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The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (Fragaria vesca L.) in vitro Cover

The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (Fragaria vesca L.) in vitro

Folia Horticulturae
Volume 35 (2023): Issue 2 (December 2023)
By:
Open Access
|Dec 2023

Figures & Tables

Figure 1.

Gene expression in wild strawberry microshoots grown on MS medium with different concentrations of sucrose. MS medium with 3% sucrose was used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; MS, Murashige and Skoog; Myb10, MYB domain protein 10; UFGT, UDP glucose: flavonol 3-O-glucosyltransferase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown on MS medium with different concentrations of sucrose. MS medium with 3% sucrose was used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; MS, Murashige and Skoog; Myb10, MYB domain protein 10; UFGT, UDP glucose: flavonol 3-O-glucosyltransferase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Figure 2.

Gene expression in wild strawberry microshoots grown on MS medium with different concentrations of PEG. MS medium with 3% sucrose was used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHS, chalcone synthase; DFR, dihydroflavonol reductase; EGL, enhancer of glabra; MS, Murashige and Skoog; PEG, polyethylene glycol; UFGT, UDP glucose: flavonol 3-O-glucosyltransferase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown on MS medium with different concentrations of PEG. MS medium with 3% sucrose was used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHS, chalcone synthase; DFR, dihydroflavonol reductase; EGL, enhancer of glabra; MS, Murashige and Skoog; PEG, polyethylene glycol; UFGT, UDP glucose: flavonol 3-O-glucosyltransferase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Figure 3.

Wild strawberry microshoots grown under different light spectra: fluorescent light (F), LED lighting system: blue light (B), red light (R), blue + red lights (BR), and blue + red + UV lights (BR UV). LED, light-emitting diode.
Wild strawberry microshoots grown under different light spectra: fluorescent light (F), LED lighting system: blue light (B), red light (R), blue + red lights (BR), and blue + red + UV lights (BR UV). LED, light-emitting diode.

Figure 4.

Gene expression in wild strawberry microshoots grown at 15°C for 1-4 weeks. Microshoots grown at 22°C were used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown at 15°C for 1-4 weeks. Microshoots grown at 22°C were used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Figure 5.

Gene expression in wild strawberry microshoots grown at 30°C for 1-4weeks. Microshoots grown at 22°C were used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol reductase; F3H, flavanone 3-hydroxylase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown at 30°C for 1-4weeks. Microshoots grown at 22°C were used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol reductase; F3H, flavanone 3-hydroxylase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Supplementary Figure S1.

Gene expression in wild strawberry microshoots grown on MS medium under different light spectra. Microshoots grown under fluorescent lamps were used as a control. Bars denote the standard error of the mean. CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol reductase; F3H, flavanone 3-hydroxylase; MS, Murashige and Skoog; Myb10, MYB domain protein 10; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown on MS medium under different light spectra. Microshoots grown under fluorescent lamps were used as a control. Bars denote the standard error of the mean. CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol reductase; F3H, flavanone 3-hydroxylase; MS, Murashige and Skoog; Myb10, MYB domain protein 10; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Primers used for anthocyanin biosynthesis gene expression analysis

GeneAccession numberPrimer sequenceAnnealing temperature, °CProduct size, bp
Myb10EU155163F: 5’-CGGAAGATTGCCAGGAAGAAC-3’62.4165
R: 5’-ATGAAGGTTCGTGGTCGAGG-3’63.1
WD40-TTG1XM_004307863F: 5’-AGCAGGACTTGAGGTACATGG-3’63129
R: 5’-ACGCAATCGCATTCACACTC-3’62.8
EGLXM_004308329F: 5’-GCCTTCGATAAACAAGCGGAAG-3’63.1131
R: 5’-TCTCTATCAGAACCTCCTGCTC-3’61.9
UFGTXM_004307828F: 5’-GCGCATGGTTCAGTTGGAG-3’62.8151
R: 5’-GACCAATCTTCCACACATCCTC-3’62
DFRKC894052F: 5’-GTCTCATTACCGGACTTTCGC-3’62.1131
R: 5’-CTCTGCTTTCGGATGCTCG-3’61.9
F3HAB201760F: 5’-CACAGCAGGTTGTCCATAGC-3’62.2114
R: 5’-AGTGTAAGTCATCGGCTCCTC-3’62.6
CHIXM_0043074035’-AAAGATCAGACCTTCCCACCC-3’63119
R: 5’-TCAATCACCGCATTCCCAAC-3’62.4
CHSAB250913F: 5’-ACTTTTCTGGATTGCACACCC-3’62.6189
R: 5’-GTCTTGTGCCCATTAGCTGC-3’62.8
β-actin*XM_004306544F: 5’-TCAACTATGTTCCCTGGTATTGC-3’62175
R: 5’-CTCCCTTGGAAATCCACATCTG-3’62.1

Average weight of wild strawberry microshoots, affected by osmotic components in MS medium, light and growth temperature_

TraitParameterAverage weight of microshoot (g)
Osmotic componentsSucrose 15 g ∙ L-10.27 ± 0.03
Sucrose 30 g ∙ L-10.30 ± 0.03
Sucrose 60 g ∙ L-10.13 ± 0.04*
Sucrose 90 g ∙ L-10.10 ± 0.04*
Sucrose 30 g ∙ L-1 + PEG 50 g ∙ L-10.12 ± 0.10*
Sucrose 30 g ∙ L-1 + PEG 100 g ∙ L-10.11 ± 0.11*
Sucrose 30 g ∙ L-1 + PEG 120 g ∙ L-10.44 ± 0.60*
LightFluorescent0.28 ± 0.01
Blue0.31 ± 0.02
Red0.27 ± 0.02
BR0.34 ± 0.03
BRUV0.24 ± 0.01
Temperature15°C0.13 ± 0.03*
22°C0.34 ± 0.06
30°C0.30 ± 0.15
DOI: https://doi.org/10.2478/fhort-2023-0030 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
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Language: English
Page range: 419 - 431
Submitted on: Oct 26, 2022
Accepted on: Oct 24, 2023
Published on: Dec 31, 2023
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 times per year
Keywords:
controlled conditions,
flavonoids,
growth,
metabolism,
pigmentation,
stress
Related subjects:
Life sciences,
Plant science,
Zoology,
Ecology,
Life sciences, other

© 2023 Jurgita Vinskienė, Vidmantas Bendokas, Vidmantas Stanys, Audrius Sasnauskas, Rytis Rugienius, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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