Table 1.
Clinicopathological characteristics of breast cancer
| Variables | N | % | |
|---|---|---|---|
| Side | left | 66 | 44.9 |
| right | 81 | 55.1 | |
| Histological type | lobular | 18 | 12.4 |
| ductal | 123 | 84.8 | |
| other | 4 | 2.8 | |
| Histological grade | HG1 | 17 | 11.9 |
| HG2 | 73 | 51 | |
| HG3 | 53 | 37.1 | |
| Nuclear grade | NG1 | 17 | 15.2 |
| NG2 | 64 | 57.1 | |
| NG3 | 31 | 27.7 | |
| Mitotic index | grade 1 | 19 | 42.2 |
| grade 2 | 20 | 44.4 | |
| grade 3 | 6 | 13.3 | |
| Tumor necrosis | absent | 26 | 21.7 |
| present | 94 | 78.3 | |
| Desmoplasia | low | 17 | 16.3 |
| medium | 55 | 52.9 | |
| high | 32 | 30.8 | |
| Periductal elastosis | low | 19 | 20.0 |
| medium | 20 | 44.4 | |
| high | 16 | 35.6 | |
| Perineural invasion | absent | 101 | 68.7 |
| present | 46 | 31.3 | |
| Lymphatic invasion | absent | 72 | 48.9 |
| present | 75 | 51.1 | |
| Vascular invasion | absent | 113 | 76.9 |
| present | 34 | 23.1 | |
| HER2 | negative | 115 | 79.3 |
| positive | 30 | 20.7 | |
| Ki67 | low | 30 | 20..9 |
| medium | 42 | 29.4 | |
| high | 71 | 49.7 | |
| Molecular subtypes | Lum A | 30 | 20.4 |
| Lum B | 76 | 51.7 | |
| HER2 + | 19 | 12.9 | |
| TNBC | 22 | 15 | |
| T status | T1 | 48 | 35.8 |
| T2 | 64 | 47.8 | |
| T3 | 9 | 6.7 | |
| T4 | 13 | 9.7 | |
| N status | N0 | 50 | 37.3 |
| N1 | 48 | 35.8 | |
| N2 | 19 | 14.2 | |
| N3 | 17 | 12.7 | |
Figure 1.
Expression of GLB1 in association with cytological alterations within the epithelium.
Figure 2.
Correlation of GLB1 expression between groups in relation to cytological changes in epithelium.
Figure 3.
GLB1 expression in different molecular subtypes of IBC.
Figure 4.
Impact of Ki67 and HER2 Expression on GLB1 Levels in Tumor Cells
Figure 5.
GLB1 expression dependent on ER and PR expression.
Figure 6.
Receiver Operating Characteristic (ROC) curve analysis of GLB1 expression levels in Non-Invasive Lesions and Inflammatory Breast Cancer.
Figure 7.
A. Frequency of GLB1+ and GLB1− IBC in relation to GLB1 expression threshold. Microscopic image of GLB1 expression relative to threshold: B. GLB1+ and C. GLB1− (immunohistochemical analysis, original magnification 200x).
Table 2.
Association between GLB1 expression in IBC and examined clinicopathological characteristics
| Variables | GLB1 cut off 27,5% | Chi-Square | p | ||
|---|---|---|---|---|---|
| Mononuclear infiltrate | absent | 2 (6,5%) | 2 (3,2%) | 3,892 | 0,273 |
| low | 10 (32,3%) | 33 (53,2%) | |||
| medium | 14 (45,2%) | 21 (33,9%) | |||
| high | 5 (16,1%) | 6 (9,7%) | |||
| Stromal fibroblasts | negative | 4 (22,2%) | 13 (17,3%) | 0,020 | 0,887 |
| positive | 14 (77,8%) | 62 (82,7%) | |||
| Histological type | lobular | 4 (10,8%) | 14 (13,0%) | 0,120 | 0,942 |
| ductal | 32 (86,5%) | 91 (84,3%) | |||
| other | 1 (2,7%) | 3 (2,8%) | |||
| Histological grade | HG1 | 2 (5,3%) | 15 (14,3%) | 10,016 | 0,007 |
| HG2 | 14 (36,8%) | 59 (56,2%) | |||
| HG3 | 22 (57,9%) | 31 (29,5%) | |||
| Nuclear grade | NG1 | 2 (6,7%) | 15 (18,3%) | 8,080 | 0,018 |
| NG2 | 14 (46,7%) | 50 (61,0%) | |||
| NG3 | 14 (46,7%) | 17 (20,7) | |||
| Tumor necrosis | absent | 5 (16,7%) | 21 (23,3%) | 0,262 | 0,609 |
| present | 25 (83,3%) | 69 (76,7) | |||
| Perineural invasion | absent | 27 (71,1%) | 74 (67,9%) | 0,025 | 0,874 |
| present | 11 (28,9%) | 35 (32,1%) | |||
| Lymphatic invasion | absent | 14 (36,8%) | 58 (53,2%) | 2,402 | 0,121 |
| present | 24 (63,2%) | 51 (46,8%) | |||
| Vascular invasion | absent | 31 (81,6%) | 82 (75,2%) | 0,639 | 0,424 |
| present | 7 (18,4%) | 27 (24,8%) | |||
| Molecular subtypes | Lum A | 5 (13,2%) | 25 (22,9%) | 67,563 | 0,000 |
| Lum B | 12 (31,6%) | 64 (58,7%) | |||
| HER2 + | 0 (0,0%) | 19 (17,4%) | |||
| TNBC | 21 (55,3%) | 1 (0,9%) | |||
| HER2 | negative | 36 (94,7%) | 79 (73,%) | 6,249 | 0,012 |
| positive | 2 (5,3%) | 28 (26,2%) | |||
| Ki67 | low | 7 (20,0%) | 23 (21,3%) | 0,433 | 0,805 |
| medium | 9 (25,7%) | 33 (30,6%) | |||
| high | 19 (54,3%) | 52 (48,1%) | |||
| T status | T1 | 10 (28,6%) | 38 (38,4%) | 2,457 | 0,507 |
| T2 | 18 (51,4%) | 46 (46,5%) | |||
| T3 | 4 (11,4%) | 5 (5,1%) | |||
| T4 | 3 (8,6%) | 10 (10,1%) | |||
| N status | N0 | 9 (25,0%) | 41 (41,8%) | 4,862 | 0,182 |
| N1 | 13 (36,1%) | 35 (35,7%) | |||
| N2 | 8 (22,2%) | 11 (11,2%) | |||
| N3 | 6 (16,7%) | 11 (11,2%) | |||