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Effects of Lactobacillus acidophilus and Lactobacillus plantarum on Survival and Proliferation of Breast Cancer Cell Line MCF-7

Open Access
|Sep 2025

Figures & Tables

Figure 1:

Cytotoxic potential of A) L actobacillus acidophilus, B) L actobacillus plantarum, and C) a combination of them on the MCF-7 cell line at various time points. *p < 0.05, **p < 0.01
Cytotoxic potential of A) L actobacillus acidophilus, B) L actobacillus plantarum, and C) a combination of them on the MCF-7 cell line at various time points. *p < 0.05, **p < 0.01

Figure 2:

Comparison of the cytotoxic potential of A) Lactobacillus acidophilus and B) Lactobacillus plantarum at various time points. **Differences at p-value <0.05 were considered significant.
Comparison of the cytotoxic potential of A) Lactobacillus acidophilus and B) Lactobacillus plantarum at various time points. **Differences at p-value <0.05 were considered significant.

Figure 3:

IC50 values for the supernatants of A) Lactobacillus acidophilus, B) Lactobacillus plantarum, and C) a combination of the two probiotics.
IC50 values for the supernatants of A) Lactobacillus acidophilus, B) Lactobacillus plantarum, and C) a combination of the two probiotics.

Figure 4:

Cytotoxic potential of A) Lactobacillus acidophilus, B) Lactobacillus plantarum, and C) a combination of them on the MCF-10 A cell line after 24 h. Cytotoxicity evaluation of pellets of each probiotic and their combination showed the antiproliferative effects on both MCF-7 and MCF-10 A cells after 24, 48, and 72 h (data not shown). Therefore, no further investigations were conducted on the probiotic pellets.
Cytotoxic potential of A) Lactobacillus acidophilus, B) Lactobacillus plantarum, and C) a combination of them on the MCF-10 A cell line after 24 h. Cytotoxicity evaluation of pellets of each probiotic and their combination showed the antiproliferative effects on both MCF-7 and MCF-10 A cells after 24, 48, and 72 h (data not shown). Therefore, no further investigations were conducted on the probiotic pellets.

Figure 5:

Effects of Lactobacillus acidophilus, Lactobacillus plantarum, and their combination (MIX) on apoptosis of MCF-7 and MCF-10 A cell lines after 24 h. **Differences at p-value <0.05 were considered significant.
Effects of Lactobacillus acidophilus, Lactobacillus plantarum, and their combination (MIX) on apoptosis of MCF-7 and MCF-10 A cell lines after 24 h. **Differences at p-value <0.05 were considered significant.

Figure 6:

Effects of MRS culture medium, Lactobacillus acidophilus, Lactobacillus plantarum, and their combination on BCL2 expression in MCF-7 and MCF-10 A cell lines after 24 h. *Differences at p-value <0.05 were considered significant. MRS: de Man, Rogosa, and Sharpe medium.
Effects of MRS culture medium, Lactobacillus acidophilus, Lactobacillus plantarum, and their combination on BCL2 expression in MCF-7 and MCF-10 A cell lines after 24 h. *Differences at p-value <0.05 were considered significant. MRS: de Man, Rogosa, and Sharpe medium.

Results of the cell viability test after treatment of MCF-7 cells with probiotics for 24 h

TreatmentsCell viability percentageCell death percentage
Lactobacillus acidophilus supernatant 20%0100
Lactobacillus plantarum supernatant 10%0100
A mixture of both probiotic supernatants (20%)15.784.3
MRS 20%8911

Thermal conditions for cDNA synthesis_

TemperatureTimeNumber of cycles
95°C4 min1
94°C30 s35
57°C30 s
72°C30 s
72°C5 min1
DOI: https://doi.org/10.2478/fco-2024-0014 | Journal eISSN: 1792-362X | Journal ISSN: 1792-345X
Language: English
Submitted on: Dec 3, 2024
Accepted on: Jul 14, 2025
Published on: Sep 26, 2025
Published by: Helenic Society of Medical Oncology
In partnership with: Paradigm Publishing Services
Publication frequency: 2 times per year

© 2025 Sheida Jannati, Mona Roozbehani, Majid Khoshmirsafa, Shaghayegh Nasr, Sara Minaeian, Ahmad Tavakoli, Fatemeh Faraji, published by Helenic Society of Medical Oncology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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