Meiotic behaviour and pollen fertility of F1, F2 and BC1 progenies of Iris dichotoma and I. domestica

Wenji Xu; Gangjun Luo; Xiaoyin Lian; Fengyang Yu; Yang Zheng; Jiajun Lei; Xiaoying Bi

doi:10.2478/fhort-2021-0013

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Meiotic behaviour and pollen fertility of F1, F2 and BC1 progenies of Iris dichotoma and I. domestica

Folia Horticulturae

Volume 33 (2021): Issue 1 (June 2021)

By: Wenji Xu, Gangjun Luo, Xiaoyin Lian, Fengyang Yu, Yang Zheng, Jiajun Lei and Xiaoying Bi

Open Access

|May 2021

Abstract

Pollen characteristics are very important for Iris interspecific hybridisation. In this study, the pollen viability and male meiosis were studied in yellow-flowered Iris dichotoma (Y2), I. domestica (S3) and their hybrids F₁, F₂ and BC₁ (BC₁-Y and BC₁-S). The BC₁-Y hybrids showed higher pollen viability than that of F₁, F₂ and BC₁-S hybrids, which were between I. dichotoma (26.1%) and I. domestica (35.1%). Two sterile hybrids, F₂-1 and BC₁-S-1, exhibited more meiotic abnormalities (57.3% and 58.7%) than other individuals. During the first meiotic division, a diffuse diplotene stage was observed for the first time in the genus Iris. The meiotic abnormalities included non-congressed chromosomes, chromosome bridges, lagging chromosomes, unequal division, abnormally oriented spindle fibres, nonsynchronous division and polyad, and resulted in reduced pollen fertility. The relatively high frequency of 2n pollen grains was found in hybrids of BC₁-Y-2, BC₁-Y-1, BC₁-S-2, BC₁-S-3 and BC₁-S-4. Our research provides a new resource for meiotic behaviour and pollen fertility of the genus Iris.

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DOI: https://doi.org/10.2478/fhort-2021-0013 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761

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Language: English

Page range: 173 - 183

Submitted on: Feb 7, 2021

Accepted on: Apr 9, 2021

Published on: May 15, 2021

Published by: Polish Society for Horticultural Sciences (PSHS)

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

2n gametes,

abnormal meiosis,

interspecific hybrid,

male

Related subjects:

Life sciences,

Plant science,

Zoology,

Ecology,

Life sciences, other

© 2021 Wenji Xu, Gangjun Luo, Xiaoyin Lian, Fengyang Yu, Yang Zheng, Jiajun Lei, Xiaoying Bi, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 33 (2021): Issue 1 (June 2021)