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Types, concentrations and durations of sterilising agents used for the sterilisation of nodal segments_
| Types of sterilising agents | Concentration (%) | Duration (min) |
|---|---|---|
| NaOCl | 5.0, 10.0, 20.0 (v/v) | 10, 20 |
| HgCI2 | 0.1, 0.5, 1.0 (w/v) | 5, 10 |
| Na-merthiolate | 0.01, 0.05, 0.5 (w/v) | 5, 10 |
| NaDCC | 0.15, 0.25, 0.35 (w/v) | 10, 20 |
Composition of the nutrient media used at different stages of micropropagation of ‘Duke 7’ rootstock_
| Different stages | Basal medium | PGR and additives | Carbohydrate source | Gelling agent |
|---|---|---|---|---|
| Establishment | Modified MS | 0.01% AC | 3% sucrose | 0.7% agar |
| Proliferation | Modified MS | BAP (0.00, 2.22, 4.44, 8.88 μM) or KIN (0.00, 2.32, 4.65, 9.29 μM) or TDZ (0.00, 0.45, 2.27, 4.54 μM) with GA3 (0.87 μM) and IAA (0.06 μM) | 3% sucrose | 0.7% agar |
| Rooting | Modified MS | – | 3% sucrose | 0.7% agar |