Table 1:
Thermal conditions for cDNA synthesis.
| Temperature | Time | Number of cycles |
|---|---|---|
| 95°C | 4 min | 1 |
| 94°C | 30 s | 35 |
| 57°C | 30 s | |
| 72°C | 30 s | |
| 72°C | 5 min | 1 |
Figure 1:
Cytotoxic potential of A) L actobacillus acidophilus, B) L actobacillus plantarum, and C) a combination of them on the MCF-7 cell line at various time points. *p < 0.05, **p < 0.01
Figure 2:
Comparison of the cytotoxic potential of A) Lactobacillus acidophilus and B) Lactobacillus plantarum at various time points. **Differences at p-value <0.05 were considered significant.
Figure 3:
IC50 values for the supernatants of A) Lactobacillus acidophilus, B) Lactobacillus plantarum, and C) a combination of the two probiotics.
Figure 4:
Cytotoxic potential of A) Lactobacillus acidophilus, B) Lactobacillus plantarum, and C) a combination of them on the MCF-10 A cell line after 24 h. Cytotoxicity evaluation of pellets of each probiotic and their combination showed the antiproliferative effects on both MCF-7 and MCF-10 A cells after 24, 48, and 72 h (data not shown). Therefore, no further investigations were conducted on the probiotic pellets.
Table 2:
Results of the cell viability test after treatment of MCF-7 cells with probiotics for 24 h
| Treatments | Cell viability percentage | Cell death percentage |
|---|---|---|
| Lactobacillus acidophilus supernatant 20% | 0 | 100 |
| Lactobacillus plantarum supernatant 10% | 0 | 100 |
| A mixture of both probiotic supernatants (20%) | 15.7 | 84.3 |
| MRS 20% | 89 | 11 |
Figure 5:
Effects of Lactobacillus acidophilus, Lactobacillus plantarum, and their combination (MIX) on apoptosis of MCF-7 and MCF-10 A cell lines after 24 h. **Differences at p-value <0.05 were considered significant.
Figure 6:
Effects of MRS culture medium, Lactobacillus acidophilus, Lactobacillus plantarum, and their combination on BCL2 expression in MCF-7 and MCF-10 A cell lines after 24 h. *Differences at p-value <0.05 were considered significant. MRS: de Man, Rogosa, and Sharpe medium.