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Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic Cover

Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

Open Access
|Jul 2021

Figures & Tables

Figure 1A

Diagnovital SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control).
Diagnovital SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control).

Figure 1B

Bio-Speedy SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control)
Bio-Speedy SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control)

Figure 2

Comparison of detection rate and Ct values of Diagnovital® kit detecting N1 and N2 genes and Bio-Speedy® kit detecting RdRp gene. Graph depicts Ct values obtained for all samples (n=43) in all RT-PCR assays. The lines show the mean Ct value for each assay, triangles show the Ct values of the samples with the highest (sample 28) and lowest (sample 22) value. The detection rate for each kit is indicated below the data points, e.g. 10/43 means 10 out of 43 samples tested positive according to the instructions for data interpretation provided by the manufacturer
Comparison of detection rate and Ct values of Diagnovital® kit detecting N1 and N2 genes and Bio-Speedy® kit detecting RdRp gene. Graph depicts Ct values obtained for all samples (n=43) in all RT-PCR assays. The lines show the mean Ct value for each assay, triangles show the Ct values of the samples with the highest (sample 28) and lowest (sample 22) value. The detection rate for each kit is indicated below the data points, e.g. 10/43 means 10 out of 43 samples tested positive according to the instructions for data interpretation provided by the manufacturer

The comparison of two different targeted COVID-19 RT-qPCR kits in positive specimens_

Sample NoRT-qPCR Cycle Thhreshold Value (Ct)
Diagnovital® N1+N2Bio-Speedy® RdRp
132.98Negative
232Negative
330.22Negative
438.96Negative
520.3335.33
630.60Negative
733.3633.71
836.36Negative
934.01Negative
1027.9333.89
122.0227.17
1237.93Negative
1330.71Negative
1429.8834.19
1528.4130.20
1638.41Negative
1729.34Negative
1831.44Negative
1936.24Negative
2035.81Negative
2136.80Negative
221.5528.19
2329.95Negative
2436.68Negative
2537.64Negative
2633.39Negative
2728.73Negative
2840.00Negative
2937.07Negative
3032.42Negative
3137.03Negative
3232.21Negative
333.10Negative
3425.5434.73
3522.2531.15
3628.29Negative
3733.05Negative
3839.94Negative
3932.75Negative
4026.2135.21
4134.45Negative
4228.18Negative
4337.27Negative
PC 128.6027.57
PC 228.2826.20
Total4310
Language: English
Page range: 26 - 31
Published on: Jul 24, 2021
Published by: European Biotechnology Thematic Network Association
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2021 Gulten Tuncel, Mahmut Cerkez Ergoren, Buket Baddal, Pinar Tulay, Ayse Arikan, Emrah Guler, Cenk Serhan Ozverel, H. Kaya Suer, Murat Sayan, Tamer Sanlidag, , published by European Biotechnology Thematic Network Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.