Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Percentage of bacteria of the dominant genera by season (S – spring; A – autumn) of the year (2017, 2018)
| Genus | 2017 | 2018 | ||
|---|---|---|---|---|
| S | A | S | A | |
| Bacillus | 6.8 | 6.8 | 3.4 | 11.2 |
| Erwinia | 9.6 | 0.0 | 3.4 | 3.4 |
| Pseudomonas | 20.5 | 32.9 | 2.2 | 14.6 |
| Rahnella | 1.4 | 1.4 | 30.3 | 0.0 |
| Serratia | 4.1 | 4.1 | 22.5 | 0.0 |
| Other | 6.8 | 5.5 | 6.7 | 2.2 |
Average values of hydrothermal coefficient K and the average sum of precipitation for months IV–X at the nearest measuring stations in Kielce and Cracow in 2016–2018
| Station name | K | Total precipitation by month | Years | |
|---|---|---|---|---|
| IV–X | I–XII | |||
| Kielce | 1.44 | 378.6 | 619.5 | 2016 |
| Cracow | 1.95 | 553.6 | 745.3 | |
| Kielce | 2.27 | 548.9 | 731.8 | 2017 |
| Cracow | 2.11 | 545.9 | 702.3 | |
| Kielce | 0.95 | 340.1 | 486.7 | 2018 |
| Cracow | 1.25 | 459.0 | 568.7 | |
Percentage of bacteria of dominant genera, indicating variant (T – truffle; C– control) obtained by Sanger sequencing_ The numbers in bold indicate the presence of a particular genus of bacteria in a particular sample
| Genus | 2017 | 2018 | ||
|---|---|---|---|---|
| T | C | T | C | |
| Bacillus | 6.8 | 6.8 | 6.7 | 7.9 |
| Pseudomonas | 32.9 | 20.5 | 11.2 | 3.4 |
| Erwinia | 8.2 | 1.4 | 5.6 | – |
| Rahnella | – | 4.1 | 18.0 | 11.2 |
| Serratia | 6.8 | 1.4 | 9.0 | 5.6 |
| Brevibacterium | 1.4 | – | – | – |
| Enterobacter | 1.4 | 1.4 | 1.1 | – |
| Viridibacillus | 1.4 | 1.4 | – | – |
| Microbacterium | – | 1.4 | – | – |
| Pantoea | – | 1.4 | – | – |
| Bradyrhizobium | – | – | 2.2 | – |
| Acinetobacter | – | – | 1.1 | – |
| Methylobacterium | – | – | – | 1.1 |
| Viridibacillus | – | – | – | 1.1 |
Alpha biodiversity index values for Chao, Faith’s, OTUs, Shannon’s, Heip’s and Simpson’s indices for selected samples representing sites W, G and M
| Sample | Chao | Faith’s | OTU | Shannon’s | Heip’s | Simpson’s |
|---|---|---|---|---|---|---|
| W1 | 188.00 | 13.77 | 188.00 | 7.12 | 0.74 | 0.99 |
| W2 | 178.00 | 13.53 | 178.00 | 7.09 | 0.76 | 0.99 |
| W4 | 362.00 | 14.56 | 362.00 | 7.78 | 0.61 | 0.99 |
| W5 | 272.00 | 13.36 | 272.00 | 7.43 | 0.63 | 0.99 |
| G1 | 246.00 | 13.44 | 246.00 | 7.49 | 0.73 | 0.99 |
| G2 | 120.00 | 12.74 | 120.00 | 6.51 | 0.76 | 0.99 |
| G3 | 233.00 | 15.23 | 233.00 | 7.25 | 0.65 | 0.99 |
| G4 | 184.00 | 12.87 | 184.00 | 6.98 | 0.68 | 0.99 |
| G5 | 239.00 | 13.73 | 239.00 | 7.46 | 0.74 | 0.99 |
| G6 | 214.00 | 13.48 | 214.00 | 7.23 | 0.70 | 0.99 |
| M1 | 162.00 | 12.44 | 162.00 | 6.35 | 0.50 | 0.98 |
| M2 | 245.00 | 15.21 | 245.00 | 7.50 | 0.74 | 0.99 |
| M3 | 252.00 | 14.80 | 252.00 | 7.53 | 0.73 | 0.99 |
| M4 | 203.00 | 13.38 | 203.00 | 7.14 | 0.69 | 0.99 |
| M5 | 200.00 | 13.54 | 200.00 | 7.07 | 0.67 | 0.99 |
| M6 | 121.00 | 11.12 | 121.00 | 6.48 | 0.73 | 0.98 |
j_ffp-2021-0011_tab_005
| OTU ID (genus) | VARIANT | |
|---|---|---|
| T | C | |
| 1 | 2 | 3 |
| Acidicapsa | 0 | 834 |
| Acidipila | 0 | 1813 |
| Acidisphaera | 0 | 1306 |
| Acidobacterium | 0 | 759 |
| Acidocella | 0 | 35 |
| Acidothermus | 0 | 184 |
| Acidovorax | 0 | 60 |
| Actinoallomurus | 0 | 117 |
| Actinocrinis | 0 | 473 |
| Actinokineospora | 0 | 79 |
| Actinospica | 0 | 717 |
| Actinosynnema | 0 | 206 |
| Afipia | 262 | 0 |
| Agromyces | 90 | 0 |
| Albimonas | 29 | 0 |
| Aliidongia | 0 | 955 |
| Allokutzneria | 0 | 54 |
| Alteromonas | 37 | 0 |
| Amaricoccus | 55 | 0 |
| Angustibacter | 0 | 296 |
| Arcobacter | 38 | 0 |
| Asaia | 48 | 0 |
| Azoarcus | 54 | 0 |
| Azospirillum | 0 | 39 |
| Bacillus | 31 | 0 |
| Baekduia | 145 | 0 |
| Bauldia | 25 | 0 |
| Bdellovibrio | 0 | 32 |
| Blastococcus | 76 | 0 |
| Blastomonas | 0 | 87 |
| Bradyrhizobium | 0 | 153 |
| Brumimicrobium | 0 | 83 |
| Bryobacter | 0 | 297 |
| Bryocella | 0 | 112 |
| Caenimonas | 76 | 0 |
| Caldicellulosiruptor | 0 | 102 |
| Calditerricola | 0 | 14 |
| Catelliglobosispora | 0 | 69 |
| Catenulispora | 0 | 308 |
| Chelativorans | 129 | 0 |
| Chitinophaga | 302 | 0 |
| Chondromyces | 22 | 0 |
| Collimonas | 0 | 41 |
| Conexibacter | 0 | 333 |
| Denitratisoma | 71 | 0 |
| Desulfonatronum | 171 | 0 |
| Desulfuromonas | 280 | 0 |
| Devosia | 0 | 246 |
| Dinghuibacter | 0 | 19 |
| Dokdonella | 0 | 39 |
| Dyadobacter | 0 | 28 |
| Elioraea | 0 | 37 |
| Erythrobacter | 91 | 0 |
| Ferrimicrobium | 317 | 0 |
| Ferruginibacter | 213 | 0 |
| Fibrobacter | 45 | 0 |
| Flavitalea | 0 | 127 |
| Frigoribacterium | 0 | 35 |
| Goodfellowiella | 0 | 151 |
| Granulicella | 0 | 2639 |
| Haliangium | 70 | 0 |
| Hartmannibacter | 35 | 0 |
| Helicobacter | 337 | 0 |
| Herbaspirillum | 0 | 22 |
| Ideonella | 47 | 0 |
| Inquilinus | 0 | 24 |
| Jatrophihabitans | 0 | 175 |
| Jiangella | 253 | 0 |
| Kaistibacter | 0 | 166 |
| Kineosporia | 0 | 126 |
| Kofleria | 37 | 0 |
| Krasilnikovia | 246 | 0 |
| Kutzneria | 0 | 92 |
| Labedaea | 0 | 173 |
| Lentzea | 306 | 0 |
| Leptothrix | 0 | 265 |
| Lewinella | 159 | 0 |
| Lysobacter | 0 | 282 |
| Marmoricola | 0 | 37 |
| Mesorhizobium | 256 | 0 |
| Methylobrevis | 66 | 0 |
| Methyloceanibacter | 556 | 0 |
| Methylosinus | 0 | 103 |
| Methylotenera | 2469 | 0 |
| Microcystis | 211 | 0 |
| Mycobacterium | 0 | 107 |
| Nakamurella | 63 | 0 |
| Natranaerovirga | 74 | 0 |
| Niabella | 211 | 0 |
| Niameybacter | 0 | 108 |
| Niastella | 475 | 0 |
| Nitratifractor | 0 | 173 |
| Nitrosospira | 813 | 0 |
| Nonomuraea | 0 | 65 |
| Occallatibacter | 0 | 249 |
| Paludibaculum | 0 | 2367 |
| Parasegetibacter | 194 | 0 |
| Pedobacter | 0 | 126 |
| Pelomonas | 83 | 0 |
| Phaselicystis | 84 | 0 |
| Phycicoccus | 0 | 31 |
| Phytomonospora | 214 | 0 |
| Piscinibacter | 86 | 0 |
| Plantactinospora | 353 | 0 |
| Polaromonas | 57 | 0 |
| Promicromonospora | 138 | 0 |
| Providencia | 55 | 0 |
| Pseudomonas | 0 | 28 |
| Pseudonocardia | 0 | 379 |
| Pseudoxanthobacter | 0 | 56 |
| Puia | 0 | 508 |
| Reyranella | 0 | 86 |
| Rhizocola | 20 | 0 |
| Rhizomicrobium | 0 | 651 |
| Rhizorhabdus | 2505 | 0 |
| Rhodanobacter | 0 | 75 |
| Rhodoferax | 329 | 0 |
| Rhodomicrobium | 375 | 0 |
| Rhodopila | 0 | 26 |
| Rhodoplanes | 148 | 0 |
| Rhodopseudomonas | 0 | 440 |
| Roseiarcus | 0 | 3935 |
| Rubrivivax | 0 | 49 |
| Saccharomonospora | 128 | 0 |
| Salinispora | 90 | 0 |
| Skermanella | 0 | 165 |
| Solirubrobacter | 0 | 654 |
| Sorangium | 30 | 0 |
| Sphingobacterium | 391 | 0 |
| Sphingomonas | 838 | 0 |
| Stenotrophomonas | 0 | 18 |
| Steroidobacter | 0 | 143 |
| Streptacidiphilus | 0 | 494 |
| Streptomyces | 683 | 0 |
| Sulfurisoma | 200 | 0 |
| Tabrizicola | 90 | 0 |
| Telmatobacter | 0 | 5097 |
| Terracidiphilus | 0 | 448 |
| Terriglobus | 0 | 83 |
| Thermocatellispora | 0 | 85 |
| Uliginosibacterium | 51 | 0 |
| Umezawaea | 207 | 0 |
| Virgisporangium | 49 | 0 |