine aminotransferase, in live animals using regression model. Trends Appl. Sci. Res., 7, 9, 748–757. DOI: 10.3923/tasr.2012.748.757
  47. 47. Saganuwan, S. A., 2017: Functional chemical groups that may likely become a source for the synthesis of novel central nervous system (cns) acting drugs. Centr. Nerv. Syst. Agents Med. Chem., 17, 3, 178–186. DOI: 10.2174/187152491766 6170502153752
  48. 48. Saganuwan, S. A., 2017: Toxicity studies of drugs and chemicals in animals: an overview. Bulg. J. Vet. Med., 20, 4, 291–318. DOI: 10.15547/bjvm.983
  49. 49. Saganuwan, S. A., 2019: Chemistry and effects of brainstem acting drugs. Centr. Nerv. Syst. Agents Med. Chem., 19, 3, 180–186. DOI: 10.2174/1871524919666190620164355
  50. 50. Sedlack, A. J. H., Meyer, C., Mench, A., Winters, C., Barbon, D., Obrzut, S., Mallak, N., 2024: Essentials of theranostics: A guide for physicians and medical physicists. Radiographics, 44, 1, e230097. DOI: 10.1148/rg.230097
  51. 51. Pradhan, S., Sonkar, C. K., 2023: Theranostic in nuclear medicine: Therapy to impact patient management and secure the future in the era of precision oncology. Oncol. Radiother. 17, 12, 756–763.
  52. 52. Awuah, W. A., Ahluwalia, A., Tan, J. K., Sanker, V., Roy, S., Ben-Jaafar, A., et al., 2025: Theranostics advances in the treatment and diagnosis of neurological and neurosurgical diseases. Arch. Med. Res., 56, 1, 103085. DOI: 10.1016/j. arcmed.2024.103085
  53. 53. Alexander, S. P. H., Kelly, E., Mathie, A. A., Peters, J. A., Veale, E. L., Armstrong, J. F., et al., 2023: The concise guide to pharmacology 2023/24: Introduction and other protein targets. Brit. J. Pharmacol., 180, S2, S1–S22. DOI: 10.1111/bph.16176
  54. 54. Ekhart, C., Rodenhuis, S., Schellens, J. H. M., Beijnen, J. H., Huitema, A. D. R., 2009: Carboplatin dosing in over-weight and obese patients with normal renal function, does weight matter? Cancer Chemother. Pharmacol. 64, 1, 115–122. DOI: 10.1007/s00280-008-0856-x
  55. 55. Saganuwan, S. A., 2017: In vivo piroxicam metabolites: Possible sources for synthesis of central nervous system acting depressants. Cent. Nerv. Syst. Agents Med. Chem., 17, 3, 172–177. DOI: 10.2174/1871524917666161111093759
  56. 56. Egbewale, S. O., Kumar, A., Mokoena, M. P., Olaniran, A. O., 2025: Optimization of anthracene biodegradation by indigenous Trichoderma lixii and Talaromyces pinophilus using response surface methodology. Ecotoxicol. Environ. Saf., 289, 117431. DOI: 10.1016/j.ecoenv.2024.117431
  57. 57. Schwenk, M. H., 2024: Carboplatin dosing on the basis of renal function: 30+ years after calvert. Kidney 360, 5, 2, 271–273. DOI: 10.34067/KID.0000000000000349
  58. 58. Chmielewski, P. P., Strzelec, B., Mozdziak, P., Kempisty, B., 2025: Neutrophil-to-lymphocyte ratio as a prognostic biomarker for long-term survival in older adults at a mental health care center: a historical cohort analysis. J. Clin. Med., 14, 7, 2509. DOI: 10.3390/jcm14072509
  59. 59. Xu, M., Yang, X., Zhang, J., Liu, D., Zhang, C., Wu, M., et al. 2024: The mechanism of anthracene degradation by trylophan-2,3-dioxygenase (T23D) in Comamonas testosteroni. Chem. Biol. Interact., 393, 110950. DOI: 10.1016/j. cbi.2024.110950
  60. 60. Omar, A. M., Abdulmalik, O., El-say, K.M., Ghatge, M. S., Cyril-Olutayo, M., Paredes, S., et al., 2023: Targeted modification of furan-2-carboxaldehydes into Michael acceptor analogs yielded long-acting hemoglobin modulators with dual antisickling activities. Chem. Biol. Drug Des., 103, 1, e14371. DOI: 10.1111/cbdd.14371
  61. 61. Diaz-Uribe, C., Vallejo, W., Quinones, C., 2020: Physical-chemical study of anthracene selective oxidation by a Fe(III)- phenylporphyrin derivative. Int. J. Mol. Sci., 21, 1, 353. DOI: 10.3390/ijms21010353
  62. 62. Saganuwan, S. A, 2024: Structure-activity relationship of pharmacophores and toxicophores: the need for clinical strategy. Daru J. Pharm. Sci., 32, 2, 781–800. DOI: 10.1007/s40199-024-00525-y
  63. 63. Saganuwan, S. A, 2021: Application of modified Michaelis-Menten equations for determination of enzyme inducing and inhibiting drugs. BMC Pharmacol. Toxicol., 22, 57, 1–15. DOI: 10.1186/s40360-021-00521-x
  64. 64. de Sousa, G. F., Wlodarczyk, S.R., Monteiro, G., 2014: Carboplatin: Molecular mechanisms of action associated with chemoresistance. Braz. J. Pharm. Sci., 50, 4. 10.1590/S1984-82502014000400004
  65. 65. Kaushal, A., Verma, P., Singh, A. P., Singh, A. P., 2024: A critical review of piroxicam: From mechanism to adverse effects in clinical use. IJIRT, 11, 6, 3807–3811.
  66. 66. Mazumder, M. K., Borah, A., 2014: Piroxicam inhibits NMDA receptor-mediated excitotoxicity through allosteric inhibition of the GluN2B subunit: an in silico study elucidating a novel mechanism of action of the drug. Med. Hypotheses, 83, 6, 740–746. DOI: 10.1016/j.mehy.2014.09.031
  67. 67. Reitzer, L., 2004: Biosynthesis of glutamate, aspartate, asparagine, L-Alanine, and D-Alanine. EcoSal Plus, 1, 1, 26443364. DOI: 10.1128/ecosalplus.3.6.1.3
DOI: https://doi.org/10.2478/fv-2026-0004 | Journal eISSN: 2453-7837 | Journal ISSN: 0015-5748
Language: English
Page range: 33 - 46
Submitted on: Jun 15, 2025
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Accepted on: Dec 15, 2026
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Published on: Mar 21, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2026 Uzoamaka Maryjane Anthony, Saganuwan Alhaji Saganuwan, Victor Maskavem Ahur, Akande Titilayo, published by The University of Veterinary Medicine and Pharmacy in Košice
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.