Extracellular activity of proteases from Yarrowia lipolytica IPS21 as a function of the carbon and nitrogen source

Influence of the carbon source on the biomass and yeast Y. lipolytica IPS21 protein concentration. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparison of the biomass or total protein marked with the same letter

Carbon source	Biomass production, g g^-1 of carbohydrate substrate	Total protein, μg mL^-1
Yeast extract	0.385 ± 0.02^d	120 ± 5.20^a
Glucose	0.452 ± 0.11^aa	124 ± 4.73^a
Glycerol	0.465 ± 0.03^aa	120 ± 6.55^a
Starch	0.401 ± 0.02^d	114 ± 4.66^a
Semolina wheat	0.225 ± 0.09^a	111 ± 7.22^a
Oat	0.317 ± 0.12^b	109 ± 1.99^a
Saccharose	0.364 ± 0.09^c	130 ± 2.33^a
Pectin	0.299 ± 0.16^b	128 ± 2.98^a
Straw	0.364 ± 0.04^c	112 ± 7.66^a
Saccharose + CTLS^*	0.354 ± 0.04^c	124 ± 8.13^a
Starch + CTLS	0.320 ± 0.08^b	120 ± 5.78^a
CTLS	0.204 ± 0.09^a	110 ± 2.59^a

1* CTLS -chrome-tanned leather shavings

*CTLS - chrome-tanned leather shavings
Influence of the carbon source on the activity of the proteases of the yeast Y. lipolytica IPS21. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons of supernatants marked with the same letter

*CTLS -chrome-tanned leather shavings
Influence of the carbon source on the pH of the medium during the biological process with the yeast Y. lipolytica IPS21. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons of the medium marked with the same letter

Table 2.

Composition of amino acids in the supernatant after 48 hours of biological treatment with Y. lipolytica IPS21

Name of acid	Yeast extract medium with Yarrowia	CTLS medium with Yarrowia	Yeast extract medium without Yarrowia	CTLS medium without Yarrowia
	g 100 g^-1 of supernatant
Aspartic acid	0.03	0.22	0.07	0.13
Threonine	<0.02	0.05	0.07	0.04
Serine	<0.02	0.10	<0.02	0.06
Glutamic acid	0.09	0.29	0.09	0.26
Proline	<0.02	0.33	0.03	0.25
Glycine	<0.02	0.55	0.02	0.37
Alanine	<0.02	0.21	0.06	0.20
Valine	<0.02	0.05	0.05	0.06
Methionine	<0.02	<0.02	<0.02	<0.02
Isoleucine	<0.02	0.19	0.03	0.05
Leucine	<0.02	0.08	0.06	0.08
Tyrosine	<0.02	0.03	0.03	0.03
Phenylalanine	<0.02	0.07	0.04	0.05
Ornithine	<0.02	<0.02	<0.02	<0.02
Gamma-Aminobutyric acid	<0.02	<0.02	<0.02	<0.02
Lysine	<0.02	0.09	0.05	0.12
Histidine	<0.02	0.03	<0.04	<0.02
Arginine	<0.02	0.17	0.03	0.11
Taurine, 2-aminoethanesulfonic acid	<0.02	<0.02	<0.02	<0.02
Hydroxyproline	<0.02	0.30	<0.02	0.17
Cysteine	<0.02	<0.02	<0.02	<0.02
Hydroxylysine	<0.02	0.04	<0.02	<0.02
Sum of amino acid content (mean value)	0.12	2.78	0.63	1.98

Table 3.

Influence of the amount of yeast extract in the medium with 0.6 g of CTLS on the biomass and protein concentration of the yeast Y. lipolytica IPS21. Values for single elements are not significantly different (P<0.05) according to Tukey’s Kramer test for multiple comparisons of biomass or the total protein marked with the same letter

Carbon sources	Biomass production, g g^-1 of carbohydrate substrate	Total protein, μg m^L-1
0.6 g yeast extract	0.205 ± 0.14^a	112.4 ± 6.55^c
0.4 g yeast extract	0.375 ± 0.16^c	89.2 ± 4.22^b
0.2 g yeast extract	0.316 ± 0.09^b	64.2 ± 4.33^a

*YE – yeast extract
Influence of the amount of yeast extract in a medium with 0.6 g of CTLS on the activity of proteases of Y. lipolytica IPS21 yeast. Values for single elements are not significantly different (P < 0.05) according to Tukey’s Kramer test for multiple comparisons

Table 4.

Metal concentrations in selected samples of biomass with Y. lipolytica IPS21 yeast or a supernatant after the process of protease production in a medium with 0.6 g of CTLS and different amounts of yeast extract. Values for single elements are not significantly different (P<0.05) according to Tukey’s Kramer test for multiple comparisons of the biomass or supernatant marked with the same letter

Metal Variant	Ca	Cr	Fe	K	Mg	Na	P	S
g kg^-1
0.2 g YE	3.16 ±	3.68 ±	0.55 ±	5.16 ±	2.44 ±	15.44 ±	16.02 ±	3.36 ±
biomass	0.21^a	0.30^a	0.02^a	0.55^a	0.13^a	1.22^a	1.45^a	1.24^a
0.4 g YE	1.92 ±	3.33 ±	0.51 ±	4.06 ±	2.12 ±	9.81 ±	19.89 ±	5.15 ±
biomass	0.11^b	0.22^a	0.02^a	0.45^b	0.18^b	1.18^b	2.66^b	0.12^b
0.2 g YE	0.10 ±	0.54 ±	0.09 ±	0.25 ±	0.05 ±	3.0 ±	0.09 ±	0.52 ±
supernatant	0.01^a	0.01^a	0.01^a	0.08^a	0.32^a	0.54^a	0.02^a	0.13^a
0.4 YE	0.10 ±	0.53 ±	0.10 ±	0.26 ±	0.06 ±	3.3 ±	0.08 ±	0.57 ±
supernatant	0.01^a	0.02^a	0.04^a	0.02^a	0.01^a	0.03^a	0.01^a	0.03^a

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DOI: https://doi.org/10.2478/ftee-2023-0046 | Journal eISSN: 2300-7354 | Journal ISSN: 1230-3666

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Language: English

Page range: 66 - 74

Published on: Dec 1, 2023

Published by: Łukasiewicz Research Network-Łódź Institute of Technology

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

biopolymers,

carbon source,

Yarrowia lipolytica,

waste,

modification of materials

Related subjects:

Business and economics,

Business management,

Business informatics,

Process management,

Industrial chemistry,

Cellulose, paper and textiles,

Polymer science and technology,

Materials sciences,

Biomaterials and natural materials

© 2023 Dorota Wieczorek, Katarzyna Miśkiewicz, Dorota Gendaszewska, Paulina Pipiak, Magdalena Lasoń-Rydel, Katarzyna Sieczyńska, Katarzyna Ławińska, published by Łukasiewicz Research Network-Łódź Institute of Technology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 31 (2023): Issue 5 (December 2023)

Extracellular activity of proteases from Yarrowia lipolytica IPS21 as a function of the carbon and nitrogen source

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Fig. 1.

Fig. 2.

Table 2.

Table 3.

Fig. 3.

Table 4.

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