Extracellular activity of proteases from Yarrowia lipolytica IPS21 as a function of the carbon and nitrogen source

Figures & Tables

MetalVariant	Ca	Cr	Fe	K	Mg	Na	P	S
g kg^-1
0.2 g YE	3.16 ±	3.68 ±	0.55 ±	5.16 ±	2.44 ±	15.44 ±	16.02 ±	3.36 ±
biomass	0.21^a	0.30^a	0.02^a	0.55^a	0.13^a	1.22^a	1.45^a	1.24^a
0.4 g YE	1.92 ±	3.33 ±	0.51 ±	4.06 ±	2.12 ±	9.81 ±	19.89 ±	5.15 ±
biomass	0.11^b	0.22^a	0.02^a	0.45^b	0.18^b	1.18^b	2.66^b	0.12^b
0.2 g YE	0.10 ±	0.54 ±	0.09 ±	0.25 ±	0.05 ±	3.0 ±	0.09 ±	0.52 ±
supernatant	0.01^a	0.01^a	0.01^a	0.08^a	0.32^a	0.54^a	0.02^a	0.13^a
0.4 YE	0.10 ±	0.53 ±	0.10 ±	0.26 ±	0.06 ±	3.3 ±	0.08 ±	0.57 ±
supernatant	0.01^a	0.02^a	0.04^a	0.02^a	0.01^a	0.03^a	0.01^a	0.03^a

Carbon source	Biomass production, g g^-1 of carbohydrate substrate	Total protein, μg mL^-1
Yeast extract	0.385 ± 0.02^d	120 ± 5.20^a
Glucose	0.452 ± 0.11^aa	124 ± 4.73^a
Glycerol	0.465 ± 0.03^aa	120 ± 6.55^a
Starch	0.401 ± 0.02^d	114 ± 4.66^a
Semolina wheat	0.225 ± 0.09^a	111 ± 7.22^a
Oat	0.317 ± 0.12^b	109 ± 1.99^a
Saccharose	0.364 ± 0.09^c	130 ± 2.33^a
Pectin	0.299 ± 0.16^b	128 ± 2.98^a
Straw	0.364 ± 0.04^c	112 ± 7.66^a
Saccharose + CTLS^*	0.354 ± 0.04^c	124 ± 8.13^a
Starch + CTLS	0.320 ± 0.08^b	120 ± 5.78^a
CTLS	0.204 ± 0.09^a	110 ± 2.59^a

Name of acid	Yeast extract medium with Yarrowia	CTLS medium with Yarrowia	Yeast extract medium without Yarrowia	CTLS medium without Yarrowia
	g 100 g^-1 of supernatant
Aspartic acid	0.03	0.22	0.07	0.13
Threonine	<0.02	0.05	0.07	0.04
Serine	<0.02	0.10	<0.02	0.06
Glutamic acid	0.09	0.29	0.09	0.26
Proline	<0.02	0.33	0.03	0.25
Glycine	<0.02	0.55	0.02	0.37
Alanine	<0.02	0.21	0.06	0.20
Valine	<0.02	0.05	0.05	0.06
Methionine	<0.02	<0.02	<0.02	<0.02
Isoleucine	<0.02	0.19	0.03	0.05
Leucine	<0.02	0.08	0.06	0.08
Tyrosine	<0.02	0.03	0.03	0.03
Phenylalanine	<0.02	0.07	0.04	0.05
Ornithine	<0.02	<0.02	<0.02	<0.02
Gamma-Aminobutyric acid	<0.02	<0.02	<0.02	<0.02
Lysine	<0.02	0.09	0.05	0.12
Histidine	<0.02	0.03	<0.04	<0.02
Arginine	<0.02	0.17	0.03	0.11
Taurine, 2-aminoethanesulfonic acid	<0.02	<0.02	<0.02	<0.02
Hydroxyproline	<0.02	0.30	<0.02	0.17
Cysteine	<0.02	<0.02	<0.02	<0.02
Hydroxylysine	<0.02	0.04	<0.02	<0.02
Sum of amino acid content (mean value)	0.12	2.78	0.63	1.98

Carbon sources	Biomass production, g g^-1 of carbohydrate substrate	Total protein, μg m^L-1
0.6 g yeast extract	0.205 ± 0.14^a	112.4 ± 6.55^c
0.4 g yeast extract	0.375 ± 0.16^c	89.2 ± 4.22^b
0.2 g yeast extract	0.316 ± 0.09^b	64.2 ± 4.33^a