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The influence of cultivation conditions on the adaptation of birch plants in vitro on the 14th and 30th days of cultivation
| Adaptation conditions | Basis of the substrate | Shoot height, cm | Root length, cm | ||
|---|---|---|---|---|---|
| Royal Frost | Jacquemontii | Royal Frost | Jacquemontii | ||
| 14th day of cultivation | |||||
| Wet chamber | peat | 8.8±0.60 | 7.3±0.55 | 3.2±0.41 | 3.0±0.30 |
| perlite | 9.7±0.72 | 7.8±0.62 | 3.6±0.49 | 3.0±0.42 | |
| Bioreactor | peat | 20.1±1.91 | 17.6±1.31 | 19.3±1.65 | 13.4±1.17 |
| perlite | 23.1±2.10 | 18.4±1.76 | 20.6±1.72 | 15.7±1.22 | |
| 30th day of cultivation | |||||
| Wet chamber | peat | 15.3±1.13 | 11.7±0.81 | 7.7±0.51 | 6.4±0.30 |
| perlite | 16.1±1.33 | 12.9±1.15 | 12.6±1.12 | 9.8±0.72 | |
| Bioreactor | peat | 39.6±2.31 | 32.1±2.32 | 29.7±2.33 | 23.3±1.93 |
| perlite | 45.4±3.48 | 33.7±2.64 | 33.0±2.84 | 27.6±2.44 | |
The influence of the age of donor plants on the features of decontamination and morphogenesis of primary birch explants in the MS medium
| Index | Variety | Age, years | ||
|---|---|---|---|---|
| 1 | 3 | 7 | ||
| Decontamination efficiency, % | royal frost | 31.4±4.09 | 23.2±3.11 | 14.1±2.07 |
| jacquemontii | 19.5±1.99 | 11.4±2.01 | 7.4±1.86 | |
| Morphogenesis of explants, % | royal frost | 11.9±2.02 | 9.0±1.98 | 6.1±2.02 |
| jacquemontii | 6.3±0.98 | 5.1±0.88 | 3.3±1.07 | |
Influence of growing conditions of annual donor plants on features of decontamination and morphogenesis of primary birch explants in the MS medium
| Index | Variety | Field conditions | Depository |
|---|---|---|---|
| Decontamination efficiency, % | royal frost | 30.4±4.01 | 47.7±5.13 |
| jacquemontii | 21.2±3.12 | 39.5±4.32 | |
| Morphogenesis of explants, % | royal frost | 10.2±2.08 | 23.3±3.22 |
| jacquemontii | 7.1±0.89 | 17.2±3.06 |
Dynamics of morphogenesis of birch explants on different media
| Index | Variety | Media | ||||
|---|---|---|---|---|---|---|
| MS | MS1/2 | |||||
| age, years | ||||||
| 1 | 5 | 1 | 5 | 7 | ||
| Decontamination efficiency, % | royal frost | 2.1±0.21 | 1.3±0.08 | 2.2±0.30 | 1.7±0.11 | 1.5±0.09 |
| jacquemontii | 1.6±0.10 | 1.1± 0.06 | 1.7±0.11 | 1.5±0.08 | 1.5±0.10 | |
| Morphogenesis of explants, % | royal frost | 39.5±3.09 | 12.1± 1.41 | 47±3.20 | 41.4±2.91 | 36.5±2.73 |
| jacquemontii | 33.2±2.82 | 11.0± 1.22 | 38±2.73 | 32.6±2.43 | 27.5±2.64 | |
The influence of combinations of nutrient media on shoot formation in birch regenerants during long-term cultivation
| Option | Index | |||
|---|---|---|---|---|
| height of a shoot, mm | number of nodes, pcs. | |||
| ...WPM–WPM... | ...MS1/2–WPM... | ...WPM–WPM... | ... MS1/2–WPM... | |
| Variety Royal Frost | 36.3±2.21 | 75.5±3.84 | 4.1±1.59 | 9.1±1.99 |
| Variety Jacquemontii | 29.4±1.98 | 65.2±3.32 | 3.3±1.42 | 7.4±2.43 |
Biometric indicators of birch regenerants on different nutrient media
| Index | Cutting | Medium | ||||
|---|---|---|---|---|---|---|
| MS | MS1/2 | DKW | QL | WPM | ||
| Royal Frost | ||||||
| The number of microshoots in the conglomerate, pcs | first | 2.0±0.21 | 2.2±0.17 | 1.9±0.14 | 1.8±0.21 | 3.9±0.10 |
| fifth | 1.3±0.18 | 1.7±0.31 | 1.8±0.30 | 1.8±0.33 | 4.1±0.22 | |
| Height of regenerants, mm | first | 36.2±3.3 | 47.4±4.2 | 44.4±5.12 | 32.0±0.29 | 63.8±4.1 |
| fifth | 31.1±2.90 | 41.1± 4.3 | 32.8±3.42 | 28.4±3.1 | 61.6±4.4 | |
| The number of internodes in the main shoot of regenerates, pcs | first | 2.4±0.21 | 2.9±0.11 | 2.7±0.40 | 2.3±0.22 | 3.6±0.10 |
| fifth | 1.7±0.18 | 2.3±0.16 | 1.3±0.31 | 2.1±0.19 | 3.5±0.08 | |
| Jacquemontii | ||||||
| The number of microshoots in the conglomerate, pcs | first | 1.4±0.22 | 1.7±0.19 | 2.0±0.11 | 2.2±0.21 | 3.7±0.29 |
| fifth | 1.1±0.28 | 1.5±0.30 | 1.6±0.21 | 1.7±0.27 | 3.6±0.20 | |
| Height of regenerants, mm | first | 32.3±3.1 | 38.6±5.5 | 41.2±3.3 | 30.4±3.1 | 59.9±3.6 |
| fifth | 21.2±2.4 | 32.6±4.8 | 27.7±2.4 | 25.3±2.2 | 62.8±3.4 | |
| The number of internodes in the main shoot of regenerates, pcs | first | 2.1±0.16 | 2.6±0.24 | 2.2±0.13 | 1.9±0.21 | 3.3±0.20 |
| fifth | 1.3±0.15 | 1.8±0.19 | 1.1±0.15 | 1.8±0.22 | 3.4±0.18 | |
The influence of combinations of nutrient media on the features of rhizogenesis of birch regenerants during long-term cultivation
| Option | Index | |||
|---|---|---|---|---|
| the length of the root system, mm | number of roots, pcs. | |||
| ...WPM–WPM... | ...MS1/2–WPM... | ...WPM–WPM... | ...MS1/2–WPM... | |
| Variety Royal Frost | 36±3 | 69±4 | 2±1 | 7±2 |
| Variety Jacquemontii | 11±3 | 37±3 | 5±2 | 14±2 |
Influence of the origin of primary bud explants on the features of decontamination and morphogenesis
| Index | Variety | Bud | ||
|---|---|---|---|---|
| apical | medial | basal | ||
| Decontamination efficiency, % | royal frost | 49.8±5.46 | 56.7±6.32 | 13.5±3.98 |
| jacquemontii | 38.3±4.05 | 48.5±5.23 | 9.1±2.86 | |
| Morphogenesis of explants, % | royal frost | 20.3±3.11 | 31.3±4.21 | 6.4±1.88 |
| jacquemontii | 16.6±3.16 | 27.6±3.09 | 3.21±2.01 | |
Decontamination and morphogenesis of primary birch explants with various antiseptics
| Index | Variety | NaClO | AgNO3 | C2H5OH | Blanidas 300 |
|---|---|---|---|---|---|
| Decontamination efficiency, % | royal frost | 56±6 | – | – | 78±5 |
| jacquemontii | 48±5 | – | – | 69±5 | |
| Morphogenesis of explants, % | royal frost | 31±4 | – | – | 54±3 |
| jacquemontii | 27±3 | – | – | 51±3 |