Determination of caffeic acid in root and rhizome of Black cohosh (Cimicifuga racemosa (L.) Nutt.)

Karolina Zapala; Grazyna Szymczak; Magdalena Wojciak-Kosior

doi:10.2478/cipms-2014-0025

Abstract

Cimicifuga racemosa, is a plant with a diverse and long history of medicinal use. Caffeic acid, bioactive compound, which often occurs with other polyphenols can influence the biological activity of this plant. The aim of our work was quantitative analysis of caffeic acid in roots and rhizomes of two varieties of C. racemosa. Analysis was performed by HPLC method. The extracts were separated on C18 reversed-phase column using mixture of methanol, water and formic acid (25:75:0.5 v/v/v) as a mobile phase. The flow rate of eluent was 1.0 ml·min^-1. The obtained validation parameters such as linearity, linear regression equation and precision expressed as a relative standard deviation were adequate for quantitative determination. Caffeic acid was found in all tested extracts. The highest total amount of caffeic acid was determined in C. racemosa var. racemosa (255.3 μg·g^-1) while its concentration in C. racemosa var. cordifolia was significantly lower (213.0 μg·g^-1).

References

1. Anwar J. et al.: Effects of caffeic acid on behavioral parameters and on the activity of acetylcholinesterase in different tissues from adult rats. Pharmacol. Biochem. Behav., 103, 386, 2012.10.1016/j.pbb.2012.09.006
Search in Google Scholar
2. Gulcin I.: Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology 217, 213, 2006.10.1016/j.tox.2005.09.011
Search in Google Scholar
3. Hui Z. et al.: Effects of black cohosh and estrogen on the hypothalamic nuclei of ovariectomized rats at different temperatures. J. Ethnopharmacol. 142, 769, 2012.10.1016/j.jep.2012.05.059
Search in Google Scholar
4. Jiang B. et al.: The value of plant collections in ethnopharmacology: a case study of an 85-year-old black cohosh (Actaea racemosa L.) sample. J. Ethnopharmacol. 96, 521, 2005.10.1016/j.jep.2004.09.049
Search in Google Scholar
5. Khan A.Q. et al.: Caffeic acid attenuates 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced NF- κB and COX-2 expression in mouse skin: abrogation of oxidativestress, inflammatory responses and proinflammatory cytokine production. Food Chem. Toxicol. 50, 175, 2012.10.1016/j.fct.2011.10.043
Search in Google Scholar
6. Leite F. R. F., Santos W. de J. R., Kubota L. T.: Selective determination of caffeic acid in wines with electrochemical sensor based on molecularly imprinted siloxanes. Sensors and Actuators B: Chemical 193, 238, 2014.
Search in Google Scholar
7. Low Dog T., Powell K.L., Weisman S.M.: Critical evaluation of the safety of Cimicifuga racemosa in menopause symptom relief. Menopause 10, 299, 2003.10.1097/01.GME.0000056039.51813.21
Search in Google Scholar
8. Margaret R.: Alternative treatments for the menopause. Clin. Obstet. Gynecol. 23, 151, 2009.10.1016/j.bpobgyn.2008.10.006
Search in Google Scholar
9. Naczk M., Shahidi F.: Exctraction and analysis of phenolics in food. J. Chromatogr. A 1054, 95, 2004.10.1016/S0021-9673(04)01409-8
Search in Google Scholar
10. Oh J. et al.: Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control 31, 403, 2013.10.1016/j.foodcont.2012.10.021
Search in Google Scholar
11. Tomas-Barberan F.A. et al.: HPLC - DAD - ESIMS analysis of phenolic compounds in nectarines, peaches, and plums. J. Agric. and Food Chem. 49, 4748, 2001.10.1021/jf010468111600017
Search in Google Scholar
12. Weng C., Yen G.: Chemopreventive effects of dietary phytochemicals against cancer invasion and metastasis: phenolic acids, monophenol, polyphenol, and their derivatives. Cancer Treat. Rev. 38, 76, 2012.10.1016/j.ctrv.2011.03.00121481535
Search in Google Scholar
13. Xie Y. et al.: Caffeic acid derivatives: A new type of influenza neuraminidase inhibitors. Bioorg. Med. Chem. 23, 3556, 2013.10.1016/j.bmcl.2013.04.03323664211
Search in Google Scholar

Determination of caffeic acid in root and rhizome of Black cohosh (Cimicifuga racemosa (L.) Nutt.)

Abstract

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