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Effect of Hyperbaric Oxygenation on the Severity of Experimental Autoimmune Myocarditis in Gal-3 Deficient Mice Cover

Effect of Hyperbaric Oxygenation on the Severity of Experimental Autoimmune Myocarditis in Gal-3 Deficient Mice

Open Access
|Feb 2025

Figures & Tables

Figure 1.

Effects of HBO treatment on EAM severity score (A), degree of degeneration and necrosis of cardiomyocytes (B), and degree of fibrosis (C). CTRL: healthy control Gal-3KO mice; EAM: Gal-3KO mice with induced EAM; EAM+HBO: Gal-3KO mice with induced EAM and HBO treatment. Values are presented as means ± mean standard error (SE). Independent Samples t test; One-Way ANOVA *p< 0.05 indicates statistical significant differences between groups.
Effects of HBO treatment on EAM severity score (A), degree of degeneration and necrosis of cardiomyocytes (B), and degree of fibrosis (C). CTRL: healthy control Gal-3KO mice; EAM: Gal-3KO mice with induced EAM; EAM+HBO: Gal-3KO mice with induced EAM and HBO treatment. Values are presented as means ± mean standard error (SE). Independent Samples t test; One-Way ANOVA *p< 0.05 indicates statistical significant differences between groups.

Figure 2.

Representative images of hematoxylin/eosin (H&E) staining of paraffin embedded heart tissue sections. CTRL: healthy control Gal-3KO mice; EAM: Gal-3KO mice with induced EAM; EAM+HBO: Gal-3KO mice with induced EAM and HBO treatment. Original magnification 40×.
Representative images of hematoxylin/eosin (H&E) staining of paraffin embedded heart tissue sections. CTRL: healthy control Gal-3KO mice; EAM: Gal-3KO mice with induced EAM; EAM+HBO: Gal-3KO mice with induced EAM and HBO treatment. Original magnification 40×.

Effects of HBO treatment on echocardiographic parameters: interventricular septal wall thickness at end systole and end-diastole (IVSs and IVSd), left ventricular internal diameter at end-systole and end-diastole (LVIDs and LVIDs), left ventricular posterior wall thickness at endsystole and end-diastole (LVPWs and LVPWd), and fractional shortening (FS)_ CTRL: healthy control Gal-3KO mice; EAM: Gal-3KO mice with induced EAM; EAM+HBO: Gal-3KO mice with induced EAM and HBO treatment_ Values are presented as means ± mean standard error (SE)_ Kruskal–Wallis H; Mann–Whitney U tests *p< 0_05 indicates statistical significant differences between groups #compared to CTRL, *compared to EAM_

CTRLEAMEAM+HBO
IVSd (cm)0.064±0.0520.093±0.1010.100±0.032
LVIDd (cm)0.246±0.1770.210±0.1340.203±0.024*
LVPWd (cm)0.058±0.0620.086±0.1100.092±0.016
IVSs (cm)0.145±0.0340.141±0.0130.161±0.039
LVIDs (cm)0.090±0.0370.137±0.0170.097±0.047*
LVPWs (cm)0.103±0.0230.080±0.0240.107±0.020
FS (%)63.9±2.24143.6±5.51#52.5±4.84*
DOI: https://doi.org/10.2478/eabr-2024-0060 | Journal eISSN: 2956-2090 | Journal ISSN: 2956-0454
Language: English
Submitted on: Mar 15, 2023
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Accepted on: May 20, 2023
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Published on: Feb 6, 2025
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Katarina Milincic, Marina Miletic Kovacevic, Dragan Dulovic, Biljana Ljujic, published by University of Kragujevac, Faculty of Medical Sciences
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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