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Comparison of bacterial communities in roots of selected trees with and without summer truffle (Tuber aestivum) ectomycorrhiza Cover

Comparison of bacterial communities in roots of selected trees with and without summer truffle (Tuber aestivum) ectomycorrhiza

Folia Forestalia Polonica
Volume 63 (2021): Issue 2 (June 2021)
By:  and    
Open Access
|Jun 2021
Figures & tables

Figures & Tables

Figure 1

Degree of root colonization expressed in % by bacteria, taking into account the research area (G, M, W), the variant (2, 3, 4, 5, 6), the season (autumn, spring) and year of evaluation (2017, 2018) for samples taken from Corylus avellana roots

Table 1

Average values of hydrothermal coefficient K and the average sum of precipitation for months IV–X at the nearest measuring stations in Kielce and Cracow in 2016–2018

Station nameKTotal precipitation by monthYears
IV–XI–XII
Kielce1.44378.6619.52016
Cracow1.95553.6745.3
Kielce2.27548.9731.82017
Cracow2.11545.9702.3
Kielce0.95340.1486.72018
Cracow1.25459.0568.7
Table 2

Percentage of bacteria of the dominant genera by season (S – spring; A – autumn) of the year (2017, 2018)

Genus20172018
SASA
Bacillus6.86.83.411.2
Erwinia9.60.03.43.4
Pseudomonas20.532.92.214.6
Rahnella1.41.430.30.0
Serratia4.14.122.50.0
Other6.85.56.72.2
Table 3

Percentage of bacteria of dominant genera, indicating variant (T – truffle; C– control) obtained by Sanger sequencing. The numbers in bold indicate the presence of a particular genus of bacteria in a particular sample

Genus20172018
TCTC
Bacillus6.86.86.77.9
Pseudomonas32.920.511.23.4
Erwinia8.21.45.6–
Rahnella–4.118.011.2
Serratia6.81.49.05.6
Brevibacterium1.4–––
Enterobacter1.41.41.1–
Viridibacillus1.41.4––
Microbacterium–1.4––
Pantoea–1.4––
Bradyrhizobium––2.2–
Acinetobacter––1.1–
Methylobacterium–––1.1
Viridibacillus–––1.1
Table 4

Alpha biodiversity index values for Chao, Faith’s, OTUs, Shannon’s, Heip’s and Simpson’s indices for selected samples representing sites W, G and M

SampleChaoFaith’sOTUShannon’sHeip’sSimpson’s
W1188.0013.77188.007.120.740.99
W2178.0013.53178.007.090.760.99
W4362.0014.56362.007.780.610.99
W5272.0013.36272.007.430.630.99
G1246.0013.44246.007.490.730.99
G2120.0012.74120.006.510.760.99
G3233.0015.23233.007.250.650.99
G4184.0012.87184.006.980.680.99
G5239.0013.73239.007.460.740.99
G6214.0013.48214.007.230.700.99
M1162.0012.44162.006.350.500.98
M2245.0015.21245.007.500.740.99
M3252.0014.80252.007.530.730.99
M4203.0013.38203.007.140.690.99
M5200.0013.54200.007.070.670.99
M6121.0011.12121.006.480.730.98
Figure 2

PcoA distance plots: A – Weighted UniFrac and B– unweighted UniFrac

Figure 3

PcoA distance plot – Bray-Curtis dissimilarity

Figure 4

PcoA distance plot – Jaccard index

Figure 5

Boxplot charts with Bray-Curtis (A), Unweighted (B), Jaccard (C), and Weighted (D) indicators for WC sample

OTU ID (genus)VARIANT
TC
123
Acidicapsa0834
Acidipila01813
Acidisphaera01306
Acidobacterium0759
Acidocella035
Acidothermus0184
Acidovorax060
Actinoallomurus0117
Actinocrinis0473
Actinokineospora079
Actinospica0717
Actinosynnema0206
Afipia2620
Agromyces900
Albimonas290
Aliidongia0955
Allokutzneria054
Alteromonas370
Amaricoccus550
Angustibacter0296
Arcobacter380
Asaia480
Azoarcus540
Azospirillum039
Bacillus310
Baekduia1450
Bauldia250
Bdellovibrio032
Blastococcus760
Blastomonas087
Bradyrhizobium0153
Brumimicrobium083
Bryobacter0297
Bryocella0112
Caenimonas760
Caldicellulosiruptor0102
Calditerricola014
Catelliglobosispora069
Catenulispora0308
Chelativorans1290
Chitinophaga3020
Chondromyces220
Collimonas041
Conexibacter0333
Denitratisoma710
Desulfonatronum1710
Desulfuromonas2800
Devosia0246
Dinghuibacter019
Dokdonella039
Dyadobacter028
Elioraea037
Erythrobacter910
Ferrimicrobium3170
Ferruginibacter2130
Fibrobacter450
Flavitalea0127
Frigoribacterium035
Goodfellowiella0151
Granulicella02639
Haliangium700
Hartmannibacter350
Helicobacter3370
Herbaspirillum022
Ideonella470
Inquilinus024
Jatrophihabitans0175
Jiangella2530
Kaistibacter0166
Kineosporia0126
Kofleria370
Krasilnikovia2460
Kutzneria092
Labedaea0173
Lentzea3060
Leptothrix0265
Lewinella1590
Lysobacter0282
Marmoricola037
Mesorhizobium2560
Methylobrevis660
Methyloceanibacter5560
Methylosinus0103
Methylotenera24690
Microcystis2110
Mycobacterium0107
Nakamurella630
Natranaerovirga740
Niabella2110
Niameybacter0108
Niastella4750
Nitratifractor0173
Nitrosospira8130
Nonomuraea065
Occallatibacter0249
Paludibaculum02367
Parasegetibacter1940
Pedobacter0126
Pelomonas830
Phaselicystis840
Phycicoccus031
Phytomonospora2140
Piscinibacter860
Plantactinospora3530
Polaromonas570
Promicromonospora1380
Providencia550
Pseudomonas028
Pseudonocardia0379
Pseudoxanthobacter056
Puia0508
Reyranella086
Rhizocola200
Rhizomicrobium0651
Rhizorhabdus25050
Rhodanobacter075
Rhodoferax3290
Rhodomicrobium3750
Rhodopila026
Rhodoplanes1480
Rhodopseudomonas0440
Roseiarcus03935
Rubrivivax049
Saccharomonospora1280
Salinispora900
Skermanella0165
Solirubrobacter0654
Sorangium300
Sphingobacterium3910
Sphingomonas8380
Stenotrophomonas018
Steroidobacter0143
Streptacidiphilus0494
Streptomyces6830
Sulfurisoma2000
Tabrizicola900
Telmatobacter05097
Terracidiphilus0448
Terriglobus083
Thermocatellispora085
Uliginosibacterium510
Umezawaea2070
Virgisporangium490
DOI: https://doi.org/10.2478/ffp-2021-0011 | Journal eISSN: 2199-5907 | Journal ISSN: 0071-6677
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Language: English
Page range: 97 - 111
Submitted on: Nov 30, 2020
Accepted on: Mar 30, 2021
Published on: Jun 8, 2021
Published by: Forest Research Institute
In partnership with: Paradigm Publishing Services
Keywords:
mycorrhiza,
NGS,
soil microorganisms,
summer truffle ascomata,
roots
Related subjects:
Life sciences,
Plant science

© 2021 Marta Siebyła, Iwona Szyp-Borowska, published by Forest Research Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 63 (2021): Issue 2 (June 2021)
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