Antimicrobial susceptibility pattern and SCCmec types of methicillin-resistant coagulase-negative staphylococci from subclinical bovine mastitis in Hatay, Turkey

Aslantaş, Özkan; Yılmaz, Mehmet Ali; Yılmaz, Ebru Şebnem; Kurekci, Cemil

doi:10.2478/bvip-2014-0087

Abstract

Eighty-nine isolates of coagulase-negative staphylococci (CoNS) of eight species from subclinical bovine mastitis were screened for the phenotypic and genotypic methicilline-resistance. In addition, all methicillin-resistant (MR) isolates indicating the mecA gene were examined by PCR for the antimicrobial susceptibility patterns, and staphylococcal cassette chromosome mec (SCCmec) types were also determined by multiplex PCR. A total of 21 (23.6%) CoNS isolates were found to be resistant to oxacillin in broth microdilution assay. All isolates phenotypically resistant to oxacillin did not have the mecA gene, which was only found in 14.6% (13) of the isolates. Most MR-CoNS isolates were highly resistant to erythromycin (92.3%), fusidic acid (84.6%), penicillin (76.9%), and rifampycin (61.5%), and susceptible to mupirocin (100%), tetracycline (100%), vancomycin (100%), clindamycin (92.3%), and sulfamethoxazole-trimethoprim (69.2%). In conclusion, a high rate of antimicrobial resistance among MR-CoNS isolated from food producing animals emphasises the need for periodic surveillance of their resistance.

References

1. Choi S.M., Kim S.H., Kim H.J., Lee D.G., Choi J.H., Yoo J.H., Kang J.H., Shin W.S., Kang M.W.: Multiplex PCR for the detection of genes encoding aminoglycoside modifying enzymes and methicillin resistance among Staphylococcus species. J Korean Med Sci 2003, 18, 631-636.10.3346/jkms.2003.18.5.631
Search in Google Scholar
2. CLSI.: Performance Standards for Antimicrobial Susceptibility Testing, Eighteenth Informational Supplement, CLSI document M100- S18, Wayne, USA, 2008.
Search in Google Scholar
3. Feßler A.T., Billerbeck C.: Identification and characterization of methicillin-resistant coagulase-negative staphylococci from bovine mastitis. J Antimicrob Chemoth 2010, 65, 1576-1582.10.1093/jac/dkq172
Search in Google Scholar
4. Fuchs P.C., Jones R.N., Barry A.L.: Interpretive criteria for disk diffusion susceptibility testing of mupirocin, a topical antibiotic. J Clin Microbiol 1990, 28, 608-609.10.1128/jcm.28.3.608-609.1990
Search in Google Scholar
5. Hanssen A.M., Sollid J.U.E.: SCCmec in staphylococci: genes on the move. FEMS Immunol Med Microbiol 2006, 46, 8-20.10.1111/j.1574-695X.2005.00009.x
Search in Google Scholar
6. Hesselbarth J., Schwarz S.: Comparative ribotyping of Staphylococcus intermedius from dogs, pigeons, horses and mink. Vet Microbiol 1995, 45, 11-17.10.1016/0378-1135(94)00125-G
Search in Google Scholar
7. Huber H., Ziegler D., Pfluger V., Vogel G., Zweifel C., Stephan R.: Prevalence and characteristics of methicillinresistant coagulase-negative staphylococci from livestock, chicken carcasses, bulk tank milk, minced meat and contact persons. BMC Vet Res 2011, 7:6.10.1186/1746-6148-7-6304240221272304
Search in Google Scholar
8. Huebner J., Goldmann D.A.: Coagulase-negative staphylococci: role as pathogens. Ann Rev Med 1999, 50, 223-236.10.1146/annurev.med.50.1.22310073274
Search in Google Scholar
9. Ibrahem S., Salmenlinna S., Virolainen A., Kerttula A.M., Lyytikainen O., Jagerroos H., Broas M., Vuopio-Varkila J.: Carriage of methicillin-resistant staphylococci and their SCCmec types in a long-term-care facility. J Clin Microbiol 2009, 47, 32-37.10.1128/JCM.01085-08262085818971358
Search in Google Scholar
10. Inegöl E., Türkyılmaz S.: Determination of SCCmec types in methicillin resistant staphylococci isolated from cows and farm workers. Ankara Univ Vet Fak 2012, 59, 89-93.10.1501/Vetfak_0000002508
Search in Google Scholar
11. Juhász-Kaszanyitzky Jánosi É., S., Somogyi P., Dán Á., van der Graaf-van Bloois L., van Duijkeren E., Wagenaar J.A.: MRSA transmission between cows and humans. Emerg Infect Dis 2007, 13, 630-632.10.3201/eid1304.060833272596017553285
Search in Google Scholar
12. Koksal F., Yasar H., Samasti M.: Antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in Turkey. Microbiol Res 2009, 164, 404-410.10.1016/j.micres.2007.03.004
Search in Google Scholar
13. Kondo Y., Ito T., Ma X.X., Watanabe S., B Kreiswirth.N., Etienne J., Hiramatsu K.: Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Ch 2007, 51, 264-274.10.1128/AAC.00165-06
Search in Google Scholar
14. Luthje P., Schwarz S.: Antimicrobial resistance of coagulasenegative staphylococci from bovine subclinical mastitis with particular reference to macrolide lincosamide resistance phenotypes and genotypes. J Antimicrob Chemoth 2006, 57, 966-969.10.1093/jac/dkl061
Search in Google Scholar
15. Paradisi F., G. Corti, Messeri D.: Antistaphylococcal (MSSA, MRSA, MSSE, MRSE) antibiotics. Med Clin N Am 2001, 85, 1-17.10.1016/S0025-7125(05)70302-3
Search in Google Scholar
16. Pyorala S., Taponen S.: Coagulase-negative staphylococciemerging mastitis pathogens. Vet Microbiol 2009, 134, 3-8.10.1016/j.vetmic.2008.09.01518848410
Search in Google Scholar
17. Rajala-Schultz P.J., Torres A.H., DeGraves F.J., Gebreyes W.A., Patchanee P.: Antimicrobial resistance and genotypic characterization of coagulase-negative staphylococci over the dry period. Vet Microbiol 2009, 134, 55-64.10.1016/j.vetmic.2008.09.00818950963
Search in Google Scholar
18. Ruppe E., Barbier F., Mesli Y., Maiga A., Cojocaru R., Benkhalfat M., Benchouk S., Hassaine H., Maiga I., Diallo A., Koumare A.K., Ouattara K., Soumare S., Dufourcq J.B., Nareth C., Sarthou J.L., Andremont A., Ruimy R.: Diversity of Staphylococcal cassette chromosome mec structures in methicillin-resistant Staphylococcus epidermidis and Staphylococcus haemolyticus strains among outpatients from four countries. Antimicrob Agents CH 2009, 53, 442-449.10.1128/AAC.00724-08263065119001111
Search in Google Scholar
19. Sampimon O.C., Lam T.J.G.M., Mevius D.J., Schukken Y.H., Zadoks R.N.: Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine milk samples. Vet Microbiol 2011, 150, 173-179.10.1016/j.vetmic.2011.01.01721333468
Search in Google Scholar
20. Taponen S., Pyorla S.: Coagulase-negative staphylococci as cause of bovine mastitis-Not so different from Staphylococcus aureus. Vet Microbiol 2009, 134, 29-36.10.1016/j.vetmic.2008.09.01118977615
Search in Google Scholar
21. Toma E., Barriault D.: Antimicrobial activity of fusidic acid and disk diffusion susceptibility testing criteria for gram-positive cocci. J Clin Microbiol 1995, 33, 1712-1715.10.1128/jcm.33.7.1712-1715.19952282547665633
Search in Google Scholar
22. Ünal N., Çınar O.D.: Detection of stapylococcal enterotoxin, methicillin-resistant and Panton-Valentine leukocidin genes in coagulase-negative staphylococci isolated from cows and ewes with subclinical mastitis. Trop Anim Health Prod 2012, 44, 369-375.10.1007/s11250-011-0032-x22160510
Search in Google Scholar
23. Vanderhaeghen W., Cerpentier T., Adriaensen C., Vicca J., Hermans K., Butaye P.: Methicillin-resistant Staphylococcus aureus (MRSA) ST398 associated with clinical and subclinical mastitis in Belgian cows. Vet Microbiol 2010, 144, 166-171.10.1016/j.vetmic.2009.12.04420092969
Search in Google Scholar
24. Zhang Y., Agidi S., LeJeune J.T.: Diversity of staphylococcal cassette chromosome in coagulase-negative staphylococci from animal sources. J Appl Microbiol 2009, 107, 1375-1383.10.1111/j.1365-2672.2009.04322.x19486384
Search in Google Scholar

Antimicrobial susceptibility pattern and SCCmec types of methicillin-resistant coagulase-negative staphylococci from subclinical bovine mastitis in Hatay, Turkey

Abstract

Paradigm

My account