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Effect of interaction between Fusarium solani and Rhizoctonia solani on damping-off and root rot disease of Tetraclinis articulata seedlings Cover

Effect of interaction between Fusarium solani and Rhizoctonia solani on damping-off and root rot disease of Tetraclinis articulata seedlings

Open Access
|Jun 2022

Figures & Tables

Figure 1

A small lesion associated with the constricted area on the stem (hypocotyl): Disease severity 1.
A small lesion associated with the constricted area on the stem (hypocotyl): Disease severity 1.

Figure 2

Brown lesion covering 25–50% of the hypocotyl length: Disease severity 2.
Brown lesion covering 25–50% of the hypocotyl length: Disease severity 2.

Figure 3

Necrosis affecting more than 50% of the hypocotyl length: Disease severity 3.
Necrosis affecting more than 50% of the hypocotyl length: Disease severity 3.

Figure 4

T. articulata seedlings taken from different treatments at 5 weeks. Note the difference between the control plant and inoculated plants: brown to black lesion with collar-constricted stem on the Rs sample; stem canker, general wilting and rotted root on the Fs sample, stunting and rotted roots on the RsFs sample. Contrary to the control plant, all inoculated seedlings showed a lack of fine roots.
T. articulata seedlings taken from different treatments at 5 weeks. Note the difference between the control plant and inoculated plants: brown to black lesion with collar-constricted stem on the Rs sample; stem canker, general wilting and rotted root on the Fs sample, stunting and rotted roots on the RsFs sample. Contrary to the control plant, all inoculated seedlings showed a lack of fine roots.

Figure 5

T. articulata seedlings under two treatments at 62 days: inoculated seedlings (RsFs treatment) and uninoculated seedlings (control).
T. articulata seedlings under two treatments at 62 days: inoculated seedlings (RsFs treatment) and uninoculated seedlings (control).

Fungal recovery (%) from epicotyl, hypocotyl, primary and lateral roots of T_ articulata seedlings 62 days post-inoculation by R_ solani and F_ solani singly or in combination_

TreatmentIsolation percentage Pi (%)

EpicotylHypocotylPrimary rootLateral roots

R. SolaniF. SolaniR. SolaniF. SolaniR. SolaniF. SolaniR. SolaniF. Solani
Rs0707056
Fs45556075
RsFs028174313422533

Effect of fungal pathogens alone and in combination on the pre-emergence and post-emergence of the damping-off disease and survival rate of T_ articulata seedlings 62 days post-inoculation_

TreatmentPre-emergence damping-off (%)*Post-emergence damping-off (%)*Survival (%)*
Control7.37 a2.62 a90 c
Rs21 b11.12 c67.87 b
Fs22.5 b7.12 b70.37 b
RsFs26.93 c19.06 d54 a

Effect of fungal pathogens alone and in combination on different growth parameters of T_ articulata seedlings 62 days post-inoculation; reduction in growth (%) in brackets_

TreatmentShoot height (cm)*No. of leaves*No. of lateral roots*Lateral root length (cm) *Primary root length (cm) *Root system total length (cm) *
Control12.04 c57.65 c17.23 b3.6 c23.46 c86.32 b
Rs7.31 a (39.29%)49.85 b (13.53%)11.57 a (32.85%)3.14 b (12.78%)21.79 b (7.12%)51.02 a (40.89%)
Fs7.66 a (36.38%)33.74 a (41.47%)11.7 a (32.1%)2.89 ab (19.72%)19.48 a (16.97%)54.58 a (36.77%)
RsFs8.64 b (28.24%)44.34 b (23.09%)11.98 a (30.47%)2.56 a (28.89%)18.25 a (22.21%)48.98 a (43.26%)

Effect of fungal pathogens alone and in combination on disease severity and disease-index (%) recorded on T_ articulata seedlings 62 days post-inoculation_

TreatmentDisease severity*Disease index (%)

Epicotyl (0–4)Hypocotyl (0–4)Primary root (0–6)Lateral roots (0–6)
Control0.58 b**0.58 a**0.87 a**0.87 a**14.5 a**
Rs0 a2.43 b3.54 b3.15 b43.04 b
Fs1.93 c2.38 b3.43 b3.62 c56.25 c
RsFs2.23 c2.76 c4.59 c4.75 d70.1 d
DOI: https://doi.org/10.2478/fsmu-2021-0018 | Journal eISSN: 1736-8723 | Journal ISSN: 1406-9954
Language: English
Page range: 166 - 175
Submitted on: Mar 28, 2021
Accepted on: Dec 31, 2021
Published on: Jun 4, 2022
Published by: Estonian University of Life Sciences
In partnership with: Paradigm Publishing Services
Publication frequency: 2 times per year

© 2022 Rachid El Haddadi, Azeddine Errifi, Soukaina Msairi, Amina Ouazzani Touhami, Allal Douira, published by Estonian University of Life Sciences
This work is licensed under the Creative Commons Attribution 4.0 License.