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Duration of plant regeneration in vitro from different types of primary explants, days
| Type of explant | Medium | ||
|---|---|---|---|
| MS | M1 | MK | |
| Meristem | 63 ± 7.2 | 57 ± 8.1 | 61 ± 7.0 |
| Bud | 39 ± 3.9 | 33 ± 4.8 | 30 ± 4.1 |
| Shoot stem | 34 ± 4.7 | 32 ± 4.2 | 30 ± 3.8 |
Peculiarities of adaptation of regenerants depending on the basis of the substrate
| Indicator | The basis of the substrate | |
|---|---|---|
| Peat | Perlite | |
| Survival, % | 73.3 ± 6.0 | 89.3 ± 6.3 |
| Height of regenerants on the 30th day, mm | 76.5 ± 6.0 | 67.1 ± 5.3 |
| Inhabitation by insects, pieces/container | 23.1 ± 6.4 | 4.0 ± 1.7 |
The influence of the nutrient medium on the rate of raspberry multiplication in vitro (BAP 1_0 mg/l, fifth passage)
| Indicator | Medium | ||
|---|---|---|---|
| MS | MK | M1 | |
| Multiplication coefficient | 4.7 ± 0.3 | 2.1 ± 0.3 | 5.6 ± 0.3 |
| Regeneration period, days | 32.0 ± 4.4 | 49.0 ± 4.9 | 24.0 ± 3.2 |
Effectiveness of controlling the quantity of insects in humid chambers
| Number of insects | Control | Standard | Insectivorous plants | ||
|---|---|---|---|---|---|
| Drosera | Dionaea | Nepenthes | |||
| Peat substrate | |||||
| Beginning | 9±3.2 | 10±3.0 | 9±2.9 | 11±4.2 | 8±2.8 |
| End | 27±4.9 | 7±1.8 | 3±0.9 | 19±4.0 | 23±5.7 |
| Perlite substrate | |||||
| Beginning | 3±0.9 | 3±0.6 | 4±0.6 | 3±0.4 | 4±1.1 |
| End | 11±2.8 | 5±1.2 | 1±0.2 | 9±1.9 | 8±2.9 |
The influence of the type of primary explants on the number of transfers and the yield of regenerants in vitro, medium M1
| Type of explant | Number of transfers, pieces | Yield of regenerants from primary explants, % |
|---|---|---|
| Meristem | 2.7±0.4 | 2.3±1.1 |
| Bud | 1.9±0.3 | 18.4±3.6 |
| Shoot stem | 1.5±0.3 | 54.3±4.1 |
The composition of nutrient media
| Component | Quantity, mg/l | ||
|---|---|---|---|
| MS | M1 | MK | |
| NH4NO3 | 1650.00 | 1250 | 417.00 |
| KNO3 | 1900.00 | 1100 | 367.00 |
| MgSO4 × 7H2O | 370.00 | 770 | 257.00 |
| KH2PO4 | 170.00 | 970 | 324.00 |
| Ca(NO3)2 × 4H2O | – | 440 | 293.00 |
| CaCl2 × 2H2O | 440.00 | – | – |
| FeSO4 × 7H2O | 27.80 | – | 18.54 |
| Na2MoO4 × 2H2O | 37.30 | – | 24.70 |
| Ferrilene 4.8 Orto–Orto | – | 183.4 | – |
| H3BO3 | 6.2 | ||
| MnSO4 × H2O | 22.3 | ||
| CoCl2 × 6H2O | 0.025 | ||
| CuSO4 × H2O | 0.025 | ||
| ZnSO4 × 7H2O | 8.6 | ||
| Na2MoO4 × 2H2O | 0.25 | ||
| KJ | 0.83 | ||
| Thiamine-HCl | 1.6 | ||
| Pyridoxine-HCl | 0.5 | ||
| Vitamin C | 2.0 | ||
| Nicotinic acid | 1.0 | ||
| Mesoinosit | 100 | ||
| Glycine | 0.5 | ||
| Adenine | 0.2 | ||
| Saccharose | 30,000 | ||
| Agar | 7,000 | ||
| pH 5.6 | |||
The influence of cultivation of donor plants during five passages on media with different concentrations of cytokinins on the rhizogenesis of regenerants
| Indicator | Cytokinin, mg/l | |||
|---|---|---|---|---|
| BAP 0.25 | BAP 1.0 | Kinetin 0.25 | Kinetin 1.0 | |
| Beginning of root formation, days | 19.0 ± 3.2 | 24.0 ± 3.7 | 13.0 ± 2.4 | 16.0 ± 2.9 |
| The length of the root system on the 30th day of cultivation, mm | 9.3 ± 3.0 | 4.2 ± 0.9 | 16.4 ± 3.1 | 14.1 ± 3.9 |
| The number of roots on the 30th day of cultivation | 9.2 ± 2.8 | 7.4 ± 2.2 | 7.3 ± 2.4 | 5.1 ± 1.8 |
The length of the raspberry root system ex vitro, mm
| Conditions | Cultivation day | |||
|---|---|---|---|---|
| 7 | 14 | 21 | 30 | |
| Wet chamber | – | 3±1.2 | 11±5.4 | 59±8.8 |
| Bioreactor | 27±3.2 | 68±7.0 | 103±9.1 | 124±11.0 |