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Salicylic acid effect on the mechanism of Lelliottia amnigena causing potato soft rot Cover

Salicylic acid effect on the mechanism of Lelliottia amnigena causing potato soft rot

Folia Horticulturae
Volume 33 (2021): Issue 2 (December 2021)
By: Richard Osei,  Chengde Yang,  Lingxiao Cui,  Lijuan Wei,  Mengjun Jin and  Solomon Boamah  
Open Access
|Dec 2021

Figures & Tables

Figure 1

Effect of SA on (A) pectinase, (B) protease, (C) pectin lyase and (D) cellulase production of L. amnigena (Strain PC3) stress. Data are presented as mean ± SE. Different letters indicate a significant difference according to Duncan's multiple range test (p < 0.05). SA, salicylic acid.
Effect of SA on (A) pectinase, (B) protease, (C) pectin lyase and (D) cellulase production of L. amnigena (Strain PC3) stress. Data are presented as mean ± SE. Different letters indicate a significant difference according to Duncan's multiple range test (p < 0.05). SA, salicylic acid.

Figure 2

Symptoms of artificial infection with strain PC3 14 DAI. Tubers were treated with SA 24 h before inoculation with strain PC3. (A) Control (CK), (B) strain PC3 (C) 0.5 + PC3, (D) 1.0 + PC3, (E) 1.5 + PC3, (F) 2.0 + PC3. (G) DI (H) Disease reduction. Data are presented as mean ± SE. Different letters indicate a significant difference according to Duncan's multiple range test (p < 0.05). Potato tubers were treated with different concentrations of SA for 24 h before inoculation with strain PC3. The treatments were water (negative control), strain PC3 (positive control), PC3 + 0.5 mM SA, PC3 + 1.0 mM SA, PC3 + 1.5 mM SA, PC3 + 2.0 mM SA. Strain PC3: L. amnigena, DAI. Figure 2A, tuber treated with distilled water showed no signs of rot, Figure 2B tuber inoculated with L. amnigena without SA treatment showed the highest signs of rot. Figure 2C–2F, tubers inoculated with L. amnigena and treated with different concentrations of SA showed minor signs of rot with Figure 2F showing very little sign of rot. DAI, days after inoculation; DI, disease index; SA, salicylic acid.
Symptoms of artificial infection with strain PC3 14 DAI. Tubers were treated with SA 24 h before inoculation with strain PC3. (A) Control (CK), (B) strain PC3 (C) 0.5 + PC3, (D) 1.0 + PC3, (E) 1.5 + PC3, (F) 2.0 + PC3. (G) DI (H) Disease reduction. Data are presented as mean ± SE. Different letters indicate a significant difference according to Duncan's multiple range test (p < 0.05). Potato tubers were treated with different concentrations of SA for 24 h before inoculation with strain PC3. The treatments were water (negative control), strain PC3 (positive control), PC3 + 0.5 mM SA, PC3 + 1.0 mM SA, PC3 + 1.5 mM SA, PC3 + 2.0 mM SA. Strain PC3: L. amnigena, DAI. Figure 2A, tuber treated with distilled water showed no signs of rot, Figure 2B tuber inoculated with L. amnigena without SA treatment showed the highest signs of rot. Figure 2C–2F, tubers inoculated with L. amnigena and treated with different concentrations of SA showed minor signs of rot with Figure 2F showing very little sign of rot. DAI, days after inoculation; DI, disease index; SA, salicylic acid.

Figure 3

Effect of SA on (A) Plant height, (cm) (B) number of leaves, (C) leaf area (cm2), (D) fresh weight (g) and (E) dry weight (g) under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by least significant difference (LSD) test (p < 0.05). SA, salicylic acid.
Effect of SA on (A) Plant height, (cm) (B) number of leaves, (C) leaf area (cm2), (D) fresh weight (g) and (E) dry weight (g) under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by least significant difference (LSD) test (p < 0.05). SA, salicylic acid.

Figure 4

Effect of SA on (A) chlorophyll a (B) chlorophyll b, (C) total chlorophyll a + b, (D) carotenoid under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by LSD test (p < 0.05). SA, salicylic acid.
Effect of SA on (A) chlorophyll a (B) chlorophyll b, (C) total chlorophyll a + b, (D) carotenoid under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by LSD test (p < 0.05). SA, salicylic acid.

Figure 5

Effect of SA on (A) MDA content, (B) H2O2 content of potato leaves under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by LSD test (p < 0.05). H2O2, hydrogen peroxide; MDA, malondialdehyde; SA, salicylic acid.
Effect of SA on (A) MDA content, (B) H2O2 content of potato leaves under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by LSD test (p < 0.05). H2O2, hydrogen peroxide; MDA, malondialdehyde; SA, salicylic acid.

Figure 6

Effect SA on (A) SOD activity, (B) POD activity, (C) CAT activity and (D) PPO activity of potato leaves under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by LSD test (p < 0.05). CAT, catalase; POD, peroxide; PPO, polyphenol oxidase; SA, salicylic acid; SOD, superoxide dismutase.
Effect SA on (A) SOD activity, (B) POD activity, (C) CAT activity and (D) PPO activity of potato leaves under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by LSD test (p < 0.05). CAT, catalase; POD, peroxide; PPO, polyphenol oxidase; SA, salicylic acid; SOD, superoxide dismutase.

Figure 7

Effect of SA on expression patterns of SOD (A), POD (B), CAT (C), and GST (D) in leaves of potato plants under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by LSD test (p < 0.05). CAT, catalase; GST, glutathione S-transferase; POD, peroxide; SA, salicylic acid; SOD, superoxide dismutase.
Effect of SA on expression patterns of SOD (A), POD (B), CAT (C), and GST (D) in leaves of potato plants under L. amnigena (Strain PC3) stress, where CK represents control treatment with distilled water. Data represent mean ± SE of three replicates. Lower case letters indicate statistical significance between treatments by LSD test (p < 0.05). CAT, catalase; GST, glutathione S-transferase; POD, peroxide; SA, salicylic acid; SOD, superoxide dismutase.

Treatment combinations_

GroupNameTreatment
T1Negative controlDistilled water
T2Positive controlStrain PC3 (L. amnigena)
T3PC3 + 0.5 mM SAInoculation of strainPC3 + 0.5 mM SA
T4PC3 + 1.0 Mm SAInoculation of strainPC3 + 1.0 mM SA
T5PC3 + 1.5 Mm SAInoculation of strainPC3 + 1.5 mM SA
T6PC3 + 2.0 Mm SAInoculation of strainPC3 + 2.0 mM SA

Gene description and primers sequences used for the qRT-PCR_

Gene symbolDescriptionPrimer sequence (5′–3′)Experiment
SODManganese superoxide dismutaseF-GCTGGTGCTAGAGTAGCCTGR-TCAAAGTTATCTGCCGTTCTCCAAntioxidant activity test
PODPeroxidaseF-GTTGCCTTATCAGGTTTGTTCTTTR-CGGACGTGTTTGAATCAACTTAntioxidant activity test
CATCatalaseF-TTTCAGGAGATGTGCAGCGTR-ATGTATGAGGCTTTTATGCTGCTAntioxidant activity test
GSTGlutathione S-transferaseF-AGCTGGTGCCCATCAATCTCR-CCCTCTGAGACTCCTAATGATCCAntioxidant activity test
ACTActin-related protein ActinF-GCTCCTAGAGCTGTATTCCCAAGTR-CAGTCGAAACGTGGTATCTTGACTAntioxidant activity test

Pearson's correlation matrix among some parameters measured in the plant_

TraitsChlorophyllH2O2MDACarotenoid
Chlorophyll–
H2O2−0.948**–
MDA−0.985**0.975**–
Carotenoid0.987**−0.884*−0.951**–

Production of extracellular enzymes by suspensions (3_6 CFU · mL−1 × 107 CFU · mL−1) from L_ amnigena_

Extracellular enzymesEnzyme activity (U · mL−1)
Pectinase77.27 ± 3.441 b
Protease91.25 ± 4.672 a
Pectin lyase2.22 ± 0.102 c
Cellulase0.08 ± 0.009 c
DOI: https://doi.org/10.2478/fhort-2021-0029 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761
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Language: English
Page range: 376 - 389
Submitted on: Jul 29, 2021
Accepted on: Nov 29, 2021
Published on: Dec 30, 2021
Published by: Polish Society for Horticultural Sciences (PSHS)
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
antioxidant enzymes,
disease,
extracellular enzymes,
pathogen,
gene expression
Related subjects:
Life sciences,
Plant science,
Zoology,
Ecology,
Life sciences, other

© 2021 Richard Osei, Chengde Yang, Lingxiao Cui, Lijuan Wei, Mengjun Jin, Solomon Boamah, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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