Table 1
The comparison of two different targeted COVID-19 RT-qPCR kits in positive specimens.
| Sample No | RT-qPCR Cycle Thhreshold Value (Ct) | |
|---|---|---|
| Diagnovital® N1+N2 | Bio-Speedy® RdRp | |
| 1 | 32.98 | Negative |
| 2 | 32 | Negative |
| 3 | 30.22 | Negative |
| 4 | 38.96 | Negative |
| 5 | 20.33 | 35.33 |
| 6 | 30.60 | Negative |
| 7 | 33.36 | 33.71 |
| 8 | 36.36 | Negative |
| 9 | 34.01 | Negative |
| 10 | 27.93 | 33.89 |
| 1 | 22.02 | 27.17 |
| 12 | 37.93 | Negative |
| 13 | 30.71 | Negative |
| 14 | 29.88 | 34.19 |
| 15 | 28.41 | 30.20 |
| 16 | 38.41 | Negative |
| 17 | 29.34 | Negative |
| 18 | 31.44 | Negative |
| 19 | 36.24 | Negative |
| 20 | 35.81 | Negative |
| 21 | 36.80 | Negative |
| 2 | 21.55 | 28.19 |
| 23 | 29.95 | Negative |
| 24 | 36.68 | Negative |
| 25 | 37.64 | Negative |
| 26 | 33.39 | Negative |
| 27 | 28.73 | Negative |
| 28 | 40.00 | Negative |
| 29 | 37.07 | Negative |
| 30 | 32.42 | Negative |
| 31 | 37.03 | Negative |
| 32 | 32.21 | Negative |
| 3 | 33.10 | Negative |
| 34 | 25.54 | 34.73 |
| 35 | 22.25 | 31.15 |
| 36 | 28.29 | Negative |
| 37 | 33.05 | Negative |
| 38 | 39.94 | Negative |
| 39 | 32.75 | Negative |
| 40 | 26.21 | 35.21 |
| 41 | 34.45 | Negative |
| 42 | 28.18 | Negative |
| 43 | 37.27 | Negative |
| PC 1 | 28.60 | 27.57 |
| PC 2 | 28.28 | 26.20 |
| Total | 43 | 10 |
Figure 1A
Diagnovital SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control).
Figure 1B
Bio-Speedy SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control)
Figure 2
Comparison of detection rate and Ct values of Diagnovital® kit detecting N1 and N2 genes and Bio-Speedy® kit detecting RdRp gene. Graph depicts Ct values obtained for all samples (n=43) in all RT-PCR assays. The lines show the mean Ct value for each assay, triangles show the Ct values of the samples with the highest (sample 28) and lowest (sample 22) value. The detection rate for each kit is indicated below the data points, e.g. 10/43 means 10 out of 43 samples tested positive according to the instructions for data interpretation provided by the manufacturer