Diversity analysis and cross-species amplification of custard apple (Annona squamosa) using simple sequence repeats developed via next-generation sequencing technology

Motif types	Number of SSRs	Frequency (%)
Mono-nucleotide	100658	56.20
Di-nucleotide	46493	25.96
Tri-nucleotide	15590	8.70
Tetra-nucleotide	2266	1.26
Penta-nucleotide	495	0.27
Hexa-nucleotide	202	0.11
Compound nucleotide	13376	7.4
Total	179080

1 SSR, simple sequence repeat.

Table 2.

Genetic analysis of SSR primers data using. A. squamosa genotypes.

Locus	Forward sequence and reverse sequence 5′–3′	Repeat motif*	Tm (°C)	No. of alleles per locus (k)	Ho	He	PIC	PI
ASIIHR01 F ASIIHR01 R	ACATGCCCTCAATCATCTCC AGTTAAAAATTAATGAATGAGCCAGG	(TA)₆	55	23	0.50	0.93	0.91	0.0103
ASIIHR02 F ASIIHR02 R	CTGAATTCTAACAGATGTGCTGGG GCTTTGGACGTCTACGCAT	(TA)₈	60	20	0.60	0.91	0.90	0.0150
ASIIHR03 F ASIIHR03 R	TCAGACTTCAACTCAAGTCGATCC TAATACCCAAGTCATGAAGACCAA	(AT)₈	55	25	0.62	0.93	0.91	0.0114
ASIIHR04 F ASIIHR04 R	ACTCGTACTGCTATAAAAGTGGGT ATGAGAGCTGCCCACGAC	(AT)₇	55	37	0.65	0.97	0.95	0.0032
ASIIHR05 F ASIIHR05 R	AAAACTGTCGGCTTCCATGT AGA AC C A AC AC AGC TTC C ATT	(TA)₆	55	20	0.80	0.84	0.82	0.0364
ASIIHR06 F ASIIHR06 R	CTTCTGTTGTCATCACTCGCA CACGATAGAAATCGAAGAACAGA	(TA)₆	55	22	0.52	0.90	0.88	0.0186
ASIIHR07 F ASIIHR07 R	AAATATCAAGTTTAAAGCAGTATTTGC TACAAGCGGGACATAGAAGC	(TA)₆	55	30	0.97	0.95	0.93	0.0064
ASIIHR08 F ASIIHR08 R	GATAGGAAGACAAACAGTTAGTTTAGG CGGCAGCTTTCTTCTTGTTC	(AG)₆	55	28	0.92	0.91	0.90	0.0143
ASIIHR09 F ASIIHR09 R	CCAAGAAATCTCACGTTCGC TCTTTCTTTAGCAAGTAGTGGTTTTT	(AG)₁₃	60	30	0.82	0.95	0.93	0.0065
ASIIHR10 F ASIIHR10 R	TGTGGGTTTATATTGACCATCATT GTGGAGCTAGGGTTCTTCGTC	(GA)₈	60	31	0.97	0.93	0.94	0.0057
ASIIHR11 F ASIIHR11 R	ACGCTTTTCTTCTCCGGC CTCGCCTGCTCCTCTCAC	(GA)₉	55	26	0.52	0.95	0.91	0.0110
ASIIHR12 F ASIIHR12 R	GCTTTGAGAGAAAATGAGAGACAA CTACCTTTCCGGCGAATCC	(AG)₁₁	55	30	0.92	0.91	0.93	0.0065
ASIIHR13 F ASIIHR13 R	GATATTCAAAGAGCACGAGAGGA AAAAGTTAGTCGGCAAATCCC	(GA)₆	55	33	0.70	0.88	0.89	0.0147
ASIIHR14 F ASIIHR14 R	GTGAGAGAGAGAGAAGGAAGGC GCGAATCTCTCTTCCGACAG	(GA)₁₀	55	19	0.70	0.93	0.85	0.0284
ASIIHR15 F ASIIHR15 R	TTTTCTCTTTTCTTCGTTCTTGC GAGGGGGCTGGTGACCTAT	(GA)₁₂	60	25	0.82	0.92	0.91	0.0107
ASIIHR16 F ASIIHR16 R	CCTAATCGGAAAGGTGCAAA TGGCTTTATTGGATGTGTTTGA	(AC)⁷	55	23	0.70	0.85	0.91	0.0126
ASIIHR17 F ASIIHR17 R	GCTAAGACGGGGCCAACC TGCCTTATTTCTTTAAACAGGGTC	(AC)₆	60	16	0.75	0.87	0.83	0.0388
ASIIHR18 F ASIIHR18 R	CTCTCTCTTGTGCTTCTCCCA CTTTCTCTCTCTCCCCCTCC	(AC)₆	55	18	0.82	0.97	0.84	0.0338
ASIIHR19 F ASIIHR19 R	TGACGAGATCGAATTAAGTACCC TGATGCCTATAAAAGGGGCA	(CA)₈	60	46	0.92	0.94	0.96	0.0022
ASIIHR21 F ASIIHR21 R	ACCAGCAAATCCTGGGAAG TGAATCTGCAACTCAAAACTGA	(AC)₆	55	29	1.00	0.78	0.93	0.0076
ASIIHR21 F ASIIHR21 R	TTGATGCAATTCTTCAGTTTGA TACAAGGGTTGGGAATTTGG	(AC)₈	55	16	0.92	0.92	0.74	0.0758
ASIIHR22 F ASIIHR22 R	CATACATTTTGCCCACGACC CACAGATACACACACACGAACAA	(GT)₈	60	25	0.77	0.95	0.90	0.0132
ASIIHR23 F ASIIHR23 R	AAAAAGTCCATTCTTTTTCTCCA TCATTTCTTCATCCCATTGC	(GT)₆	55	28	0.92	0.94	0.93	0.0066
ASIIHR24 F ASIIHR24 R	CACATCACCCATATAAAAAGCG GTTGGGACAACTCTTCACCC	(TG)₆	60	35	0.82	0.93	0.93	0.0065
ASIIHR25 F ASIIHR25 R	CAGCGATGGTTGCTTAATTTG AGTAGGTGGAGAGACCCACG	(GT)₆	55	25	0.77	0.93	0.92	0.0099
ASIIHR26 F ASIIHR26 R	AGCAAAAGTGGTCATCCGAA TTTTAGATCGCTAAGAAGATCACA	(TG)₁₃	60	30	0.80	0.96	0.91	0.0102
ASIIHR27 F ASIIHR27 R	TCGCTATTTCAAAATTAAGTAAAAGAA TGTTTTAGTTGAGCAAGGCTAGG	(TG)₈	55	37	0.82	0.96	0.95	0.0044
ASIIHR28 F ASIIHR28 R	TCTTGTTTTTGCCAGTTCCC GCTTGAACTCAAAGCATGTTG	(GT)₈	60	34	0.87	0.87	0.95	0.0038
ASIIHR29 F ASIIHR29 R	TGTTACTGTTGGGCATGGAA GGTTGGGTTGAAAATTTAAGCA	(GT)₆	55	23	0.67	0.95	0.85	0.0263
ASIIHR30 F ASIIHR30 R	CCTTCCACCCTTGGATCTTA GATCAATGTGGATAAAACTTCGC	(CT)₆	55	33	0.82	0.97	0.94	0.0058
ASIIHR31 F ASIIHR31 R	CTTTTCTTCTCCATTTTCCCG CGTTGGAGATTCCAAGAGAAA	(CT)₈	55	44	0.95	0.95	0.95	0.0031
ASIIHR32 F ASIIHR32 R	AGGTGGATCGCTTAAGATGAA TCTGAGCAGGTGTGTAGTCCT	(TC)₆	55	29	0.80	0.92	0.93	0.0069
ASIIHR33 F ASIIHR33 R	ACTGGCCGAGGAAAGGGT GAGAGGGAGGAAAAAGTTAATCG	(TC)₁₂	55	28	0.97	0.97	0.91	0.0117
ASIIHR34 F ASIIHR34 R	CTCCCCGTTACCCAACTG GGTTCCTTCCGTCCTCCTG	(CT)₉	55	35	0.72	0.96	0.95	0.0034
ASIIHR35 F ASIIHR35 R	TTTC ATAGC TTTTATTGC TTTCTTAG AGTCGGGTCGAATCCACA	(GA)₈	55	40	0.85	0.96	0.95	0.0036
ASIIHR36 F ASIIHR36 R	CTTCTGTCTTCCTCATTTTCTCG TGGGAGAGAAGTGAGAGAGC TT	(AG)₈	60	35	0.77	0.94	0.94	0.0047
ASIIHR37 F ASIIHR37 R	GGCCACACTTGCTCAAAAAT TTCTTATTGATGCGTAGCGG	(GC)₇	55	19	0.92	0.90	0.92	0.0090
ASIIHR38 F ASIIHR38 R	GGGAGGAAACTTGATCCCTT AAAATTATGGTGCAGTGGCG	(GC)₆	60	15	0.27	0.94	0.88	0.0196
ASIIHR39 F ASIIHR39 R	GGCCACACTTGCTCAAAAAT TTCTTATTGATGCGTAGCGG	(GC)₇	55	28	0.27	0.97	0.92	0.0087
ASIIHR40 F ASIIHR40 R	CCAATCCCTTTATCCAAGCA GTGCAATTTGAAAAGCAGCA	(GC)₇	55	35	0.27	0.93	0.96	0.0027
ASIIHR41 F ASIIHR41 R	CATCTCCGCAACACCAGATA GCCAGAAGAGGCAGTGATTC	(GC)₈	55	27	0.67	0.94	0.92	0.0092
ASIIHR42 F ASIIHR42 R	AGAGGAAAACTTACAAAAACATAGACG GCCCTAAGAGAGCAGTTTACCC	(ATG)₆	55	26	0.30	0.93	0.92	0.0089
ASIIHR43 F ASIIHR43 R	GTATGTCATGGAGGATACAGGGA CATCCTCATCATCTCCCACA	(GAA)₅	55	23	0.25	0.90	0.92	0.0091
ASIIHR44 F ASIIHR44 R	ACTGCTGCTGAGATGTGCG CTGCTGCTGCTGTTGACG	(GAT)₇	55	23	0.30	0.92	0.91	0.0112
ASIIHR45 F ASIIHR45 R	TTATTGTATAAAACACCCCAAAGAA TTCTTATCATTTTGCCCGTCT	(TTC)₁₀	60	18	0.45	0.96	0.89	0.0183
ASIIHR46 F ASIIHR46 R	TTGGCAACCATCAGAATAAGA ACAACCCTGCTTCCATTCAA	(AAT)₅	60	17	0.35	0.94	0.90	0.0152
ASIIHR47 F ASIIHR47 R	AAAAACCTTGGGCTTGTGC CCTTGCCCCTATTATTTTCC	(TCT)₆	55	32	0.37	0.95	0.95	0.0037
ASIIHR48 F ASIIHR48 R	TTGGTGAAGCATTCAAAAATTC CCTTGCCCCTATTATTTTCC	(ATG)₁₀	55	32	0.52	0.93	0.93	0.0072
ASIIHR49 F ASIIHR49 R	TCAAACGCCCGCATATTTA GCTGGAGAAAGACGGCAAG	(AGC)₅	55	26	0.40	0.94	0.93	0.0070
ASIIHR50 F ASIIHR50 R	ACCTCAAAGCTAGGGGGTAAA CCGAAGTAGAGATACGCCTCTT	(GAA)₅	55	26	0.35	0.93	0.92	0.0103
ASIIHR51 F ASIIHR51 R	AAAATGAGCATGAAGAAAAGAAAAA GATTGTAAGACAAATTGAGATGTAATG	(ATT)₁₀	55	22	0.30	0.94	0.93	0.0081
ASIIHR52 F ASIIHR52 R	TCCCATTTTCTGATCGAGTTG TAACCCTCGCCGTGAATAG	(TCA)₅	55	23	0.42	0.93	0.92	0.0101
ASIIHR53 F ASIIHR53 R	AGACTAATCTAAGTTTAAAGCAAGCAA TGATC TTTGTTGAAGCGTCTCT	(AAG)₅	55	21	0.45	0.94	0.92	0.0090
ASIIHR54 F ASIIHR54 R	TTCAAGAATCATCTTTTAAGTCAACC TTTTCATGGAAACAACCAAATG	(GTG)₅	55	30	0.45	0.94	0.92	0.0079
ASIIHR55 F ASIIHR55 R	TCACTTGGAATAATGTGGAACG CAGTCCCCAGCTCCAAAC	(GTC)₁₁	55	19	0.40	0.91	0.89	0.0181
ASIIHR56 F ASIIHR56 R	CCCCAATCCCAATCCTTAGT GGAGTTCGTGTGCTTTACCG	(AGC)₅	55	28	0.75	0.96	0.94	0.0047
ASIIHR57 F ASIIHR57 R	GACGTGCTGCTG GGATTCTTCACCAGGCAGTT	(CGA)₅	55	24	0.35	0.92	0.90	0.0134
ASIIHR58 F ASIIHR58 R	AAAATGCATGCCTTGTTTGT TAGTCCTTGAGCAACACATGC	(ATTC)₇	60	23	0.55	0.91	0.90	0.0146
ASIIHR59 F ASIIHR59 R	AAGGGCATGTTGTCTTCTCAA TTTGCAAGTTTATTTCTGCTCAA	(TTGT)₆	60	29	0.45	0.95	0.93	0.0070
ASIIHR60 F ASIIHR60 R	TCCCGACCTTTCTTACGGAT TTTATCTCTATCTCTCCACCGGA	(ATCTC)₆	60	22	0.67	0.87	0.85	0.0276
ASIIHR61 F ASIIHR61 R	AATATGTTAACCCGAAACTCAACC AATAATTACATAATTGATGAGGGTCAA	(TAGGGT)₅	55	16	0.60	0.95	0.78	0.0571
ASIIHR62 F ASIIHR62 R	CTCTGTTTCTATCTCTCTCAAACTCA GGAATGGGAGAGTATTTGAAGG	(TC)₉tgtctctcatt(TC)₆	55	34	0.50	0.87	0.94	0.0058
ASIIHR63 F ASIIHR63 R	TACCGGATCTCTCATTTTCG GCTGGGAGAGTGAGCTGAAA	(TC)₅cgccatttctatctct ctctccgtcattcct ctctctctttctctgttttccttt ggaaaaatcggca aacccaaat(TC)₆	55	24	0.62	0.81	0.90	0.0139
ASIIHR64 F ASIIHR64 R	CATGTTGGACATGTGAGCCA ATGCCTATTTTAGGCTGGGT	(A)₁₀g(A)₁₀	55	28	0.85	0.95	0.91	0.0101
ASIIHR65 F ASIIHR65 R	GGCGTCCAAAAATTGAGATT TTTGGGGAGTATCTACTCAGGC	(AT)₇gtatttgccccatggg ccccaaaaaaaataaa(AT)₇ ttttagactttcaa(AT)₇	55	31	0.67	0.92	0.92	0.0082
ASIIHR66 F ASIIHR66 R	TCTACGCTACCCAGCAAATG AATGACAATATGATTTGCCTTGA	(T)₁₁(TA)₆*	55	28	0.67	0.93	0.90	0.0128
ASIIHR67 F ASIIHR67 R	CCGACTTCAACCTTCTGAGC TCAATGGAATATTCACTTTTCTAGG	(A)₁₁ttaaaacagattta tcaaaaatgttctta cgagaaagggaaaa taggagaaaaaggt agaatgagggttttc tttttgtgcgtgttttgga(AG)₈	55	27	0.60	0.94	0.91	0.0075
ASIIHR68 F ASIIHR68 R	GTGGATACTCCCCGACTGG TTCACATACTTTTGCCTGAGTAGA	(AAT)₆	55	26	0.55	0.95	0.92	0.0066
ASIIHR69 F ASIIHR69R	TTCACATACTTTTGCCTGAGTAGA GCCATCTTGGGCTTTTTAGA	(AT)₆gacctcttcgctcaatccaagcctcaatgtc(A)₁₀	55	21	0.67	0.92	0.90	0.0143
ASIIHR70F ASIIHR70 R	GAAGGGAGATGCAAACGTTAAG AACTGCTTGCTTTATGCACTTT	(A)₁₁(T)₁₀	55	27	0.60	0.92	0.91	0.0118

* Indicates the number of times a particular motif was repeated in the microsatellite locus. For example: (TA₆ – means TA has been repeated six times in the sequence results analysed during SSR identification. He, expected heterozygosity; Ho, observed heterozygosity; PI, probability of identity; PIC, polymorphic information content; SSR, simple sequence repeat.

Dendrogram analysis of *A. squamosa* genotypes using SSR markers data. SSR, simple sequence repeat.

Structure analysis of *Annona* genotypes using SSR data.

Table 3.

Cross species amplification of 70 SSR loci derived from A. squamosa.

No.	Locus	A. cherimola	A. reticulata	A. glabra	A. muricata	A. atemoya
1	ASIIHR01	A	A	A	A	A
2	ASIIHR02	A	A	A	A	A
3	ASIIHR03	A	A	A	A	A
4	ASIIHR04	A	A	A	A	A
5	ASIIHR05	A	A	A	A	A
6	ASIIHR06	A	A	A	A	A
7	ASIIHR07	NA	A	NA	A	NA
8	ASIIHR08	A	A	A	A	A
9	ASIIHR09	A	A	A	A	A
10	ASIIHR10	A	A	A	A	A
11	ASIIHR11	A	A	A	A	A
12	ASIIHR12	A	A	A	A	A
13	ASIIHR13	A	A	A	A	A
14	ASIIHR14	A	A	A	A	A
15	ASIIHR15	A	A	A	A	A
16	ASIIHR16	A	A	A	A	A
17	ASIIHR17	A	A	A	A	A
18	ASIIHR18	A	A	A	A	A
19	ASIIHR19	A	A	A	A	A
20	ASIIHR20	A	A	A	A	A
21	ASIIHR21	A	A	A	A	A
22	ASIIHR22	A	A	A	NA	NA
23	ASIIHR23	A	A	A	A	A
24	ASIIHR24	A	A	A	A	A
25	ASIIHR25	A	A	A	A	A
26	ASIIHR26	A	A	A	A	A
27	ASIIHR27	NA	A	A	A	NA
28	ASIIHR28	A	A	A	A	A
29	ASIIHR29	A	A	A	A	A
30	ASIIHR30	A	A	A	A	A
31	ASIIHR31	A	A	A	A	A
32	ASIIHR32	A	A	A	A	A
33	ASIIHR33	A	A	A	A	A
34	ASIIHR34	A	A	A	A	A
35	ASIIHR35	A	A	A	A	NA
36	ASIIHR36	A	A	A	A	A
37	ASIIHR37	A	A	A	A	A
38	ASIIHR38	A	A	A	A	A
39	ASIIHR39	A	A	A	A	A
40	ASIIHR40	A	A	A	A	A
41	ASIIHR41	A	A	NA	A	NA
42	ASIIHR42	A	A	A	A	A
43	ASIIHR43	A	A	A	A	A
44	ASIIHR44	A	A	A	A	A
45	ASIIHR45	A	A	A	A	A
46	ASIIHR46	A	A	A	A	A
47	ASIIHR47	A	A	A	A	A
48	ASIIHR48	A	A	A	A	NA
49	ASIIHR49	A	A	A	A	A
50	ASIIHR50	A	A	A	A	A
51	ASIIHR51	NA	A	A	A	A
52	ASIIHR52	A	A	A	NA	NA
53	ASIIHR53	NA	A	A	A	NA
54	ASIIHR54	A	A	A	A	A
55	ASIIHR55	A	A	A	A	A
56	ASIIHR56	A	A	A	A	A
57	ASIIHR57	A	A	A	A	A
58	ASIIHR58	A	NA	A	NA	NA
59	ASIIHR59	A	A	A	A	A
60	ASIIHR60	A	A	A	NA	NA
61	ASIIHR61	A	A	A	A	A
62	ASIIHR62	A	NA	A	NA	NA
63	ASIIHR63	A	A	A	A	A
64	ASIIHR64	A	A	A	A	A
65	ASIIHR65	A	A	A	NA	NA
66	ASIIHR66	A	A	A	A	A
67	ASIIHR67	A	A	A	A	A
68	ASIIHR68	A	NA	A	NA	NA
69	ASIIHR69	A	A	A	A	A
70	ASIIHR70	A	A	A	A	A
Transferability %		94.2	95.7	97.1	90.00	80.00

1 A, amplified, NA, not amplified; SSR, simple sequence repeat.

Table 4.

AMOVA analysis of genetic variances within and among populations of 40 custard apple accessions.

Source of variation	df	SS	MS	Estimated variance	% Variation
Among populations	5	213.769	42.74	0.021	0
Among individuals	34	1447.244	42.56	9.870	30
Within individuals	40	913.00	22.825	22.825	70
Total					100

1 AMOVA, analysis of molecular variance; df, degrees of freedom; MS, mean sum of squares; SS, sum of squares.

Supplementary Table 1.

List of 40 custard apple (A. squamosa) genotypes/cultivars belonging to A. squamosa and different species of Annona used in the study.

Sl. No.	Cultivars/accessions No.	Place of collection	State
1.	Balanagar	Sangareddy	Telangana
2.	Raidurg	Ananthapur	Andhra Pradesh
3.	Taiwan	Taiwan	Taiwan
4.	Arka_Sahan (IC No. 0632061)	IIHR	Karnataka
5.	NMK_1	Garmale	Maharashtra
6.	APK_1	Aruppukottai	Tamil Nadu
7.	WASHINGTON_05	Florida	USA
8.	Barbados	Florida	USA
9.	Washington_97	Florida	USA
10.	19/26 (IC no. 0632061)	IIHR, Bangalore	Karnataka
11.	Arka Neelanchal Vikram	Bhubaneswar	Odisha
12.	Mammoth	Florida	USA
13.	Red_sitaphal	Sangareddy	Telangana
14.	6_8	Nalakadoddi	Andhra Pradesh
15.	2_1	Vengalampalli	Andhra Pradesh
16.	3_1	Vengalampalli	Andhra Pradesh
17.	1_1	Vengalampalli	Andhra Pradesh
18.	8_8	Pythota	Andhra Pradesh
19.	8_9	Pythota	Andhra Pradesh
20.	2_2	Vengalampalli	Andhra Pradesh
21.	27_1	Jambugumpala	Andhra Pradesh
22.	5_8	Molakalmuru	Karnataka
23.	3_2	Vengalampalli	Andhra Pradesh
24.	5_1	Molakalmuru	Karnataka
25.	8_18	Pythota	Andhra Pradesh
26.	1_10	Vengalampalli	Andhra Pradesh
27.	8_16	Pythota	Andhra Pradesh
28.	11_8	Yercaud	Tamil Nadu
29.	3_3	Vengalampalli	Andhra Pradesh
30.	4_11	Molakalmuru	Karnataka
31.	8_17	Pythota	Andhra Pradesh
32.	1_5	Vengalampalli	Andhra Pradesh
33.	2_13	Vengalampalli	Andhra Pradesh
34.	4_1	Molakalmuru	Karnataka
35.	2_10	Vengalampalli	Andhra Pradesh
36.	4_10	Molakalmuru	Karnataka
37.	6_10	Nalakadoddi	Andhra Pradesh
38.	4_12	Molakalmuru	Karnataka
39.	8_7	Pythota	Andhra Pradesh
40.	Rolenia	Moodubidire	Karnataka
41.	A. cherimola	Sangareddy	Telangana
42.	A. reticulata	Sangareddy	Telangana
43.	A. glabra	Sangareddy	Telangana
44.	A. muricata	Sangareddy	Telangana
45.	A. atemoya	Sangareddy	Telangana

Supplementary Table 2.

Summary of sample Balanagar assembled genome.

Plant WGS	Balanagar
Contigs generated	1388525
Maximum contig length	21462
Minimum contig length	100
Average contig length	466.126
Total contigs length	647227553 (647.2 MB)
Total number of non-ATGC characters	54429
Percentage of non-ATGC characters	8.40956E-05
Contigs >100 b:	1379816
Contigs >500 b:	350135
Contigs >1 Kb:	170254
Contigs >10 Kb:	19
Contigs >100 Kb:	0
n50 value:	954
n90_value:	151
kmer length	89
Total number of scaffolds	58528
Total number of identified SSRs	1790080

1 SSR, simple sequence repeat.

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DOI: https://doi.org/10.2478/fhort-2025-0009 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761

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Language: English

Page range: 139 - 155

Submitted on: Feb 6, 2025

Accepted on: May 20, 2025

Published on: Jul 10, 2025

Published by: Polish Society for Horticultural Sciences (PSHS)

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

Annona squamosa species,

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© 2025 Abhilasha Krishnamurthy, Thandavarayan Sakthivel, Kundapura V. Ravishankar, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 37 (2025): Issue 1 (July 2025)

Diversity analysis and cross-species amplification of custard apple (Annona squamosa) using simple sequence repeats developed via next-generation sequencing technology

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Table 1.

Table 2.

Figure 1.

Figure 2.

Table 3.

Table 4.

Supplementary Table 1.

Supplementary Table 2.

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